A new artificial diet for the early weaning of seabass (<i>Dicentrarchus labrax</i>) larvae

The weaning of marine fish larvae is a critical step in larval rearing. The successful transition from live food to an artificial feed depends among others on the quality of the dry feed used (e.g. freshness of ingredients, digestibility, quality of the formulation) and the larvae themselves (e.g. age, behaviour towards artificial diets, development of the digestive tract, efficiency of certain metabolic pathways). The present study shows the results obtained with a new artificial diet formulated for the weaning phase of marine fish larvae (Dicentrarchus labrax) in commercial hatcheries

Eddy enhanced primary production accelerates bacterial growth in the Eastern Tropical North Atlantic

Mesoscale eddies play essential roles in modulating the ocean's physical, chemical, and biological properties. In cyclonic eddies (CE) nutrient upwelling can stimulate primary production by phytoplankton. Yet, how this locally enhanced autotrophic production affects heterotrophic bacterial activities (biomass production and respiration) and consequently the metabolic balance between the synthesis and the consumption of dissolved organic matter (DOM) remains largely unknown. To address this gap, we investigated the horizontal and vertical variability of phytoplankton and heterotrophic bacterial activity along ~900 km zonal corridor between the coast of Mauretania and the Cape Verde Islands in the eastern tropical North Atlantic (ETNA). We additionally collected samples from a CE along this transect at high spatial resolution. Our results show cascading effects of physical disturbances induced by a CE on phyto- and bacterioplankton biomass and metabolic activities. Specifically, the injection of nutrients into the sunlit surface resulted in enhanced autotrophic plankton abundance and activity as indicated by Chlorophyll a (Chl-a) concentration, DOM exudation, and primary productivity (PP). However, the detailed eddy survey revealed an uneven distribution of these parameters with, for example, the highest Chl-a concentrations and PP rates near and just beyond the CE’s periphery. The heterotrophic bacterial activity was similarly variable. Optode-based bacterial respiration (BR) and biomass production (BP) largely followed the trends of PP and Chl-a. Thus, a submesoscale spatial mosaic of heterotrophic bacterial abundance and activities occurred within the CE studied here that was closely related to variability in autotrophic production. This was supported by a significant positive correlation between concentrations of semi-labile organic carbon (SL-DOC; the sum of dissolved hydrolyzable amino acids and combined carbohydrates) and BR measurements. Bacterial growth efficiency (BP/(BR+BP)) was variable (1.4–10.5 %) within the CE and carbon exudation was not always sufficient to compensate the bacterial carbon demand (BR+BP; 28.3–114.5 %). We have additionally estimated the metabolic state in our samples, which showed that the CE carried a strong autotrophic signal (PP/(BR+BP) > 1). Overall, our results show that submesoscale (0–10 km) processes lead to highly variable metabolic activities of both phototrophic and heterotrophic microbes, which has implications for biogeochemical models estimating oceanic carbon fluxes. Additionally, we revealed that the CE not only traps and transports coastal nutrients and carbon to the open ocean but also stimulates phytoplankton growth generating freshly produced organic matter during westward propagation. This organic matter may fuel heterotrophic processes in the open ocean and may help to explain the often-observed net heterotrophic metabolic state of these environments

Eliminating Hepatitis C Virus From a Prevalent Kidney Transplant Recipient Population: A Single-Center Study in Belgium in the Direct-Acting Antivirals Era

Background: Direct-acting antivirals (DAAs) have revolutionized the treatment of hepatitis C virus (HCV) infection. Although previous studies have reported positive results with DAAs after kidney transplantation (KT), their impact on the prevalence of HCV viremia (HCVv) in prevalent kidney transplant recipients (KTRs) remains ill defined. Methods: We retrospectively reviewed the HCV status of all patients followed at Cliniques Universitaires Saint-Luc, Brussels, Belgium, outpatient KT clinic between January 2014 and December 2018. We collected the clinical features of KTRs treated with DAAs during this period and calculated the annual prevalence of HCVv over this period. Results: Out of 1451 KTRs, 22 (1.52%) had HCVv in 2014 to 2018. From 2014 to 2018, the annual prevalence of HCVv dropped from 1.97% to 0.43%, (P < .001). Fourteen KTRs were treated with DAAs a median of 197 months (range: 5-374) after KT, mostly (79%) in 2017 after reimbursement restrictions of DAAs for KTRs in Belgium were removed. DAA treatment was safe with a sustained virological response rate at 12 weeks after treatment (SVR12) of 93%. Two patients died 14 months (lymphoma, despite SVR12) and 7 months (hepatocarcinoma, no SVR12) after DAAs initiation, respectively. Among HCVv KTRs not treated with DAAs (n = 8), 2 lost their graft, 5 died, and 1 is initiating therapy. The current prevalence of HCVv in the cohort is 0.08%, with a single patient currently on treatment. Conclusion: Treatment with DAAs led to a dramatic decrease of HCVv prevalence in this KTR cohort. DAA use was safe and effective. Elimination of HCV is possible at KT clinics

