Start-up with or without inoculum? Analysis of an SMBR pilot plant.

This study analysed a submerged membrane bioreactor (SMBR) start-up with the purpose of determining the best conditions to carry it out. In order to do this, a hollow fibre membrane module was installed in a submerged configuration in a pilot aerobic reactor. The experiment was then divided in two phases, lasting 65 days each. During phase 1, the pilot plant was started-up without inoculum of activated sludge and no sludge, withdrawal was performed. Conversely, in phase 2, the MBR pilot plant was started-up with sludge inoculum and the sludge concentration was kept constant. In both phases, the volumetric loading rate applied to the pilot plant was kept constant. The authors analysed the difference in carbon removal performances, the evolution of floc sizes and the fouling rate in both phases. The results confirmed that MBRs can be quickly and easily started-up, but the initial start-up strategy can influence membrane fouling. More specifically, the carbon removal performances were similar in both phases, while the fouling rate increased faster during the start-up without inoculum, especially in terms of irreversible deposition of soluble compost on the membrane surface and into membrane pores.This study analysed a submerged membrane bioreactor (SMBR) start-up with the purpose of determining the best conditions to carry it out. In order to do this, a hollow fibre membrane module was installed in a submerged configuration in a pilot aerobic reactor. The experiment was then divided in two phases, lasting 65 days each. During phase 1, the pilot plant was started-up without inoculum of activated sludge and no sludge, withdrawal was performed. Conversely, in phase 2, the MBR pilot plant was started-up with sludge inoculum and the sludge concentration was kept constant. In both phases, the volumetric loading rate applied to the pilot plant was kept constant. The authors analysed the difference in carbon removal performances, the evolution of floc sizes and the fouling rate in both phases. The results confirmed that MBRs can be quickly and easily started-up, but the initial start-up strategy can influence membrane fouling. More specifically, the carbon removal performances were similar in both phases, while the fouling rate increased faster during the start-up without inoculum, especially in terms of irreversible deposition of soluble compost on the membrane surface and into membrane pores

The role of extracellular polymeric substances on aerobic granulation with stepwise increase of salinity

A granular sequencing batch reactor (GSBR) worked for 164 days to study the effect of salinity on aerobic granulation. The feeding had an organic loading rate (OLR) of 1.6 kg COD c5m 123 c5d 121 and a gradual increase of salinity (from 0.30 to 38 g NaCl 12 c5L 121) to promote a biological salt-adaptation. First aggregates (average diameter 48 0.4 mm) appeared after 14 days. Extracellular polymeric substances (EPSs) analyses revealed that proteins were mainly higher than polysaccharides, and microorganisms metabolized EPSs as additional carbon source, mostly in feast phase, to face the energy demand for salinity adaptation. No significant worsening of organic matter removal was observed. The initial decrease of nitrification (from 58% to 15%) and the subsequent increase (up to 25%), confirmed the acclimation of AOBs to saline environment, while the accumulation of nitrites suggested NOBs inhibition. The nitrogen removal initially decreased from 58% to 15%, due to the inhibitory effect of salinity, and subsequently increased up to 29% denoting a simultaneous nitrification\u2013denitrification. The dimensions of mature granules (higher than 1 mm) probably involved PAOs growth in the inner anaerobic layers. Nitrites caused a temporary deterioration of phosphorous removal (from 60% to almost zero), that increased up to 25% when nitrites were depleted

Cultivation of granular sludge with hypersaline oily wastewater

The time required to stabilise mature aerobic granules is rather variable. In addition, cultivation time and the structural characteristics of granules seem to be related to the nature of wastewater influent. Granular sludge has been used for the treatment of several industrial wastewaters, but nothing has been reported about wastewater characterized by the simultaneous presence of hydrocarbons and high chloride concentration. In this work, the authors analysed the granulation process and performance as well as the physical characteristics of aerobic granules in two Granular Sequencing Batch Airlift Reactors (GSBARs), fed with acetate-based synthetic wastewater in reactor 1 (R1) and with a mixture of real and simulated slop (R2). The results obtained in 100 days show that full granulation was achieved in both reactors. The granules in R2 developed more quickly, but they appeared slightly unstable and more susceptible to breaking. Despite high salt concentration, the efficiency of phosphorous and carbon removal was satisfactory. Low nitrification activity was observed in R1, confirming that a longer time is necessary to obtain the acclimation of autotrophic biomass in aerobic granules. In R2 the combined effect of salinity and hydrocarbons caused the inhibition of the autotrophic biomass, with the consequence that nitrification was absent. Hydrocarbons were initially removed by adsorption afterwards by biological degradation with a removal efficiency of over 90%

Preparazione di bifenoli naturali funzionalizzati con unità fotosensibili reversibili

