3 research outputs found
Fluorescence Quenching at High Quencher Concentrations
Chemical reactions occurring in dense media at high reactant concentrations can be described by rate ââconstantsââ which are actually functions of concentration. We present a theoretical model in which this soâcalled rate constant âârenormalizationââ occurs for the specific case of fluorescence quenching in solution. We show that both the quenching and the excitation rate constants can become concentration dependent. We fit our theory to several sets of experimental dataâour own and some from the literatureâand show that excellent agreement is obtained by varying a single free parameter, namely, the efficiency with which a fluorophoreâquencher collision leads to a quench of the excited state