Investigating the overhead of the REST protocol to reveal the potential for using cloud services for HPC storage

With the significant advances in Cloud Computing, it is inevitable to explore the usage of Cloud technology in HPC workflows. While many Cloud vendors offer to move complete HPC workloads into the Cloud, this is limited by the massive demand of computing power alongside storage resources typically required by I/O intensive HPC applications. It is widely believed that HPC hardware and software protocols like MPI yield superior performance and lower resource consumption compared to the HTTP transfer protocol used by RESTful Web Services that are prominent in Cloud execution and Cloud storage. With the advent of enhanced versions of HTTP, it is time to reevaluate the effective usage of cloud-based storage in HPC and their ability to cope with various types of data-intensive workloads. In this paper, we investigate the overhead of the REST protocol via HTTP compared to the HPC-native communication protocol MPI when storing and retrieving objects. Albeit we compare the MPI for a communication use case, we can still evaluate the impact of data communication and, therewith, the efficiency of data transfer for data access patterns. We accomplish this by modeling the impact of data transfer using measurable performance metrics. Hence, our contribution is the creation of a performance model based on hardware counters that provide an analytical representation of data transfer over current and future protocols. We validate this model by comparing the results obtained for REST and MPI on two different cluster systems, one equipped with Infiniband and one with Gigabit Ethernet. The evaluation shows that REST can be a viable, performant, and resource-efficient solution, in particular for accessing large files

Intermittent Surface Oxygenation Results in Similar Mitochondrial Protection and Maintenance of Aerobic Metabolism as Compared to Continuous Oxygenation during Hypothermic Machine Kidney Machine Perfusion

Short bubble and subsequent surface oxygenation is an innovative oxygenation technique and alternative for membrane oxygenation during hypothermic machine perfusion (HMP). The metabolic effect of the interruption of surface oxygenation for 4 h (mimicking organ transport) during HMP was compared to continuous surface and membrane oxygenation in a pig kidney ex situ preservation model. After 30 min of warm ischemia by vascular clamping, a kidney of a ±40 kg pig was procured and subsequently preserved according to one of the following groups: (1) 22-h HMP + intermittent surface oxygenation ( = 12); (2) 22-h HMP + continuous membrane oxygenation ( = 6); and (3) 22-h HMP + continuous surface oxygenation ( = 7). Brief perfusate O uploading before kidney perfusion was either obtained by direct bubble (groups 1, 3) or by membrane (group 2) oxygenation. Bubble oxygenation during minimum 15 min was as efficient as membrane oxygenation in achieving supraphysiological perfusate pO levels before kidney perfusion. Metabolic tissue analysis (i.e., lactate, succinate, ATP, NADH, and FMN) during and at the end of the preservation period demonstrated similar mitochondrial protection between all study groups. Short bubble and subsequent intermittent surface oxygenation of the perfusate of an HMP-kidney might be an effective and cheap preservation strategy to protect mitochondria, eliminating the need/costs of a membrane oxygenator and oxygen source during transport

Immunogenicity Profile of a 3.75-μg Hemagglutinin Pandemic rH5N1 Split Virion AS03A-Adjuvanted Vaccine in Elderly Persons: A Randomized Trial

Background. Elderly persons often experience a reduced immune response to influenza vaccination. We evaluated the usual dose of AS03A-adjuvanted H5N1 pandemic vaccine (3.75 μg hemagglutinin of A/Vietnam/1194/2004-like strain) compared with a double dose in an elderly population

Eddy Study to Understand Physical-Chemical-Biological Coupling and the Biological Carbon Pump as a Function of Eddy Type off West Africa, Cruise No. M160, 23.11.2019 - 20.12.2019, Mindelo (Cabo Verde) - Mindelo (Cabo Verde)