Il lavoro di ricerca ha voluto investigare la funzionalizzazione di bifenili naturali al fine di una loro applicazione nel campo dei materiali organici fotosensibili reversibili, appartenenti alla classe delle molecole switches

Combining residue analysis of floors and ceramics for the study of activity areas at the Garum Shop at Pompeii

In this paper, we propose the application for the first time in the Mediterranean area of the combination of the study of chemical residues in floors and ceramics, with the aim of providing information about the activities carried out in archeological buildings. We chose the Garum Shop at Pompeii to test the method. In fact, due to the peculiarity of this archeological context, it provided an ideal case in which the activities performed are in part known, and the ceramic vessels recovered are still in situ. Floor samples were studied by means of spot tests developed in Mexico aimed at identifying the presence of phosphates, fatty acids, and protein residues, while the organic residues preserved in the ceramic matrix of amphorae, dolia, and other ceramic vessels were studied by gas chromatography coupled with mass spectrometry. Moreover, we integrated the data obtained with specific studies directed at better identifying the solid residues found inside two of the amphorae studied: botanical studies of fruit stones recovered in a Dressel 20 amphora and the characterization of the lime preserved in an African amphora. The research allowed for the identification of the traces of some of the activities performed, such as cooking and producing garum in the floors of the building, and the use and re-use of amphorae and dolia before the Vesuvian eruption

Does the feeding strategy enhance the aerobic granular sludge stability treating saline effluents?

The development and stability of aerobic granular sludge (AGS) was studied in two Sequencing Batch Reactors (SBRs) treating fish canning wastewater. R1 cycle comprised a fully aerobic reaction phase, while R2 cycle included a plug-flow anaerobic feeding/reaction followed by an aerobic reaction phase. The performance of the AGS reactors was compared treating the same effluents with variable salt concentrations (4.97e13.45 g NaCl/L) and organic loading rates (OLR, 1.80e6.65 kg CODs/(m3 $d)). Granulation process was faster in R2 (day 34) than in R1 (day 90), however the granular biomass formed in the fully aerobic configuration was more stable to the variable feeding composition. Thus, in R1 solid retention times (SRT), up to 15.2 days, longer than in R2, up to 5.8 days, were achieved. These long SRTs values helped the retention of nitrifying organisms and provoked the increase of the nitrogen removal efficiency to 80% in R1 while it was approximately of 40% in R2. However, the presence of an anaerobic feeding/reaction phase increased the organic matter removal efficiency in R2 (80e90%) which was higher than in R1 with a fully aerobic phase (75e85%). Furthermore, in R2 glycogen-accumulating organisms (GAOs) dominated inside the granules instead of phosphorous-accumulating organisms (PAOs), suggesting that GAOs resist better the stressful conditions of a variable and high-saline influent. In terms of AGS properties an anaerobic feeding/ reaction phase is not beneficial, however it enables the production of a better quality effluentThis research has been financed by the Spanish Government (AEI) through the projects GRANDSEA (CTM2014-55397-JIN) and TREASURE (CTQ2017-83225-C2-1-R), and by the European Commission (EU) through the LIFE project SEACAN (LIFE14ENV/ES/000852). The authors from the USC belong to the GRC ED431C 2017/29 and CRETUS (ED431E 2018/01). All these programs are co-financed by FEDER (EU) fundsS

Validation of a Commercial Loop-Mediated Isothermal Amplification (LAMP) Assay for the Rapid Detection of Anisakis spp. DNA in Processed Fish Products

Parasites belonging to the Anisakis genera are organisms of interest for human health because they are responsible for the Anisakiasis zoonosis, caused by the ingestion of raw or undercooked fish. Furthermore, several authors have reported this parasite to be a relevant inducer of acute or chronic allergic diseases. In this work, a rapid commercial system based on Loop-Mediated Isothermal Amplification (LAMP) was optimised and validated for the sensitive and rapid detection of Anisakis spp. DNA in processed fish products. The specificity and sensitivity of the LAMP assay for processed fish samples experimentally infected with Anisakis spp. larvae and DNA were determined. The LAMP system proposed in this study was able to give positive amplification for all the processed fish samples artificially contaminated with Anisakis spp., giving sensitivity values equal to 100%. Specificity tests provided no amplification for the Contracaecum, Pseudoterranova, or Hysterothylacium genera and uninfected samples. The limit of detection (LOD) of the LAMP assay proposed was 102 times lower than the real-time PCR method compared. To the best of our knowledge, this is the first report regarding the application of the LAMP assay for the detection of Anisakis spp. in processed fish products. The results obtained indicate that the LAMP assay validated in this work could be a reliable, easy-to-use, and convenient tool for the rapid detection of Anisakis DNA in fish product inspection

