The Quest for Deeper Understanding in Interpretative Research: Hidden Meaning in Plain Sight

This paper contributes to the literature on qualitative methodology in a novel way, by being one of a handful of studies offering context and culture-bound insights of an interpretative analysis of meaning based on non-verbal communication from 49 semi-structured, face-to-face interviews. This paper is based on an interpretative phenomenological Ph.D. study, between 2017-2020, aiming to deepen our understanding of London-based Romanian migrant entrepreneurs' experiences of social inclusion through entrepreneurship. By leveraging the cultural insider positionality of the interviewer in this study, which granted direct access to this community and also valuable cultural understanding of participants’ non-verbal communication, seeking meaning within the untapped potential of around 93% non-verbal language, widely overlooked by qualitative researchers, has become an achievable research goal. By creating its own inventory of nonverbal communication codes, this paper uses interview extracts rich in nonverbal communication as illustrative examples to showcase their interpretative significance

Ethi(cs)quette of (Re)searching with E-friends: Clicking Towards a Social Media-driven Research Agenda

Social media increasingly shapes our professional and personal lives, leveraging its size, the potential for ubiquity, and real-time communication. Ranked the most popular social media platform by the number of subscribers, Facebook is increasingly gaining momentum as a research tool, mostly used to conduct surveys, adverts, and observation-driven research. However, Facebook’s potential for supporting consented qualitative research remains largely unexplored and deemed sometimes ethically questionable in the midst of ongoing debates around data protection rules and the ambiguity surrounding e-friendship meaning. This paper is based on an interpretative phenomenological Ph.D. study, between 2017-2020, aiming to deepen our understanding of London-based Romanian migrant entrepreneurs' experiences of social inclusion through entrepreneurship. This paper contributes to the literature on research methodology reflective practice of enabling ethical research, by outlining ethical implications of sampling via Facebook and when researching with e-friends as Facebook friends. It offers context-bound insights as guidance to researchers incorporating social media in their qualitative research The significance of this ethical research practice is discussed in terms of privacy, confidentiality, and informed consent as a cross point between GDPR regulatory framework, as universal research ethical framework, Facebook data privacy settings and the researcher’s reflective approach to mitigate ethical challenges experienced when recruiting Facebook e-friends

You scratch my back, and I scratch yours: Bartering for qualitative data

Recruiting research participants has been one of the significant challenges faced by qualitative researchers. Barter gained momentum during the Covid pandemic across a broad spectrum of professionals, including scholars searching to recruit research participants, despite being surrounded by ethical concerns of coercion or undue influence. This reflective paper created a barter reflective and ethical protocol showing how bartering created the entrepreneurial opportunity for 16 migrant entrepreneurs to exchange an average of 60 minutes of their time for participating in a qualitative interview with an average of 2.25 hrs (145 minutes) of business counselling and translation services delivered by the researcher. This paper contributes to the methodological practice of bartering. It argues that bartering is an ethical and efficient research practice in need of a code of ethics and protocol and should not be dismissed as ethically suspect until substantial evidence is brought forwar

Evaluation of a power driven residue manager for no-till drills

The main operational problem in direct drilling of paddy straw residue is the accumulation and wrapping of loose straw within/on the tines and frame of no-till drills and traction problems with the ground wheel. A residue management equipment (RME) is developed to cut and remove paddy straw away from furrow openers of the no-till drill. The equipment consisted of nine parts; each part consisted of two powered wheels, one wheel for cutting the residue and the other wheel for removing them away from no-till drill furrow openers. This equipment was attached with the no-till drill with inverted "T" type furrow opener and the experiments have been conducted to compare the no-till drill with RME and same no-till drill without RME. No-till drill with RME increased the fuel consumption and time required by 29.6 % and 13.14 %, respectively. Adding RME to the no-till drill decreased the amount of residue clogged by 33% and increased the percentage of cut hill from 14.9 to 63.7%. The average numbers of effective tiller, spike length and plant heights were more for no-till drill with RME. Furthermore, the grain yield was increased by 12.4% for fields with no-drill with RME

Effect of Different Levels of N and P on Ratoon Crop of Banana cv. Grand Naine

An investigation was carried out to study the effect of various levels of N and P on growth and yield of banana cv. Grand Naine in first ratoon crop at Punjab Agricultural University, Ludhiana. The treatments consisted of six levels of nitrogen at 150, 200 (in 4 and 5 splits), 250 (in 4 and 5 splits) and 300 g (in 5 splits) per plant as urea, phosphorus at 60 and 90 g per plant as single super phosphate. Application of N and P at the rate of 200 g N in 5 splits + 60 g P2O5 per plant to ratoon crop of banana cv. Grand Naine proved to be the best among all treatment combinations. This also resulted in maximum plant growth, early shooting and fruit maturity. In addition, the fruit yield per plant (18.9 kg) was maximum with the above mentioned treatment. Finger length increased with increase in dose of N from 150 g to 200 g per plant

Influence of Nitrogen and Phosphorus Fertilization on Fruiting and Yield Characteristics in Ratoon Crop of Banana (Musa spp. AAA) Cv. Grande Naine