Cruise M160 is part of concerted MOSES/REEBUS Eddy Study featuring three major research expeditions (M156, M160, MSM104). It aims to develop both a qualitative and quantitative understanding of the role of physical-chemical-biological coupling in eddies for the biological pump. The study is part of the MOSES “Ocean Eddies” event chain, which follows three major hypotheses to be addressed by the MOSES/REEBUS field campaigns: (1) Mesoscale and sub-mesoscale eddies play an important role in transferring energy along the energy cascade from the large-scale circulation to dissipation at the molecular level. (2) Mesoscale and sub-mesoscale eddies are important drivers in determining onset, magnitude and characteristics of biological productivity in the ocean and contribute significantly to global primary production and particle export and transfer to the deep ocean. (3) Mesoscale and sub-mesoscale eddies are important for shaping extreme biogeochemical environments (e.g., pH, oxygen) in the oceans, thus acting as a source/sink function for greenhouse gases. In contrast to the other two legs, MOSES Eddy Study II during M160 did not include any benthic work but focused entirely on the pelagic dynamics within eddies. It accomplished a multi-disciplinary, multi-parameter and multi-platform study of two discrete cyclonic eddies in an unprecedented complexity. The pre-cruise search for discrete eddies suitable for detailed study during M160 had already started a few months prior to the cruise. Remote sensing data products (sea surface height, sea surface temperature, ocean color/chlorophyll a) were used in combination with eddy detection algorithms and numerical modelling to identify and track eddies in the entire eddy field off West Africa. In addition, 2 gliders and 1 waveglider had been set out from Mindelo/Cabo Verde for pre-cruise mapping of the potential working area north of the Cabo Verdean archipelago. At the start of M160, a few suitable eddies – mostly of cyclonic type – had been identified, some of which were outside the safe operation range of the motorglider plane. As technical problems delayed the flight operations, the first eddy (center at 14.5°N/25°W) for detailed study was chosen to the southwest of the island of Fogo. It was decided to carry out a first hydrographic survey there followed by the deployment of a suite of instruments (gliders, waveglider, floats, drifter short-term mooring). Such instrumented, we left this first eddy and transited – via a strong anticyclonic feature southwest of the island of Santiago – to the region northeast of the island of Sal, i.e. in the working range of the glider plane. During the transit, a full suite of underway measurements as well as CTD/RO section along 22°W (16°-18.5°N) were carried in search for sub-surface expressions of anticyclonic eddy features. In the northeast, we had identified the second strong cyclonic eddy (center at 18°N/22.5°W) which was chosen for detailed study starting with a complete hydrographic survey (ADCP, CTD/RO, other routine station work). After completion of the mesoscale work program, we identified a strong frontal region at the southwestern rim of the cyclonic eddy, which was chosen for the first sub-mesoscale study with aerial observation component. There, the first dye release experiment was carried out which consisted of the dye release itself followed by an intense multi-platforms study of the vertical and horizontal spreading of the initial dye streak. This work was METEOR-Berichte, Cruise M160, Mindelo – Mindelo, 23.11.2019 4 – 20.12.2019 supported and partly guided by aerial observation of the research motorglider Stemme, which was still somewhat compromised by technical issues and meteorological conditions (high cloud cover, Saharan dust event). Nevertheless, this first dye release experiment was successful and showed rapid movement of the dynamic meandering front. After completion of work on this second eddy and execution of a focused sampling program at the Cape Verde Ocean Observation, RV METEOR returned to the first eddy for continuation of the work started there in the beginning of the cruise. This was accompanied by a relocation of the airbase of Stemme from the international airport of Sal to the domestic airport of Fogo. The further execution of the eddy study at this first eddy, which again included a complete hydrographic survey followed by a mesoscale eddy study with dye release, was therefore possible with aerial observations providing important guidance for work on RV METEOR. Overall, M160 accomplished an extremely intense and complex work program with 212 instrument deployments during station work, 137 h of observation with towed instruments and a wide range of underway measurements throughout the cruise. Up to about 30 individually tracked platforms (Seadrones, glider, wavegliders, drifters, floats) were in the water at the same time providing unprecedented and orchestrated observation capabilities in an eddy. All planned work components were achieved and all working groups acquired the expected numbers of instrument deployments and sampling opportunities

Adaptative Strategy of Immunosuppressive Drugs Dosage Adjustments When Combined With Nirmatrelvir/Ritonavir in Solid Organ Transplant Recipients With COVID-19

Nirmatrelvir/ritonavir is a promising option for preventing severe COVID-19 in solid organ transplant recipients with SARS-CoV-2 infection. However, concerns have arisen regarding potential drug interactions with calcineurin inhibitors (CNI). This two-phase multicentre retrospective study, involving 113 patients on tacrolimus and 13 on cyclosporine A, aimed to assess the feasibility and outcomes of recommendations issued by The French societies of transplantation (SFT) and pharmacology (SFPT) for CNI management in this context. The study first evaluated adherence to recommendations, CNI exposure, and clinical outcomes. Notably, 96.5% of patients on tacrolimus adhered to the recommendations, maintaining stable tacrolimus trough concentrations (C0) during nirmatrelvir/ritonavir treatment. After reintroduction, most patients experienced increased C0, with 42.9% surpassing 15 ng/mL, including three patients exceeding 40 ng/mL. Similar trends were observed in cyclosporine A patients, with no COVID-19-related hospitalizations. Moreover, data from 22 patients were used to refine the reintroduction strategy. Modelling analyses suggested reintroducing tacrolimus at 50% of the initial dose on day 8, and then at 100% from day 9 as the optimal approach. In conclusion, the current strategy effectively maintains consistent tacrolimus exposure during nirmatrelvir/ritonavir treatment, and a stepwise reintroduction of tacrolimus may be better suited to the low CYP3A recovery