Residues of 165 pesticides in citrus fruits using LC-MS/MS: a study of the pesticides distribution from the peel to the pulp

Abstract A sensitive LC–ESI-MS/MS method was developed for the determination of 165 pesticides in 50 citrus fruit samples collected in Sicily. Moreover, an evaluation of pesticides levels in the citrus layers (peel, albedo, and pulp) was carried out. The method presented acceptable trueness, precision, and linearity with LOQ of 5 μg/kg. The results obtained showed a high frequency of fungicides class pesticides in all the citrus samples examined (>95%) with the highest concentrations in the peel (4468 µg/Kg). A significant difference of concentrations was found between the layers of the citrus fruits analysed (p < 0.05). In particular, the peel and albedo present higher pesticides significantly higher than the pulp. Our findings confirming the widespread use of these substances in citrus cultivation and suggesting the importance of pesticides analysis in all the citrus fruit layers separately, considering the different interactions between the physicochemical characteristics of the matrices and the pesticides

Dupilumab in the treatment of severe uncontrolled chronic rhinosinusitis with nasal polyps (CRSwNP): A multicentric observational Phase IV real-life study (DUPIREAL)

Background Chronic rhinosinusitis with nasal polyps (CRSwNP) is associated with significant morbidity and reduced health-related quality of life. Findings from clinical trials have demonstrated the effectiveness of dupilumab in CRSwNP, although real-world evidence is still limited. Methods This Phase IV real-life, observational, multicenter study assessed the effectiveness and safety of dupilumab in patients with severe uncontrolled CRSwNP (n = 648) over the first year of treatment. We collected data at baseline and after 1, 3, 6, 9, and 12 months of follow-up. We focused on nasal polyps score (NPS), symptoms, and olfactory function. We stratified outcomes by comorbidities, previous surgery, and adherence to intranasal corticosteroids, and examined the success rates based on current guidelines, as well as potential predictors of response at each timepoint. Results We observed a significant decrease in NPS from a median value of 6 (IQR 5–6) at baseline to 1.0 (IQR 0.0–2.0) at 12 months (p < .001), and a significant decrease in Sino-Nasal Outcomes Test-22 (SNOT-22) from a median score of 58 (IQR 49–70) at baseline to 11 (IQR 6–21; p < .001) at 12 months. Sniffin' Sticks scores showed a significant increase over 12 months (p < .001) compared to baseline. The results were unaffected by concomitant diseases, number of previous surgeries, and adherence to topical steroids, except for minor differences in rapidity of action. An excellent-moderate response was observed in 96.9% of patients at 12 months based on EPOS 2020 criteria. Conclusions Our findings from this large-scale real-life study support the effectiveness of dupilumab as an add-on therapy in patients with severe uncontrolled CRSwNP in reducing polyp size and improving the quality of life, severity of symptoms, nasal congestion, and smell

A machine-learning based bio-psycho-social model for the prediction of non-obstructive and obstructive coronary artery disease

Background: Mechanisms of myocardial ischemia in obstructive and non-obstructive coronary artery disease (CAD), and the interplay between clinical, functional, biological and psycho-social features, are still far to be fully elucidated. Objectives: To develop a machine-learning (ML) model for the supervised prediction of obstructive versus non-obstructive CAD. Methods: From the EVA study, we analysed adults hospitalized for IHD undergoing conventional coronary angiography (CCA). Non-obstructive CAD was defined by a stenosis < 50% in one or more vessels. Baseline clinical and psycho-socio-cultural characteristics were used for computing a Rockwood and Mitnitski frailty index, and a gender score according to GENESIS-PRAXY methodology. Serum concentration of inflammatory cytokines was measured with a multiplex flow cytometry assay. Through an XGBoost classifier combined with an explainable artificial intelligence tool (SHAP), we identified the most influential features in discriminating obstructive versus non-obstructive CAD. Results: Among the overall EVA cohort (n = 509), 311 individuals (mean age 67 ± 11 years, 38% females; 67% obstructive CAD) with complete data were analysed. The ML-based model (83% accuracy and 87% precision) showed that while obstructive CAD was associated with higher frailty index, older age and a cytokine signature characterized by IL-1β, IL-12p70 and IL-33, non-obstructive CAD was associated with a higher gender score (i.e., social characteristics traditionally ascribed to women) and with a cytokine signature characterized by IL-18, IL-8, IL-23. Conclusions: Integrating clinical, biological, and psycho-social features, we have optimized a sex- and gender-unbiased model that discriminates obstructive and non-obstructive CAD. Further mechanistic studies will shed light on the biological plausibility of these associations. Clinical trial registration: NCT02737982