Inhibition of autophagy by 3-MA enhances IL-24-induced apoptosis in human oral squamous cell carcinoma cells

Abstract Background Interleukin-24(IL-24), also referred to as melanoma differentiation-associated gene-7(mda-7), is a unique member of the IL-10 gene family, which displays nearly ubiquitous cancer-specific toxicity. The most notable feature of IL-24 is selectively induced growth suppression and apoptosis in various cancer cells, with no harmful effects toward normal cells. Autophagy is a self-protective mechanism in many kinds of tumor cells that respond to anticancer treatment. It is reported that autophagy inhibition could enhance the effects of many kinds of anticancer treatments, including gene therapy. However, whether IL-24 is effective to treat oral squamous cell carcinomas (OSCC) and if autophagy inhibition could improve the anticancer effect of IL-24 towards OSCC is has not been detected. Methods MTT assays were carried out to determine the cell proliferation; Transfection was used to gene transfer; Western Blot was performed to detect the protein level of LC3II, P62, Beclin 1, Cleaved caspase-3, β-Tubulin and β-actin; Apoptosis rates and cell cycle alteration were analyzed using flow cytometry; Autophagy induction was confirmed by MDC staining, GFP-LC3 staining and transmission electron microscopy. Amount of IL-24 in the culture medium was quantified by ELISA. Apoptosis in vivo was analyzed by TUNEL assay. HE staining was used to observe the morphology of the samples. Results In the present study, we proved that IL-24 have a novel anticancer effect towards KB cells and that autophagy inhibition could improve the anticancer effect of IL-24. IL-24 treated cells showed autophagy characteristics and autophagy inhibition by 3-methyladenine (3-MA) significantly enhanced IL-24-induced apoptosis. Similar results were obtained in the KB cells xenograft tumor model. Conclusions These results suggest that the combination of autophagy inhibitors and IL-24 based on the AdLTR2EF1α-mediated gene transfer could be a promising way to cure OSCC.http://deepblue.lib.umich.edu/bitstream/2027.42/113230/1/13046_2015_Article_211.pd

Dynamic Complexity of a Phytoplankton-Fish Model with the Impulsive Feedback Control by means of Poincaré Map

The phytoplankton-fish model for catching fish with impulsive feedback control is established in this paper. Firstly, the Poincaré map for the phytoplankton-fish model is defined, and the properties of monotonicity, continuity, differentiability, and fixed point of Poincaré map are analyzed. In particular, the continuous and discontinuous properties of Poincaré map under different conditions are discussed. Secondly, we conduct the analysis of the necessary and sufficient conditions for the existence, uniqueness, and global stability of the order-1 periodic solution of the phytoplankton-fish model and obtain the sufficient conditions for the existence of the order-kk≥2 periodic solution of the system. Numerical simulation shows the correctness of our results which show that phytoplankton and fish with the impulsive feedback control can live stably under certain conditions, and the results have certain reference value for the dynamic change of phytoplankton in aquatic ecosystems

HMM-Based Method for Aircraft Environmental Control System Turbofan Rolling Bearing Fault Diagnosis

In response to the high-noise, nonlinear, and nonstationary characteristics of vibration signals from aircraft environmental control system (ECS) turbofan rolling bearings, this paper proposes a diagnostic method for the degree of ECS turbofan bearing faults based on the Hidden Markov Model (HMM). Experimental results demonstrate that HMM can accurately diagnose and predict faults in ECS turbofan rolling bearings. The HMM method enhances diagnostic accuracy, and its effectiveness and feasibility in fault diagnosis based on different rolling bearing fault instances are elaborated. By employing the HMM model to establish precise models from decomposed dynamic data, it successfully identifies faults such as the fracture of the bearing cage under biased load conditions, although its performance in recognizing overheating faults is suboptimal

Atmospheric-Window-Matching Hierarchical Broadband Infrared Absorber Realized by Lithography-Free Fabrication

An ultra-broadband selective absorber has been realized with a hierarchical structure through integrating vacuum impedance-matched structure, quarter wavelength structure, and gradient refractive index structure. Through optimizing the design parameters of the proposed hierarchical structure, an ultra-broadband infrared absorber covering the three major atmospheric windows (0.7–2.5, 3–5, and 8–14 μm) has been numerically and experimentally demonstrated. An overall absorption up to 80% covering all the three major atmospheric infrared windows and a ratio of the total absorptions within and beyond the windows as high as 5.88 has been achieved with the developed absorber. The high absorption and spectral selectivity of the absorber make it promising for sensitive broadband infrared spectroscopy detection. The proposed hierarchical structure also provides great design freedom with many tunable factors, making it convenient to extend the design for other applications. In addition, we developed a cost-effective lithography-free method for the fabrication of this structure. The design flexibility and fabrication convenience of this hierarchical structure render it suitable for the development of tailored selective broadband absorbers for targeted applications

Dynamic Change of Sedimental Microbial Community During Black Bloom-an In Situ Enclosure Simulation Study

Black bloom is a worldwide environmental problem. Sediment microbes play important roles in the process of black bloom. The dynamic change of sedimental microbial community and their potential link between taste and odor compounds during black bloom was investigated in an in situ black bloom enclosure simulation experiment. Through high-throughput sequencing and analysis, pronounced shifts of sedimental microbial community were observed on the 3rd and 7th day in the black bloom group. Microbes in Cyanobacteria, Verrucomicrobia, Planctomycetes, and Actinobacteria were obviously increased, while microbes from the phyla OP8, Chloroflexi, and Acidobacteria were decreased significantly. RDA analysis revealed that the concentrations of chlorophyll a (Chla), total phosphorus (TP), and turbidity (NTU) in the water and the TP, TN concentrations in the sediment were the main environmental factors that affect the microbial community in the sediment. Correlation analysis revealed that microbesDechloromonassp.(OTU003567 and OTU000093),Desulfococcussp. (OTU000911),Chromatiaceae(OTU001222), andMethanosaetasp. (OTU004809) were positively correlated with the taste and odor substances in the sediment, such as dimethyl sulfide (DMS), beta-ionone, beta-cyclocitral and geosmin. The sedimental microbial community gradually recovered in the late phase of black bloom, indicating the stability and self-recovery ability of the sedimental microbial community during black bloom. Noteworthily, we observed many possible pathogens increased significantly during the black bloom, which alerts us to keep away from contaminated sediment when black bloom occurred

Comparative proteomics analysis of proteins expressed in the I-1 and I-2 internodes of strawberry stolons

Abstract Background Strawberries (Fragaria ananassa) reproduce asexually through stolons, which have strong tendencies to form adventitious roots at their second node. Understanding how the development of the proximal (I-1) and distal (I-2) internodes of stolons differ should facilitate nursery cultivation of strawberries. Results Herein, we compared the proteomic profiles of the strawberry stolon I-1 and I-2 internodes. Proteins extracted from the internodes were separated by two-dimensional gel electrophoresis, and 164 I-1 protein spots and 200 I-2 protein spots were examined further. Using mass spectrometry and database searches, 38 I-1 and 52 I-2 proteins were identified and categorized (8 and 10 groups, respectively) according to their cellular compartmentalization and functionality. Many of the identified proteins are enzymes necessary for carbohydrate metabolism and photosynthesis. Furthermore, identification of proteins that interact revealed that many of the I-2 proteins form a dynamic network during development. Finally, given our results, we present a mechanistic scheme for adventitious root formation of new clonal plants at the second node. Conclusions Comparative proteomic analysis of I-1 and I-2 proteins revealed that the ubiquitin-proteasome pathway and sugar-hormone pathways might be important during adventitious root formation at the second node of new clonal plants.</p