2017 update of the WSES guidelines for emergency repair of complicated abdominal wall hernias

Emergency repair of complicated abdominal wall hernias may be associated with worsen outcome and a significant rate of postoperative complications. There is no consensus on management of complicated abdominal hernias. The main matter of debate is about the use of mesh in case of intestinal resection and the type of mesh to be used. Wound infection is the most common complication encountered and represents an immense burden especially in the presence of a mesh. The recurrence rate is an important topic that influences the final outcome. A World Society of Emergency Surgery (WSES) Consensus Conference was held in Bergamo in July 2013 with the aim to define recommendations for emergency repair of abdominal wall hernias in adults. This document represents the executive summary of the consensus conference approved by a WSES expert panel. In 2016, the guidelines have been revised and updated according to the most recent available literature.Peer reviewe

2013 WSES guidelines for management of intra-abdominal infections

Peer reviewe

In-vitro activity of rifabutin against rifampicin-resistant Mycobacterium tuberculosis isolates with known rpoB mutations

WOS: 000222261100012PubMed ID: 15214882The relationship between resistance to rifampicin and rifabutin and genetic alterations in the rpoB gene of 41 rifampicin-resistant isolates of Mycobacterium tuberculosis was evaluated. Although 35 isolates with rifampicin MICs greater than or equal to 32 mg/L were also rifabutin-resistant, six isolates with rifampicin MICs of 2-16 mg/L were susceptible to rifabutin (MIC less than or equal to 0.5 mg/L). Mutations Asp516Val, Asp516Tyr, Leu533Pro and the double mutation Met515Ile and Leu533Pro influenced susceptibility to rifampicin, but not to rifabutin. All mutations at codons 531 and 526, except one isolate with a His526Cys mutation, correlated with resistance to both compounds

Molecular identification and DNA sequencing of Trichomonas vaginalis strains from agean region of Turkey

The aim of this study is to compare the presence of Trichomonas vaginalis (T. vaginalis) in symptomatic and asymptomatic women through microscopic examination, culture in Trypticase-Yeast Maltose (TYM) medium and PCR methods. In addition, T. vaginalis strains were analysed for genotyping with 18S rRNA-DNA and phylogenetic analysis. Axenized strains of T. vaginalis isolated from urine culture samples taken from symptomatic and asymptomatic women with clinical signs. Molecular characterization of the isolated strains of T. vaginalis was performed by using PCR. To evaluate molecular diagnosis and genotypic identification of T. vaginalis strains, 14 samples were analysed. Of the 14 samples, T. vaginalis was positive in 14 samples by microscopy, 6 in culture(TYM medium) and 14 by PCR, respectively. Although the sample size is very small, PCR was shown to be high sensitivity and specificity, and seems to be a promising diagnostic tool. 18S rRNA-DNA PCR results also confirmed with real time PCR method. In conclusion, it is considered that two strains of T. vaginalis isolated from samples, 5-TV1G and 13-TV1G, are subtypes of T. vaginalis as a result of 18S rRNA-DNA sequencing analysis. To best of our knowledge this is the first analysis of phylogenetic positions on T. vaginalis from Turkey. © 2017, Malaysian Society for Parasitology. All rights reserved

The effect of preoperative 5-fluorouracil on colonic healing: An experimental study

WOS: 000172705600026PubMed ID: 11813589Background/Aims: After curative resection for colorectal carcinoma there is a high recurrence rate and neoadjuvant chemotherapy may be useful in some patients. Very little is known about the effect of preoperative 5-fluorouracil on the healing of colon anastomosis. The aim of this study was to evaluate the effect of 5-fluorouracil on colonic healing when the time interval between the last injection and operation was shortened to 24 hours. Methodology: Thirty-six male Wistar rats with a median weight of 185g (range: 165-200g) were divided into three groups: 1) control group (n=12); 2) sham group (n=12) which received saline intraperitoneally, and 3) study group (n=12) which received 5-fluorouracil intraperitoneally (20mg/kg(-1)). All injections were given intraperitoneally for 5 days and the last dose was injected 24 hours before operation. Results: The mortality rate (22.7%) and anastomotic complications (29.4%) were increased in the 5-fluorouracil group, compared with the control or saline groups (P <0.05). The anastomotic bursting pressure in rats having 5-fluorouracil treatment (27mm Hg) was significantly lower from both the control (55mm Hg) and saline (84mm Hg) groups on postoperative day 3 (P <0.05). Both myeloperoxidase and hydroxyproline contents were also significantly lower than the other groups (P <0.05). Conclusions: Colonic healing was impaired and mortality rate was increased when intraperitoneal 5-fluorouracil treatment was repeated until 24 hours before operation

Molecular Identification and DNA Sequencing of Trichomonas vaginalis Strains from Agean region of Turkey

The aim of this study is to compare the presence of Trichomonas vaginalis (T. vaginalis) in symptomatic and asymptomatic women through microscopic examination, culture in Trypticase-Yeast Maltose (TYM) medium and PCR methods. In addition, T. vaginalis strains were analysed for genotyping with 18S rRNA-DNA and phylogenetic analysis. Axenized strains of T. vaginalis isolated from urine culture samples taken from symptomatic and asymptomatic women with clinical signs. Molecular characterization of the isolated strains of T. vaginalis was performed by using PCR. To evaluate molecular diagnosis and genotypic identification of T. vaginalis strains, 14 samples were analysed. Of the 14 samples, T. vaginalis was positive in 14 samples by microscopy, 6 in culture(TYM medium) and 14 by PCR, respectively. Although the sample size is very small, PCR was shown to be high sensitivity and specificity, and seems to be a promising diagnostic tool. 18S rRNA-DNA PCR results also confirmed with real time PCR method. In conclusion, it is considered that two strains of T. vaginalis isolated from samples, 5-TV1G and 13-TV1G, are subtypes of T. vaginalis as a result of 18S rRNA-DNA sequencing analysis. To best of our knowledge this is the first analysis of phylogenetic positions on T. vaginalis from Turkey