The effect of optimum dosage of Ovaprim injection on artificial spawning of Esox lucius

This project was conducted to explore the effect of optimum dosage of Ovaprim injection on the artificial spawning of Esox lucius. The research implemented by 4 treatments with 3 replicates for each one. Three female and six male brooders were injected in each replicate. The animals in 1, 2 and 3 treatments were injected by 10, 20 and 30 µg/kg BW, respectively, and 4 the treatment was considered as the control being injected with 4 mg/kg BW pituitary gland extract. Average (±SE) weights were 1361±521, 1376±954, 1009±160 and 1100 ±422 g in 1, 2, 3 and 4 treatments in females, respectively. In addition, positive response percent to hormone injection were measured as 77.8 ±19.24, 88.9 ± 19.24, 55.5 ±50.91 and 55.5 ± 19.24 % in 1, 2, 3 and 4 treatments in female and 94.4 ± 9.58, 88.9 ±19.26, 83.3±28.86 and 88.9 ± 19.26 % in male brooders, respectively. However, there was no significant different between all treatments. Fertilization content (±SE) in one to four treatments measured as 87.1±10, 88.04±7.7, 83.9±5.2 and 72.4±19.7 %, respectively. No significant differences were found among pairwise treatments. Average (±SE) percentage of eyed eggs were 66.6±15.9 for treatment one, 61.2±22.3 in treatment two, 58.3±10.7 in treatment three, and 56.1±15.04 in treatment four, with no significant pairwise differences. The average (±SE) hatching eggs were measured as 27.41±19.8 in treatment one, 39.53±26.9 in treatment two, 95.18±5.6 in treatment three and 26.78±12.4 in treatment four, with no significant pairwise differences. Also, the best dosage injection of ovaprim hormone was 10 and 20 µg/kgBW

Determination of optimum dosage of Ovaprim injectionon artificial spawning efficiency of Esox lucius

This project was conducted to goal of optimum dosage determination of ovaprim injection to artificial spawning efficiency of Esox lucius. The research implemented by 4 treatments with 3 replicates for each ones. 3 female and 6 male brooders injected in each replicate. The animals in 1, 2 and 3 treatments injected by 10, 20 and 30 µg/kg BW, respectively, and 4th treatment as a control injected with 4 mg/kg BW pituitary gland extract. Average weight of brooders were 1361±521, 1376±954, 1009±160 and 1100 ±422 g in 1, 2, 3 and 4 treatments in females and 689±145, 734±197, 547±118 and 794±238 g in males, respectively. In addition, positive response percent to hormone injection were measured 77.8 ±19.24 , 88.9 ± 19.24 , 55.5 ±50.91 and 55.5 ± 19.24 % in 1, 2, 3 and 4 treatments in female and 94.4 ± 9.58, 88.9 ±19.26 , 83.3±28.86 and 88.9 ± 19.26 % in male brooders, respectively, but there was no significant different between all of treatments (p<0.05). Incubation period from fertilization till hatching step in 7 to 15 ˚C was 5 to 10 days with average of 7±1.5 days. Fertilization content was in 1 to 4 treatments measured 87.1±10, 88.04±7.7, 83.9±5.2 and 72.4±19.7 %, respectively and also the treatments didn’t show any different significantly together (p<0.05). Average percentage of eyed eggs 66.6±15.9 in treat 1, 61.2±22.3 in treat 2, 58.3±10.7 in treat 3 and 56.1±15.04 in treat 4, without any significant different between of them (p<0.05). Hatching of eggs mean were measured 27.41±19.8 in treat 1, 39.53±26.9 in treat 2, 95.18±5.6 in treat 3 and 26.78±12.4 in treat 4, and significant different observed between of them too (p<0.05).In the other hand, mean percent of larvae with active feeding in these treatments were measured 18.77±14.6, 20.1±8.51, 55.6±11.6 and 14.51±7.72 as the treatments had significant different (p<0.05). Also, the best temperature and dosage injection of ovaprim hormone was 9 to 12.5 ˚C and 20µg/kg BW, respectively. The end of trial, from 103740 larvae introduced to earthen pond obtained 8000 fingerlings with weight of 2.68±0.6 g and length of 6.96±0.51 cm