Portable, Low-Cost, Column Carbon Dioxide and Methane Measurements for Validating Satellite Observations in Remote Locations

We present a low-cost (~$10K per instrument), portable solution to ground-based validation of satellite observations for difficult to reach locations with precisions of 1 ppm XCO2 and 10 ppb XCH4 for hourly data products. While Total Carbon Column Observing Network (TCCON) is the gold-standard for ground validation, there are locations where a ground column validation data point would be useful but conditions are not conducive to a permanent station. Examples include wetlands, thawing permafrost, the tropics, the Amazon, sub-Saharan Africa, as well as locations without a power grid or with geopolitical conflict. In addition, the low-cost and portability mean a geographical region can be studied in depth with multiple instruments. This passive, sun-pointing instrument is a miniaturized, laser heterodyne radiometer (mini-LHR) that has been under development by our team since 2009. It can be operated either in tandem with AERONET (a global network of 500 instruments that measure aerosol optical depth), or as a stand-alone instrument with a low-cost (~$3K), light-weight sun tracker. One of the main benefits of the mini-LHR is that it can quickly reach remote locations and provide a validation measurement even if there is limited or no infrastructure at the site. The instrument weighs ~10 lbs, fits into a backpack, and is powered by two folding solar panels and a battery pack. In clear conditions, the instrument can be set-up in under an hour. Portability means that mini-LHRs can be easily moved for side-by-side comparisons with other mini-LHRs and with TCCON which simplifies assessing instrument bias as well as accuracy. Like TCCON, the mini-LHR points directly at the sun with a narrow field-of-view and is its insensitivity to cloud and aerosol scattering that is common to nadir-pointing passive satellite approaches. Here we present a collection of sample data sets to demonstrate performance from locations that vary in climate, altitude, solar zenith angle, hours of sun per day, etc., as well as data from side-by-side TCCON comparisons. Retrievals of CO2 and CH4 were completed using the NASA/Goddard's Planetary Spectrum Generator (PSG) that incorporates meteorological inputs from Modern-Era Retrospective analysis for Research and Applications, Version 2 (MERRA-2) data set

A GMSK VHF-uplink/UHF-downlink transceiver for the CubeSat missions: Thermo-functional performance

© 2018, CEAS. Functional and thermal performance characteristics of a very high frequency/ultra high frequency (VHF/UHF) transceiver based on Gaussian minimum shift keying (GMSK) modulation are presented. The transceiver has been designed for CubeSats telemetry and commanding needs or low rate data download. The design is validated at 27 dBm, 30 dBm and 33 dBm transmitting powers over −20 ∘C to +51 ∘C. Under these thermal conditions, the transmitter spurious dynamic response shows little if any change and the average sensitivity of receiver at the 12 dB signal noise and distortion (SINAD) is −116.7 dBm at 140 MHz and −116.78 dBm at 149.98 MHz. The transmitter and receiver frequencies are stable and the current consumption as well the output RF levels are steady. The design has been verified against a simulation model which allows system tradeoff analysis. The measurements demonstrate the transceiver made with commercial grade parts has dependable performance at the low earth altitudes and orbital heating conditions

Asymptotic Normalization Coefficients for 13C+p->14N

The $^{13}C(^{14}N,^{13}C)^{14}N$ proton exchange reaction has been measured at an incident energy of 162 MeV. Angular distributions were obtained for proton transfer to the ground and low lying excited states in $^{14}N$. Elastic scattering of $^{14}N$ on $^{13}C$ also was measured out to the rainbow angle region in order to find reliable optical model potentials. Asymptotic normalization coefficients for the system $^{13}C+p\to {}^{14}N$ have been found for the ground state and the excited states at 2.313, 3.948, 5.106 and 5.834 MeV in $^{14}N$. These asymptotic normalization coefficients will be used in a determination of the S-factor for $^{7}Be(p,\gamma)^{8}B$ at solar energies from a measurement of the proton transfer reaction $^{14}N(^{7}Be,^{8}B)^{13}C$.Comment: 5 pages, 6 figure

STIM1, an essential and conserved component of store-operated Ca2+ channel function

