Approximate Controllability of Delayed Fractional Stochastic Differential Systems with Mixed Noise and Impulsive Effects

We herein report a new class of impulsive fractional stochastic differential systems driven by mixed fractional Brownian motions with infinite delay and Hurst parameter $\hat{\cal H} \in ( 1/2, 1)$. Using fixed point techniques, a $q$-resolvent family, and fractional calculus, we discuss the existence of a piecewise continuous mild solution for the proposed system. Moreover, under appropriate conditions, we investigate the approximate controllability of the considered system. Finally, the main results are demonstrated with an illustrative example.Comment: Please cite this paper as follows: Hakkar, N.; Dhayal, R.; Debbouche, A.; Torres, D.F.M. Approximate Controllability of Delayed Fractional Stochastic Differential Systems with Mixed Noise and Impulsive Effects. Fractal Fract. 2023, 7, 104. https://doi.org/10.3390/fractalfract702010

Chitosan/polyaniline hybrid conducting biopolymer base impedimetric immunosensor to detect Ochratoxin-A

Chitosan (CS)–polyaniline (PANI) hybrid conducting biopolymer film was obtained on indium–tin-oxide (ITO) electrode using electrochemical polymerization process. Fourier transform infrared (FT-IR) spectra of PANI–CS had showed covalent and hydrogen binding between PANI and CS molecules. Electrochemical impedance spectroscopy (EIS) measurements had showed lowcharge transfer resistance (RCT) of PANI–CS and PANI. Successive rabbit antibody (IgGs) immobilizationonPANI–CS, CS and PANI matrixes surfacewere confirmed with FT-IR and EIS measurements. Ochratoxin-A (OTA) interaction with IgGs had increased RCT values and showed linear response up to 10 ng/mL OTA concentration in electrolyte. Relative change in RCT was higher in PANI–CS due to higher proportion of carboxylic and hydroxyl functionalities at PANI–CS matrix surfaces. The absolute sensitivity of PANI, CS, and PANI–CSwere 16±6, 22±9 and 53±8�mL/ng, respectively derived fromslope of linear response up to 10 ng/mL with 1 ng/mL minimum detection limit

Effect of foliar application of zinc and salicylic acid on growth, flowering and chemical constitute of African marigold cv. pusa narangi gainda (Targets erecta L.)

A field experiment on African marigold (Targets erecta L.) was conducted during winter season of 2014-15to study the foliar effect of Zn and SA of 20 treatment combinations having five concentrations of zinc (0.0, 0.25, 0.50, 0.75, and 1.0 %) and salicylic acid (0.0, 0.25, 0.50 and 1.0 mM/L).The treatmentZn4SA3 (Zinc 1% + Salicylic acid 1.0 mM/L) recorded the maximum plant height (77.41 cm), number of leaves per plant (314.10),earliest first flower bud appearance (39.78 days), maximum number of flowers per plant (62.33), maximum chlorophyll content (3.83mg/g) and maximum carotene content (3.07 mg/g)as compared to control where it was recorded minimum. These results are conclusive that foliar spraying with zinc 1.0% + salicylic acid 1.0 mM/L may positively increasedthe growth and flowering parametersof marigold

Salinity tolerance of cumin (Cuminum cyminum L.) genotypes during germination

A laboratory experiment was conducted to identify cumin (Cuminum cyminum L.) genotypes tolerant to salt at germination stage. Four salinity levels having electrical conductivity of 2.61 (control), 5.17 (low), S.10 (medium) and 12.15 (high) dSm-1 were tested. There is an increase in the suppression of seed germination with increase in salinity in all the genotypes tested. The genotype UC-20S showed maximum salt tolerance, followed by UC-209 and UC-2IS. &nbsp

Salinity tolerance of cumin (Cuminum cyminum L.) genotypes during germination

A laboratory experiment was conducted to identify cumin (Cuminum cyminum L.) genotypes tolerant to salt at germination stage. Four salinity levels having electrical conductivity of 2.61 (control), 5.17 (low), S.10 (medium) and 12.15 (high) dSm-1 were tested. There is an increase in the suppression of seed germination with increase in salinity in all the genotypes tested. The genotype UC-20S showed maximum salt tolerance, followed by UC-209 and UC-2IS. &nbsp

