Inflammation, neurodegeneration and protein aggregation in the retina as ocular biomarkers for Alzheimer’s Disease in the 3xTg-AD mouse model

Alzheimer's disease (AD) is the most common cause of dementia in the elderly. In the pathogenesis of AD a pivotal role is played by two neurotoxic proteins that aggregate and accumulate in the central nervous system: amyloid beta and hyper-phosphorylated tau. Accumulation of extracellular amyloid beta plaques and intracellular hyper-phosphorylated tau tangles, and consequent neuronal loss begins 10-15 years before any cognitive impairment. In addition to cognitive and behavioral deficits, sensorial abnormalities have been described in AD patients and in some AD transgenic mouse models. Retina can be considered a simple model of the brain, as some pathological changes and therapeutic strategies from the brain may be observed or applicable to the retina. Here we propose new retinal biomarkers that could anticipate the AD diagnosis and help the beginning and the follow-up of possible future treatments. We analyzed retinal tissue of triple-transgenic AD mouse model (3xTg-AD) for the presence of pathological hallmarks during disease progression. We found the presence of amyloid beta plaques, tau tangles, neurodegeneration, and astrogliosis in the retinal ganglion cell layer of 3xTg-AD mice, already at pre-symptomatic stage. Moreover, retinal microglia in pre-symptomatic mice showed a ramified, anti-inflammatory phenotype which, during disease progression, switches to a pro-inflammatory, less ramified one, becoming neurotoxic. We hypothesize retina as a window through which monitor AD-related neurodegeneration process

Gingival hyperplasia around dental implants in jaws reconstructed with free vascularized flaps: a case report series

Free vascularized flaps are the gold standard for reconstruction of the facial skeleton after surgical ablation of pathologies or when important atrophy of the jaws exists. A frequent problem seen during prosthetic rehabilitation after reconstruction with free vascularized flaps is the onset of hyperplastic granulomatous reactive tissue around the prosthetic abutment of the implant. The features of this phenomenon seem to be directly related to the characteristics of the periimplant tissue and of the manufacturing materials of the prosthesis and abutments. This complication can be seen quite often; we found it in 7 of 40 patients (17.5%). It does not seem to significantly affect the survival rate of implants. The aim of the study was to analyze the behavior of such lesions and to suggest our clinical approach with the management of these kinds of patients and complications. To remove gingival hyperplasia, we used either a traditional cold scalpel or an electric cautery or laser. We had good results using these tools. The onset of this phenomenon was not influenced by either the kind of implant and free flaps used or by the local conditions of the patients (such as radiotherapy). The number of recurrences was highly influenced by the oral hygiene of the patients

Light-meson leptonic decay rates in lattice QCD+QED

The leading electromagnetic (e.m.) and strong isospin-breaking corrections to the $\pi^+ \to \mu^+ \nu[\gamma]$ and $K^+ \to \mu^+ \nu[\gamma]$ leptonic decay rates are evaluated for the first time on the lattice. The results are obtained using gauge ensembles produced by the European Twisted Mass Collaboration with $N_f = 2 + 1 + 1$ dynamical quarks. The relative leading-order e.m.~and strong isospin-breaking corrections to the decay rates are 1.53(19)\% for $\pi_{\mu 2}$ decays and 0.24(10)\% for $K_{\mu 2}$ decays. Using the experimental values of the $\pi_{\mu 2}$ and $K_{\mu 2}$ decay rates and updated lattice QCD results for the pion and kaon decay constants in isosymmetric QCD, we find that the Cabibbo-Kobayashi-Maskawa matrix element $| V_{us}| = 0.22538(46)$, reducing by a factor of about $1.8$ the corresponding uncertainty in the Particle Data Group review. Our calculation of $|V_{us}|$ allows also an accurate determination of the first-row CKM unitarity relation $| V_{ud}|^2 + | V_{us}|^2 + | V_{ub}|^2 = 0.99988(46)$. Theoretical developments in this paper include a detailed discussion of how QCD can be defined in the full QCD+QED theory and an improved renormalisation procedure in which the bare lattice operators are renormalised non-perturbatively into the (modified) Regularization Independent Momentum subtraction scheme and subsequently matched perturbatively at $O(\alpha_{em}\alpha_s(M_W))$ into the W-regularisation scheme appropriate for these calculations.Comment: 63 pages, 10 figures and 2 tables. Version matches the published pape