Store-operated Ca2+ (SOC) channels regulate many cellular processes, but the underlying molecular components are not well defined. Using an RNA interference (RNAi)-based screen to identify genes that alter thapsigargin (TG)-dependent Ca2+ entry, we discovered a required and conserved role of Stim in SOC influx. RNAi-mediated knockdown of Stim in Drosophila S2 cells significantly reduced TG-dependent Ca2+ entry. Patch-clamp recording revealed nearly complete suppression of the Drosophila Ca2+ release-activated Ca2+ (CRAC) current that has biophysical characteristics similar to CRAC current in human T cells. Similarly, knockdown of the human homologue STIM1 significantly reduced CRAC channel activity in Jurkat T cells. RNAi-mediated knockdown of STIM1 inhibited TG- or agonist-dependent Ca2+ entry in HEK293 or SH-SY5Y cells. Conversely, overexpression of STIM1 in HEK293 cells modestly enhanced TG-induced Ca2+ entry. We propose that STIM1, a ubiquitously expressed protein that is conserved from Drosophila to mammalian cells, plays an essential role in SOC influx and may be a common component of SOC and CRAC channels

Functional Coupling of Ca2+ Channels to Ryanodine Receptors at Presynaptic Terminals: Amplification of Exocytosis and Plasticity

Ca2+-induced Ca2+ release (CICR) enhances a variety of cellular Ca2+ signaling and functions. How CICR affects impulse-evoked transmitter release is unknown. At frog motor nerve terminals, repetitive Ca2+ entries slowly prime and subsequently activate the mechanism of CICR via ryanodine receptors and asynchronous exocytosis of transmitters. Further Ca2+ entry inactivates the CICR mechanism and the absence of Ca2+ entry for >1 min results in its slow depriming. We now report here that the activation of this unique CICR markedly enhances impulse-evoked exocytosis of transmitter. The conditioning nerve stimulation (10–20 Hz, 2–10 min) that primes the CICR mechanism produced the marked enhancement of the amplitude and quantal content of end-plate potentials (EPPs) that decayed double exponentially with time constants of 1.85 and 10 min. The enhancement was blocked by inhibitors of ryanodine receptors and was accompanied by a slight prolongation of the peak times of EPP and the end-plate currents estimated from deconvolution of EPP. The conditioning nerve stimulation also enhanced single impulse- and tetanus-induced rises in intracellular Ca2+ in the terminals with little change in time course. There was no change in the rate of growth of the amplitudes of EPPs in a short train after the conditioning stimulation. On the other hand, the augmentation and potentiation of EPP were enhanced, and then decreased in parallel with changes in intraterminal Ca2+ during repetition of tetani. The results suggest that ryanodine receptors exist close to voltage-gated Ca2+ channels in the presynaptic terminals and amplify the impulse-evoked exocytosis and its plasticity via CICR after Ca2+-dependent priming

Quantum Tunneling in Nuclear Fusion

Recent theoretical advances in the study of heavy ion fusion reactions below the Coulomb barrier are reviewed. Particular emphasis is given to new ways of analyzing data, such as studying barrier distributions; new approaches to channel coupling, such as the path integral and Green function formalisms; and alternative methods to describe nuclear structure effects, such as those using the Interacting Boson Model. The roles of nucleon transfer, asymmetry effects, higher-order couplings, and shape-phase transitions are elucidated. The current status of the fusion of unstable nuclei and very massive systems are briefly discussed.Comment: To appear in the January 1998 issue of Reviews of Modern Physics. 13 Figures (postscript file for Figure 6 is not available; a hard copy can be requested from the authors). Full text and figures are also available at http://nucth.physics.wisc.edu/preprints

