An Investigation Of The Effects Of Inoculated And Intralymphatic Vaccinia Virus On Primary And Secondary Deposits Of Malignant Melanoma

A CAJM investigation of the Effects of Inoculated and Intralymphatic Vaccinia Virus on Primary and Secondary Deposits of Malignant Melanoma.The six patients included in this investigation were selected on the basis of histologically-proven malignant melanomas of the lower extremity, with either local intradermal metastases or regional lymph node involvement. If unvaccinated in the previous five years, vaccination was performed at least 14 days before using the virus therapeutically. Vaccinia virus was prepared from stock vacccine obtained from the South African Institute for Medical Research. Virus was inoculated on the Chorio-allantoic membrane of 12 day embryonated hens’ eggs in which growth was confluent on the third day. Virus was extracted in buffered saline, cleared and tested for sterility. The titre of the undiluted material was 107 pock forming units per ml. This was used at a dilution of 1:50 for both the superficial and deep lesions

An Investigation Of The Effects Of Inoculated And Intralymphatic Vaccinia Virus On Primary And Secondary Deposits Of Malignant Melanoma

The six patients included in this investigation were selected on the basis of histologically-proven malignant melanomas of the lower extremity, with either local intradermal metastases or regional lymph node involvement. If unvaccinated in the previous five years, vaccination was performed at least 14 days before using the virus therapeutically. Vaccinia virus was prepared from stock vacccine obtained from the South African Institute for Medical Research. Virus was inoculated on the Chorio-allantoic membrane of 12 day embryonated hens’ eggs in which growth was confluent on the third day. Virus was extracted in buffered saline, cleared and tested for sterility. The titre of the undiluted material was 107 pock forming units per ml. This was used at a dilution of 1:50 for both the superficial and deep lesions

Three Methods of Assessing Adolescent School-Level Experimentation of Tobacco Products

Three methods of use of estimate tobacco products experimentation were examined in nineteen schools (thirteen junior high schools and six high schools). Convergent and discriminant validity of measures of student experimentation of cigarettes and smokeless tobacco were assessed using Campbell and Fiske\u27s (1959) criteria to analyze a multitrait-multimethod correlation matrix produced by two traits (cigarettes and smokeless tobacco) and three methods (aggregated students\u27 self-report of tobacco experimentation, school personnel prevalence estimates of student tobacco experimentation, and school outdoor refuse evidence of tobacco products). The student self-report and the school staff estimate methods demonstrated good convergent validity. The refuse method showed convergent validity with the student self-reports of smokeless tobacco only. Evidence for discriminant validity was only suggestive. It appears that the school personnel method is the most useful and economic alternative for estimating school-level tobacco experimentation