13 research outputs found
Specialized palliative care services are associated with improved short- and long-term caregiver outcomes
Vaginal Epithelial Cell-Derived S100 Alarmins Induced by Candida albicans via Pattern Recognition Receptor Interactions Are Sufficient but Not Necessary for the Acute Neutrophil Response during Experimental Vaginal Candidiasis
The axis of the receptor for advanced glycation endproducts in asthma and allergic airway disease
Dermatitis Herpetiformis-an Autoimmune Disease due to Cross-Reaction between Dietary Glutenin and Dermal Elastin?
Serum lipoproteins and cholesterol metabolism in two hypercholesterolaemic rabbit models
Anti-α-gliadin antibodies (AGA) in the serum of coeliac children and controls recognize an identical collection of linear epitopes of α-gliadin
Anti-gliadin antibodies can be found in the serum of patients with overt and subclinical coeliac disease, but also in that of some controls. The aim of the present study was to identify the linear epitopes of the α-gliadin molecule to which the humoral response is directed. Therefore, the IgG and IgA antibody reactivity against an overlapping set of synthetic peptides covering the entire sequence of α-gliadin was measured in the sera from patients with coeliac disease, from controls with elevated titres of anti-gliadin antibodies and from healthy children using an ELISA technique. The antibodies mainly recognize peptides derived from the N-terminal region of α-gliadin, containing the motif QPFXXQXPY. Reactivity was also detected against two other synthetic peptides, which do not contain this motif and represent a sequence encoded further to the C-terminal region of α-gliadin. Anti-gliadin antibodies in sera from patients with coeliac disease and from controls recognize the same linear epitopes. Thus, serological investigation of the specificity of these antibodies using a peptide ELISA does not allow discrimination between patients and controls