Optical model potentials involving loosely bound p-shell nuclei around 10 MeV/A

We present the results of a search for optical model potentials for use in the description of elastic scattering and transfer reactions involving stable and radioactive p-shell nuclei. This was done in connection with our program to use transfer reactions to obtain data for nuclear astrophysics, in particular for the determination of the astrophysical S_17 factor for 7Be(p,\gamma)8B using two (7Be,8B) proton transfer reactions. Elastic scattering was measured using 7Li, 10B, 13C and 14N projectiles on 9Be and 13C targets at or about E/A=10 MeV/nucleon. Woods-Saxon type optical model potentials were extracted and are compared with potentials obtained from a microscopic double folding model. We use these results to find optical model potentials for unstable nuclei with emphasis on the reliability of the description they provide for peripheral proton transfer reactions. We discuss the uncertainty introduced by the procedure in the prediction of the DWBA cross sections for the (7Be,8B) reactions used in extracting the astrophysical factor S_17(0).Comment: 16 pages, LaTEX file, 9 figures (PostScript files

Experimental Search for Solar Axions

A new technique has been used to search for solar axions using a single crystal germanium detector. It exploits the coherent conversion of axions into photons when their angle of incidence satisfies a Bragg condition with a crystalline plane. The analysis of approximately 1.94 kg.yr of data from the 1-kg DEMOS detector in Sierra Grande, Argentina, yields a new laboratory bound on axion-photon coupling of g_{a,\gamma\gamma}<2.7\times 10^{-9} GeV^{-1} independent of axion mass up to \sim 1 keV

Potential improvements in global carbon flux estimates from a network of laser heterodyne radiometer measurements of column carbon dioxide

We present observing system simulation experiments (OSSEs) to evaluate the impact of a proposed network of ground-based miniaturized laser heterodyne radiometer (mini-LHR) instruments that measure atmospheric column-averaged carbon dioxide (XCO2) with a 1 ppm precision. A particular strength of this passive measurement approach is its insensitivity to clouds and aerosols due to its direct sun pointing and narrow field of view (0.2). Developed at the NASA Goddard Space Flight Center (GSFC), these portable, low-cost mini-LHR instruments were designed to operate in tandem with the sun photometers used by the AErosol RObotic NETwork (AERONET). This partnership allows us to leverage the existing framework of AERONET's global ground network of more than 500 sites as well as providing simultaneous measurements of aerosols that are known to be a major source of error in retrievals of XCO2 from passive nadir-viewing satellite observations. We show, using the global 3-D GEOS-Chem chemistry transport model, that a deployment of 50 mini-LHRs at strategic (but not optimized) AERONET sites significantly improves our knowledge of global and regional land-based CO2 fluxes. This improvement varies seasonally and ranges 58%81% over southern lands, 47%76% over tropical lands, 71%92% over northern lands, and 64%91% globally. We also show significant added value from combining mini-LHR instruments with the existing ground-based NOAA flask network. Collectively, these data result in improved a posteriori CO2 flux estimates on spatial scales of 10 km2, especially over North America and Europe, where the ground-based networks are densest. Our studies suggest that the mini-LHR network could also play a substantive role in reducing carbon flux uncertainty in Arctic and tropical systems by filling in geographical gaps in measurements left by ground-based networks and space-based observations. A realized network would also provide necessary data for the quinquennial global stock takes that form part of the Paris Agreement

Modulation of γ-Secretase Reduces β-Amyloid Deposition in a Transgenic Mouse Model of Alzheimer's Disease

SummaryAlzheimer's disease (AD) is characterized pathologically by the abundance of senile plaques and neurofibrillary tangles in the brain. We synthesized over 1200 novel gamma-secretase modulator (GSM) compounds that reduced Aβ42 levels without inhibiting epsilon-site cleavage of APP and Notch, the generation of the APP and Notch intracellular domains, respectively. These compounds also reduced Aβ40 levels while concomitantly elevating levels of Aβ38 and Aβ37. Immobilization of a potent GSM onto an agarose matrix quantitatively recovered Pen-2 and to a lesser degree PS-1 NTFs from cellular extracts. Moreover, oral administration (once daily) of another potent GSM to Tg 2576 transgenic AD mice displayed dose-responsive lowering of plasma and brain Aβ42; chronic daily administration led to significant reductions in both diffuse and neuritic plaques. These effects were observed in the absence of Notch-related changes (e.g., intestinal proliferation of goblet cells), which are commonly associated with repeated exposure to functional gamma-secretase inhibitors (GSIs)