381 research outputs found

    COMPARISON OF ANTIBACTERIAL ACTIVITY OF HONEY AGAINST AEROBIC AND ANAEROBIC BACTERIA

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    Objective: To compare the antibacterial activity of honey against aerobic and anaerobic bacteria. Methods: Honey is extracted from the honey comb by trained persons. Antimicrobial activity of honey is performed by Agar Cup Diffusion technique for 3 bacteria Staphylococcus aureus, E. coli and Clostridium perfringens. Results: By performing the technique with proper guidance, it is observed that the Staphylococcus aureus specimen shows sensitivity to honey whereas the other two specimens Escherichia coli and clostridium perfringens doesn’t show any sensitivity to honey. Conclusion: Due to its vast antibacterial activity of honey, it can be used along with other antibiotics to increase its efficiency

    Electron paramagnetic resonance evidence for Jahn-Teller glasses

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    Single crystal E.P.R. studies of copper as a dopant in lithium potassium sulphate, lithium ammonium sulphate and lithium sodium sulphate have been carried out from room temperature down to 77K. The three Jahn-Teller (JT) systems behave very similarly to one another. The room temperature dynamic JT spectra with giso = 2.19 ± 0.01 and Aiso = ±(33 ± 4)× 10-4 cm-1 transform around 247 K to spectra characterized by randomly frozen-in axial strains with g = 2.4307 ± 0.0005, g = 2.083 ± 0.001, A = ±(116 ± 2) × 10-4 cm-1 and A = ±(14 ± 4) ×10-4 cm-1. We proposed that the low temperature phase (below 247 K) of each of these systems provides an example of a Jahn-Teller glass

    Growth and characterization of benzil single crystals using nanotranslation by the modified vertical Bridgman technique

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    Benzil single crystals have been grown by the modified vertical Bridgman technique using the double wall ampoule with nanotranslation for the first time. The characterization studies of benzil crystals grown by both single and double wall ampoules were analyzed. The grown benzil crystal was confirmed by single crystal and powder X-ray diffraction analyses. Fourier transform infrared analysis confirms the functional groups of the grown benzil. High resolution X-ray diffraction analysis indicates the crystalline perfection of the grown crystals. The UV-Vis-NIR studies show that the grown benzil crystals cutoff wavelength is around 434 nm. The green emission of the grown benzil was identified by photoluminescence studies. The thermal property of the grown benzil was studied by thermogravimetric and differential thermal analyses. The dielectric measurements of benzil crystals were carried out with different frequencies and temperatures and the results indicate an increase in dielectric and conductivity parameters with the increase of temperature at all frequencies. The second harmonic conversion efficiency of the grown benzil was determined

    ANALYSIS OF SULPHUR CONTENT IN COPRA

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    Agriculture is the largest economic sector in India. Coconut is one of the most demanded fruit amongst all. The dried coconut, copra is the main source of coconut oil. Naturally it contains 70% of moisture content and it is dried to about 7% for production of coconut oil. The sulphur is added as preservative which acts as anti-microbial agent for preventing bacteria, fungus etc. Sulphur is a toxic food preservative which restricts lung performance and leads to direct allergenic reactions. The survey of World Health Organisation says that 65% of asthmatic children are sensitive to sulphur and 75% of children exposed to sulphur exhibits changes in their behaviour. The sulphur fumigation over coconut affects human both externally and internally. Fumigation leads to cancer and environmental pollution. In order to prevent this devastating effect, copra is examined using image processing. The proposed idea is to identify the presence and percentage of sulphur region present in copra. The region of interest is segmented by method of superimposition thereby segmenting white layers in copra. The RGB colour features are extracted to differentiate the sulphur added copra from normal copra. The coconut is dried under 60? in a tray drier and shapes of copra decreases at regular interval of time are extracted using image processing. The decreasing percentage of shape features are measured to identify the sulphur added in the copra. The k-means clustering technique is used to discriminate the copra at different levels. The segmented patch area is measured to determine the percentage of sulphur present in copra. The percentage of sulphur over copra is divided into three levels (low sulphur added region, medium sulphur added region and high sulphur added region). The K-Nearest Neighbour classification is also used to classify the sulphur added copra at different levels. The proposed algorithm classifies the sulphur added copra at three different levels with 86% accuracy

    X-ray Structure of Gelatinase A Catalytic Domain Complexed with a Hydroxamate Inhibitor

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    Gelatinase A is a key enzyme in the family of matrix metalloproteinases (matrixins) that are involved in the degradation of the extracellular matrix. As this process is an integral part of tumour cell metastasis and angiogenesis, gelatinase is an important target for therapeutic intervention. The X-ray crystal structure of the gelatinase A catalytic domain (GaCD) complexed with batimastat (BB94), a hydroxamate inhibitor, shows an active site with a large S1\u27 specificity pocket. The structure is similar to previously solved structures of stromelysin catalytic domain (SCD) but with differences in VR1 and VR2, two surface-exposed loops on either side of the entrance to the active site. Comparison of GaCD with other members of the matrix metalloproteinase (MMP) family highlights the conservation of key secondary structural elements and the significant differences in the specificity pockets, knowledge of which should enhance our ability to design specific inhibitors for this important anticancer target
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