Microbiological Quality and Adulteration of Pasteurized and Raw Milk Marketed in Dharan, Nepal

 This study was aimed to evaluate the quality of raw and pasteurized milk marketed in Dharan. Milk may be contaminated with pathogenic microorganisms and a mixture of several adulterants and such milk pose a risk to consumers. The study was carried out from September 2019 to January 2020. Collected samples were tested for adulterants (starch, formalin, neutralizer and table sugar) as well as microbial quality (Total Coliform count, Total Viable Count, Thermoduric Count, Escherichia. coli and Staphylococcus aureus) as per standard guideline. The adulterants starch, formalin and neutralizer were not detected in both raw and pasteurized milk. However, table sugar was present in 45% (9 out of 20) raw milk and 90% (18 out of 20) pasteurized milk. The average Total Viable Count, Total Coliform Count and Thermoduric Count of raw milk were, 59×105 CFU/ml, 14×104 CFU/ml and 5×103 CFU/ml respectively. Similarly, the average Total Viable Count, Total Coliform Count and Thermoduric Count of pasteurized milk were found to be 15×104 CFU/ml, 14×103CFU/ml and 4×103 CFU/ml respectively. E. coli was detected in 30% pasteurized milk whereas S. aureus was isolated from only 20%. Likewise, E.coli and S. aureus were found in 55% and 45% of raw milk respectively. The results of the study indicated that routine monitoring of dairy industries and raw milk vendors, awareness campaign and good hygienic practice should be promoted to upgrade the quality of raw and pasteurized milk.</jats:p

Food-borne bacterial pathogens in marketed raw meat of Dharan, eastern Nepal

Abstract Objectives This study aims to assess the bacteriological quality of marketed raw meat with a special emphasis on isolation of Escherichia coli, Salmonella spp., Shigella spp., Vibrio spp., Pseudomonas aeruginosa and Staphylococcus aureus in raw meat marketed in Dharan. Altogether 50 meat samples were collected from local markets of Dharan and transported to the microbiology laboratory at 4 °C. The meat samples were homogenized in a sterile glass homogenizer and the possible pathogens were isolated and identified by conventional microbiological techniques. Results The mean total viable count values were found having a mean count of 8.22 ± 0.14, 8.29 ± 0.17, 7.87 ± 0.18 and 7.92 ± 0.19 in terms of log10 CFU/g ± Standard Error for chicken, pork, buffalo, and goat meat respectively. Coliforms were found in 84% samples, S. aureus was found in 68% samples, Salmonella spp. in 34% samples, Shigella spp. in 6% samples, Vibrio spp. in only 3 samples and P. aeruginosa was isolated from 40% sample. Higher microbial load and presence of intestinal commensals E. coli, Salmonella spp., Shigella spp., Vibrio spp indicates that meat might be contaminated by the visceral content and consumers are at risk of getting a foodborne disease when eaten raw

Antibiotic resistance patterns of Staphylococcus aureus, Escherichia coli, Salmonella, Shigella and Vibrio isolated from chicken, pork, buffalo and goat meat in eastern Nepal

Abstract Objective Food-borne pathogens are a major cause of illnesses, death and expenses. Their occurrence in meat and other food is considered a global health problem. The burden of food-borne disease is increasing due to antimicrobial resistance which represents a greater risk of treatment failure. However, very little is known about the antibiotic resistance profile of food-borne pathogens in Nepal. This study was conducted to examine the antibiotic resistance profile of common food-borne bacterial pathogens isolated from raw meat sold in Nepal. A total of 83 meat samples were collected from the market and analyzed. Results The prevalence of Staphylococcus aureus, Escherichia coli, Salmonella, Shigella, and Vibrio were 68%, 53%, 35%, 6%, and 6% respectively. The resistance of Salmonella was most frequently observed to amoxicillin (100%), tetracycline (24%), chloramphenicol (11%), and nalidixic acid (11%). S. aureus was resistant to amoxicillin (100%) followed by tetracycline (63%), nalidixic acid (17%), and cefotaxime (13%) respectively. Vibrio isolates resisted amoxicillin (100%), tetracycline (40%) and chloramphenicol (20%). Shigella expressed the highest resistance to amoxicillin (100%), followed by chloramphenicol (80%), tetracycline (60%) and nalidixic acid (20%). E. coli exhibited the highest resistance to amoxicillin (100%), followed by tetracycline (93%), nalidixic acid (25%) and cefotaxime (19%). </jats:sec

Evaluation of physicochemical and microbiological quality of drinking water in the distribution system of Dharan, Nepal

