1 research outputs found
Biocompounds recovery from Spirulina by conventional and ohmic heating methodologies: chemical and biological properties
Extracting the totality of bio-compounds with industrial interest from Cyanobacterium is often prevented by
the intrinsic rigidity of its cell wall. In this sense, the present study focuses on evaluating the influence thermal
batch extraction (conventional extraction technologies) and ohmic heating (OH) assisted extraction
(considered a greener alternative technology) in blue green microalgae Arthrospira platensis (Spirulina) cell
disruption for bioactive fractions recovery.
The proximal composition of Spirulina was initially determined. The maximum protein content (i.e., CPhycocyanin),
total carbohydrates (TC) and total phenolic compounds (TPC) extracted in water at different
times (30-120 min) and temperatures (30-51 ºC) was quantified after the conventional and OH- assisted
extraction. The freeze-thawing process was used as control. The antioxidant activity (i.e., FRAP and DPPH
assays) of the obtained extracts was assessed.
Results showed that with the freeze-thawing process, traditionally used for the recovery of bio- compounds
from Spirulina, the concentration of C-phycocyanin was approx. 42 mg/g of Spirulina, 26 mgGlcE/g Spirulina
of TC and 9 mgGAE/g Spirulina of TPC. Using OH-assisted extraction, the maximum of C-Phycocyanin
content obtained was 45 mg/g of Spirulina (obtained at 37 ºC, 30 min), the maximum carbohydrates content
was 40 mgGlcE/g Spirulina and the maximum TPC was 10 mgGAE/g Spirulina. On the other hand, using
conventional thermal treatment it can be observed that, under the same conditions, the bioactive compounds
recovery decreased to 35 mg/g, 20 mgGlcE/g Spirulina for C-phycocyanin concentration and TC (p<0.05),
respectively. The concentration in phenolic compounds is not so affected, but even so the ohmic heating
potentiates the extraction of these secondary metabolites.
The antioxidant activity of the extracts there was not different between conventional treatments andOH.
Thus, the results indicated that OH is a good alternative to conventional methods aiming at the extraction of
intracellular components with a decrease in processing time and energy costs associated with the extraction
process, which together with an easy upscale make OH an interesting methodology for use in the industrial
production of microalgae colorants and bioactive supplements.info:eu-repo/semantics/publishedVersio