GPR120 (FFAR4) is preferentially expressed in pancreatic delta cells and regulates somatostatin secretion from murine islets of Langerhans

Open Access articleThe final publication is available at Springer via http://dx.doi.org/10.1007/s00125-014-3213-0AIMS/HYPOTHESIS: The NEFA-responsive G-protein coupled receptor 120 (GPR120) has been implicated in the regulation of inflammation, in the control of incretin secretion and as a predisposing factor influencing the development of type 2 diabetes by regulation of islet cell apoptosis. However, there is still considerable controversy about the tissue distribution of GPR120 and, in particular, it remains unclear which islet cell types express this molecule. In the present study, we have addressed this issue by constructing a Gpr120-knockout/β-galactosidase (LacZ) knock-in (KO/KI) mouse to examine the distribution and functional role of GPR120 in the endocrine pancreas. METHODS: A KO/KI mouse was generated in which exon 1 of the Gpr120 gene (also known as Ffar4) was replaced in frame by LacZ, thereby allowing for regulated expression of β-galactosidase under the control of the endogenous GPR120 promoter. The distribution of GPR120 was inferred from expression studies detecting β-galactosidase activity and protein production. Islet hormone secretion was measured from isolated mouse islets treated with selective GPR120 agonists. RESULTS: β-galactosidase activity was detected as a surrogate for GPR120 expression exclusively in a small population of islet endocrine cells located peripherally within the islet mantle. Immunofluorescence analysis revealed co-localisation with somatostatin suggesting that GPR120 is preferentially produced in islet delta cells. In confirmation of this, glucose-induced somatostatin secretion was inhibited by a range of selective GPR120 agonists. This response was lost in GPR120-knockout mice. CONCLUSIONS/INTERPRETATION: The results imply that GPR120 is selectively present within the delta cells of murine islets and that it regulates somatostatin secretion.BBSRC-CASE studentshipDiabetes U

Identification of high-performing antibodies for the reliable detection of Tau proteoforms by Western blotting and immunohistochemistry.

This is the final version. Available on open access from Springer via the DOI in this record. Data availability: All data generated or analyzed during this study are included in this published articleAntibodies are essential research tools whose performance directly impacts research conclusions and reproducibility. Owing to its central role in Alzheimer's disease and other dementias, hundreds of distinct antibody clones have been developed against the microtubule-associated protein Tau and its multiple proteoforms. Despite this breadth of offer, limited understanding of their performance and poor antibody selectivity have hindered research progress. Here, we validate a large panel of Tau antibodies by Western blot (79 reagents) and immunohistochemistry (35 reagents). We address the reagents' ability to detect the target proteoform, selectivity, the impact of protein phosphorylation on antibody binding and performance in human brain samples. While most antibodies detected Tau at high levels, many failed to detect it at lower, endogenous levels. By WB, non-selective binding to other proteins affected over half of the antibodies tested, with several cross-reacting with the related MAP2 protein, whereas the "oligomeric Tau" T22 antibody reacted with monomeric Tau by WB, thus calling into question its specificity to Tau oligomers. Despite the presumption that "total" Tau antibodies are agnostic to post-translational modifications, we found that phosphorylation partially inhibits binding for many such antibodies, including the popular Tau-5 clone. We further combine high-sensitivity reagents, mass-spectrometry proteomics and cDNA sequencing to demonstrate that presumptive Tau "knockout" human cells continue to express residual protein arising through exon skipping, providing evidence of previously unappreciated gene plasticity. Finally, probing of human brain samples with a large panel of antibodies revealed the presence of C-term-truncated versions of all main Tau brain isoforms in both control and tauopathy donors. Ultimately, we identify a validated panel of Tau antibodies that can be employed in Western blotting and/or immunohistochemistry to reliably detect even low levels of Tau expression with high selectivity. This work represents an extensive resource that will enable the re-interpretation of published data, improve reproducibility in Tau research, and overall accelerate scientific progress.Juvenile Diabetes Research FoundationWellcome TrustUCB BiopharmaMedical Research Council (MRC)University of OxfordResearch Englan