Dharan has been facing drinking water-related problems for a very long time now and this research was conducted in order to determine whether the quality of water being distributed throughout the city was one of them. Hence, 31 samples were taken from the drinking water distribution system of Dharan in the spring of 2022 for evaluating the physicochemical and microbiological quality of drinking water being distributed across the sub-metropolitan city. Though public knowledge and adequate management of watershed and reservoir premises were insufficient, the physicochemical characteristics were determined to be within the National Drinking Water Quality Standards (NDWQS) for drinking water with temperatures ranging from 23.6 °C to 25.6 °C, pH 7.7 to 8.5, conductivity 38.2 to 38.7 µS/cm, Dissolved Oxygen (DO) 7.7 to 9.0 mg/L, Biological Oxygen Demand (BOD) 0.8 to 1.8 mg/L, chloride 29.82 to 34.08 mg/L, nitrite 10 mg/L and ammonia &lt;0.5 mg/L. However, the coliform bacteria levels were significant, with the highest TCC (Total Coliform Count) being 137 CFU/100mL, the highest FCC (Fecal Coliform Count) being 85 CFU/100mL and the highest TPC (Total Plate Count) being TMTC (Too Many To Count). The water was found to be unsafe to drink without disinfection treatments. It may be necessary to carry out treatment procedures like chlorination as advised by WHO (World Health Organization) as soon as possible while also taking into account the proper application of filtration techniques for distributing safe drinking water to the residents of Dharan.</jats:p

Intestinal Parasitic Infections and Associated Risk Factors Among School-going Children of Age 1-5 years in Dharan, Eastern Nepal

Intestinal parasitic infections have been a major public health burden of developing countries, especially in children. Higher prevalence has been reported among school children, mostly in rural areas of Nepal where water, toilets, hygiene, and sanitation facilities are insufficient. This cross-sectional study was carried out from April to September 2019 to determine the prevalence of intestinal parasitic infections among school-going children1-5 years of Dharan, Nepal, and to assess the associated risk factors. Stool samples were collected in a clean, dry, screw-capped, and wide-mouthed plastic container, kept in an icebox, and transported to the laboratory. Data relating to different risk factors were collected from the parents of 116 participants using a structured questionnaire. The parasites were identified by using the direct wet mount method and formal-ether concentration method. Pearson’s chi-square test was carried out to establish associations between dependent and independent variables using SPSS version 20, and the test considered a greater than &lt; 0.05% as statistically significant with a 95% confidence level. Out of the 116 stool samples, 9 (7.75%) tested positive for the parasitic infections in which 5 (55.5%) were protozoa and 4 (44.45%) were helminths. The prevalent parasites, were Entamoeba histolytica (23%), Hyamenolepis nana (22%), Giardia lamblia (11%), Hookworm (11%), Entamoeba coli (11%), Ascaris lumbricoides (11%), and Intestinal parasitic infections had a significant association with drinking water, bowel syndrome, bathing habit, toilet facility, and washing hands after toilets (p&lt;0.05). Lack of toilets, poor hygiene, and unsafe drinking water were the main risk factors. Improved hygienic practices, safe drinking water, and the use of latrines could lower the rate of parasitic infections.</jats:p

Dust Structure Nearby G229-03

The Sky View Virtual Observatory was used for the systematic search of dust structures within the far-infrared loop G229-03. The source (object) responsible for the formation of the cavity of interest was detected by the Set of Identifications, Measurements, and Bibliography for Astronomical Data (SIMBAD) database. The total mass of the loop was 8.50031 × 1029 kg which is about 0.425 times the mass of the Sun at a distance of 1300 pc. The size of the cavity was 3.67° × 3.6°, whereas its core size was 0.531° × 0.255° located at R.A. (J2000) = 7h10m0.8s and Dec.(J2000) =15h55m30s. The minimum and maximum temperatures were between 20.24 ± 1.16 K and 18.63 ± 1.96 K respectively. In the core region, the average temperature was 19.53 K, approximately equal to Gaussian center 19.267 K with an offset temperature of 0.4 K showing that the core region of the cavity is dynamically stable. The Far-infrared loop was found to be located within a 1° radius around the high-velocity cloud HVC oriented by 45° to the plane of the sky. The inclination angle of the core of the loop was greater than 60° whereas the inclination angle for the larger structure was 13.71°. The Gaussian distribution of temperature was well fitted with the center of 19.267 K which shows that the cavity was in thermal equilibrium and the outer region with offset temperature of about 35 K suggesting that the loop was dynamically unstable possibly due to high-velocity cloud</jats:p