STUDY ON HPLC OF AROMATIC AMINO ACIDS AND OLIGO PEPTIDES USING MICROSPHERICAL CARBON PACKINGS : HPLC of Amino Acids and Oligo Peptides on Carbon Column

A microspherical carbon (MSC) as HPLC packings has been tested and its chromartographic propertier for the separation of aromatic amino acids and some oligo peptides have been studied in this paper. Theapparent characterization o packings were described by scanning electron microggraph and physical parameters. The mixture of aramatic amino acids can be separated satisfacterily on MSC column (7.8mm I. D. ×50mm) using acetonitrile eluate of the linear gradient, while the non-aromatic amino acids were not retained under the same conditions. The good results for the separation of some oligo peptides bearing aromatic structure can be obtained, while the other type of oligo peptides were not yet retained on MSC column unless the sample possess rather strong hydrophobicity. The high selective retention is a significant characteristic of MSC packing, and the chromatographic method described is useful for the separation of biochemical substances especially as some oligo peptides from the hydrolysis products of proteins or from the drinks. The separation machanism and exiting problem for MSC packings were also discussed preliminarily