In ratoon crop of banana cv. Grande Naine, date of shooting could be advanced by 35 days with application of 200g N in 5 splits + 60g P2O5 (86 days), compared to 300g N in 5 splits + 60g P2O5 (121 days). Subsequently, date of harvest also got advanced by 53 days, and fruits were harvested on 9th December in the same treatment. Higher dose of N fertilization delayed shooting and harvesting period, taking 121 days for shooting and 145 days from shooting to harvest in the treatment 300g N (5 split doses) + 90g P2O5. Various N and P treatments affected bunch weight and number of hands per bunch significantly. Although N and P combination-treatments had no significant effect on bunch weight or number of hands per bunch, application of 200g N in 5 splits and 60g P2O5 per plant gave maximum bunch weight (18.11kg) and number of hands per bunch (10.61). Minimum bunch weight (15.37kg) and the least number of hands per bunch (7.08) were obtained with 150g N in 5 splits + 90g P2O5. Hand-weight (2.20kg), number of fingers per hand (19.75), and finger length (20.30cm) was highest with application of 200g N in 5 splits + 60g P2O5 per plant. Least hand-weight (1.64kg), number of fingers per hand (15.77), and finger-length (17.92cm) was recorded with 150g N in 5 splits + 90g P2O5. Bunch weight, number of hands per bunch, hand-weight and number of fingers per hand too was affected significantly with sole application of nitrogen or phosphorus

Aegilops tauschii: A valuable source for karnal bunt resistance

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A candidate gene-based association study of introgressed pod shatter resistance in Brassica napus

267-276Yield losses at maturity due to unsynchronized pod shattering remain a major rapeseed breeding challenge. Variation for shatter resistance in the germplasm collections is inadequate for breeding manipulations. We have recently transferred resistance to pod shattering from Brassica carinata to Brassica napus. Introgression lines (ILs) were phenotyped for shatter resistance using the pendulum machine. Introgressive breeding was successful in enhancing rupture energy in the ILs, which varied from 1.8 to 7.2 milli Joules (mJ) for Environment 1 (E1) and 2.7 to 6.5 mJ for E2 while the corresponding values for natural B. napus ranged from 2.2-3.5 mJ (E1) and 2.2-4.3 mJ (E2), respectively. B. carinata had average rupture energy of 6.3 mJ (pooled over environments). On the basis of data averaged over two environments, I2 (6.3 mJ), I3 (5.2 mJ), I8 (5.6 mJ), I22 (5.1 mJ), I32 (5.2 mJ) and I41 (5.2 mJ) appeared very promising as germplasm resources for future breeding. Significant marker trait association between candidate gene NAC NAM (no apical meristem, Petunia), ATAF1/2 (Arabidopsis thaliana activating factor) and CUC2 (cup-shaped cotyledon, Arabidopsis) and rupture energy explained 19% of variation for the trait. IND3 (indehescent 3) also appeared to be associated with rupture energy under E1. These polymorphisms serve as encouraging candidates for developing molecular markers useful in marker-assisted deployment of introgressed shatter resistance

Prospective, randomized, double‐blind assessment of topical bakuchiol and retinol for facial photoageing

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/147746/1/bjd16918_am.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/147746/2/bjd16918.pd

Cellular Cytoskeleton Dynamics Modulates Non-Viral Gene Delivery through RhoGTPases

Although it is well accepted that the constituents of the cellular microenvironment modulate a myriad of cellular processes, including cell morphology, cytoskeletal dynamics and uptake pathways, the underlying mechanism of how these pathways influence non-viral gene transfer have not been studied. Transgene expression is increased on fibronectin (Fn) coated surfaces as a consequence of increased proliferation, cell spreading and active engagement of clathrin endocytosis pathway. RhoGTPases mediate the crosstalk between the cell and Fn, and regulate cellular processes involving filamentous actin, in-response to cellular interaction with Fn. Here the role of RhoGTPases specifically Rho, Rac and Cdc42 in modulation of non-viral gene transfer in mouse mesenchymal stem (mMSCs) plated in a fibronectin microenvironment was studied. More than 90% decrease in transgene expression was observed after inactivation of RhoGTPases using difficile toxin B (TcdB) and C3 transferase. Expression of dominant negative RhoA (RhoAT19N), Rac1(Rac1T17N) and Cdc42 (Cdc42T17N) also significantly reduced polyplex uptake and transgene expression. Interactions of cells with Fn lead to activation of RhoGTPases. However, further activation of RhoA, Rac1 and Cdc42 by expression of constitutively active genes (RhoAQ63L, Rac1Q61L and Cdc42Q61L) did not further enhance transgene expression in mMSCs, when plated on Fn. In contrast, activation of RhoA, Rac1 and Cdc42 by expression of constitutively active genes for cells plated on collagen I, which by itself did not increase RhoGTPase activation, resulted in enhanced transgene expression. Our study shows that RhoGTPases regulate internalization and effective intracellular processing of polyplexes that results in efficient gene transfer