The Schwinger Model on the lattice in the Microcanonical Fermionic Average approach

The Microcanonical Fermionic Average method has been used so far in the context of lattice models with phase transitions at finite coupling. To test its applicability to Asymptotically Free theories, we have implemented it in QED$_2$, \it i.e.\rm the Schwinger Model. We exploit the possibility, intrinsic to this method, of studying the whole $\beta, m$ plane at negligible computer cost, to follow constant physics trajectories and measure the $m \to 0$ limit of the chiral condensate. We recover the continuum result within 3 decimal places.Comment: TeX file, 7 pages + 3 figures in Postscrip

Efficacy of methylergometrine during the early puerperium: a randomized double-blind placebo-controlled clinical trial

OBJECTIVE: To determine if oral methylergometrine administration during the first 10 d following spontaneous vaginal delivery has any beneficial effect on the increase of hemoglobin levels. METHODS: This was a parallel group double-blind placebo-controlled clinical trial conducted at single center university hospital in Italy. Participants were puerperal women, who delivered singleton gestation with spontaneous vaginal delivery at term. Participants were randomized into a 1:1 ratio to receive either 0.125 mg methylergometrine per os twice a day or placebo for 10 d. Hemoglobin levels were recorded on the day of delivery and after 10 d. The primary outcome was the variation in hemoglobin levels between the first and the 10th day of treatment. RESULTS: From December 2012 to October 2015, 220 agreed to take part in the study, underwent randomization, and were enrolled and followed-up. Of the randomized women, 110 (50%) were randomized to the methylergometrine group and 110 (50%) to the placebo group. No women were excluded after randomization or lost to follow-up (100%). We found no significant difference in the median variation of hemoglobin levels between the intervention and the placebo group Conclusions: The use of 10 d oral methylergometrine in puerperal women was not associated with any benefit in the variation of hemoglobin levels from delivery to 10 d after delivery. Key Message Methylergometrine in puerperal women was not associated with any benefit

Finite Density QCD: a New Approach

We introduce a new approach to analyze the phase diagram of QCD at finite chemical potential and temperature, test it in the Gross-Neveu model at finite baryon density, and apply it to the study of the chemical potential-temperature phase diagram of QCD with four degenerate flavors of Kogut-Susskind type.Comment: 21 pages, 9 figures. Some comments and references adde

Management of a Complex Case during COVID-19 Time Using One-day Digital Dentistry: A Case Report

Aim and objective: The aim of the present case report is to describe the digital management of an implant prosthetic rehabilitation performed by the use of different digital technologies, which allowed to successfully perform in 1 day both the surgical and the prosthetical stages with a minimally invasive approach and a high standard of care. Background: Coronavirus disease-2019 (COVID-19) pandemic is affecting dental everyday practice. Clinicians have to reduce the number of patients per day and the time they spend in the dental office. Minimally invasive and digital approaches, with less possible exposure and interaction, are suggested to reduce the risk of infection. Case description: The failure of a short-span implant prosthetic rehabilitation combined with pain and mobility of the involved teeth was the main complaint reported by a 78-year-old male patient, who asked an urgent appointment to solve the problem. An intraoral scanner allowed the clinician to immediately take a preliminary digital impression of the arch to be treated. The resulting 3D files were sent by e-mail to the dental technician who provided a digital wax-up for the computerized workflow. Computer-aided implantology (CAI) performed using an in-office cone-beam computed tomography (CBCT) allowed clinician to guide the surgical approach in a prosthetic manner. Such an integration inside a well-defined workflow was the key for a successful and rapid treatment. Conclusion: By using new innovative digital technology, the treatment was completed in 1 day, reducing the risk of COVID-19 by limiting the number of appointments and reducing contacts in confined environments like the dental office and public transportations. It also helped to reduce materials production and people movement in the treatment of dental emergency. Clinical significance: The possibility of performing an effective treatment saving time by using efficient technology and a minimally invasive procedure highlights the importance of digital planning in order to optimize every single step of the treatment. Digital workflow reduces also the movement of potentially infected materials from the office to the dental laboratory