Using modern plant breeding to improve the nutritional and technological qualities of oil crops

The last few decades have seen huge advances in our understanding of plant biology and in the development of new technologies for the manipulation of crop plants. The application of relatively straightforward breeding and selection methods made possible the “Green Revolution” of the 1960s and 1970s that effectively doubled or trebled cereal production in much of the world and averted mass famine in Asia. During the 2000s, much attention has been focused on genomic approaches to plant breeding with the deployment of a new generation of technologies, such as marker-assisted selection, next-generation sequencing, transgenesis (genetic engineering or GM) and automatic mutagenesis/selection (TILLING, TargetIng Local Lesions IN Genomes). These methods are now being applied to a wide range of crops and have particularly good potential for oil crop improvement in terms of both overall food and non-food yield and nutritional and technical quality of the oils. Key targets include increasing overall oil yield and stability on a per seed or per fruit basis and very high oleic acid content in seed and fruit oils for both premium edible and oleochemical applications. Other more specialised targets include oils enriched in nutritionally desirable “fish oil”-like fatty acids, especially very long chain !-3 acids such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), or increased levels of lipidic vitamins such as carotenoids, tocopherols and tocotrienes. Progress in producing such oils in commercial crops has been good in recent years with several varieties being released or at advanced stages of development

Caleosin/Peroxygenases:multifunctional proteins in plants

BACKGROUND: Caleosin/peroxygenases (CLO/PXGs) are a family of multifunctional proteins that are ubiquitous in land plants and also found in some fungi and green algae. CLO/PXGs were initially described as a class of plant lipid-associated proteins with some similarities to the oleosins that stabilize lipid droplets (LDs) in storage tissues such as seeds. However, we now know that CLO/PXGs have more complex structure, distribution and functions than oleosins. Structurally, CLO/PXGs share conserved domains that confer specific biochemical features with diverse localizations and functions.SCOPE: This review surveys the structural properties of CLO/PXGs and their biochemical roles. In addition to their highly conserved structures, CLO/PXGs have peroxygenase activities and are involved in several aspects of oxylipin metabolism in plants. The enzymatic activities and the spatiotemporal expression of CLO/PXGs are described and linked with their wider involvement in plant physiology. Plant CLO/PXGs have many roles in both biotic and abiotic stress responses in plants and in their responses to environmental toxins. Finally, some intriguing developments in the biotechnological uses of CLO/PXGs are addressed.CONCLUSIONS: It is now two decades since caleosin/peroxygenases (CLO/PXGs) were first recognized as a new class of lipid-associated proteins, and only 15 years since their additional enzymatic functions as a novel class of peroxygenases was discovered. There are many interesting research questions that remain to be addressed in future physiological studies of plant CLO/PXGs and also their recently discovered roles in the sequestration and possibly detoxification of a wide variety of lipidic xenobiotics that can challenge plant welfare.</p

Brief for Amicus Curiae Supporting Appellants-Defendants and Urging Reversal, Capitol Mortgage Bankers, Inc. v. Andrew M. Cuomo, Secretary, United States Department of Housing and Urban Development and United States Department of Housing and Urban Development, No. 00-1036

Amici curiae brief filed by St. Ambrose Housing Aid Center, Associated Communities Organized for Reform Now (ACORN), Southeast East Community Organization (SECO), Park Reist Corridor Coalition (PRCC), and the National Training and Information Center (NTIC) on behalf of Defendant-Appellants, Andrew Cuomo, Secretary of the United States Department of Housing and Urban Development (HUD), and HUD. Amici argue that based upon the statutory construction of 12 U.S.C. §1709(r), the Secretary of HUD had the authority to promulgate 24 C.F.R. §202.3 and to subsequently terminate Appellee Capitol Mortgage Bankers’ ability to originate Federal Housing Act (FHA) loans. Alternatively, amici argue that because “flipping” and the associated egregiously high rates of default associated with this practice were unforeseeable by Congress at the time they enacted §533 of the National Housing Act (NHA), the Secretary of HUD was justified based on his reasonable construction of the statute to use his authority under the NHA to terminate Appellee’s ability to originate FHA loans. “Flipping” property is legal, but can be illegal when a mortgagee makes an artificially high loan on a property as a result of the property having been overvalued, and the mortgagor’s qualifications to buy the property having been most likely misstated. High default rates are characteristic of illegal flipping and leads to community destabilization, “homeowner flight”, and under investment by legitimate banks and businesses

Identification of a dioxin-responsive oxylipin signature in roots of date palm:involvement of a 9-hydroperoxide fatty acid reductase, caleosin/peroxygenase PdPXG2

Abstract Dioxins are highly hazardous pollutants that have well characterized impacts on both animal and human health. However, the biological effects of dioxins on plants have yet to be described in detail. Here we describe a dioxin-inducible caleosin/peroxygenase isoform, PdPXG2, that is mainly expressed in the apical zone of date palm roots and specifically reduces 9-hydroperoxide fatty acids. A characteristic spectrum of 18 dioxin-responsive oxylipin (DROXYL) congeners was also detected in date palm roots after exposure to dioxin. Of particular interest, six oxylipins, mostly hydroxy fatty acids, were exclusively formed in response to TCDD. The DROXYL signature was evaluated in planta and validated in vitro using a specific inhibitor of PdPXG2 in a root-protoplast system. Comparative analysis of root suberin showed that levels of certain monomers, especially the mono-epoxides and tri-hydroxides of C16:3 and C18:3, were significantly increased after exposure to TCDD. Specific inhibition of PdPXG2 activity revealed a positive linear relationship between deposition of suberin in roots and their permeability to TCDD. The results highlight the involvement of this peroxygenase in the plant response to dioxin and suggest the use of dioxin-responsive oxylipin signatures as biomarkers for plant exposure to this important class of xenobiotic contaminants

Oil palm in the 2020s and beyond: challenges and solutions

Oil palm, Elaeis guineensis, is by far the most important global oil crop, supplying about 40\\% of all traded vegetable oil. Palm oils are key dietary components consumed daily by over three billion people, mostly in Asia, and also have a wide range of important non-food uses including in cleansing and sanitizing products.info:eu-repo/semantics/publishedVersio

Effects of dioxins on animal spermatogenesis:A state-of-the-art review

The male reproductive system is especially affected by dioxins, a group of persistent environmental pollutants, resulting in irreversible abnormalities including effects on sexual function and fertility in adult males and possibly on the development of male offspring. The reproductive toxicity caused by dioxins is mostly mediated by an aryl hydrocarbon receptor (AhR). In animals, spermatogenesis is a highly sensitive and dynamic process that includes proliferation and maturation of germ cells. Spermatogenesis is subject to multiple endogenous and exogenous regulatory factors, including a wide range of environmental toxicants such as dioxins. This review discusses the toxicological effects of dioxins on spermatogenesis and their relevance to male infertility. After a detailed categorization of the environmental contaminants affecting the spermatogenesis, the exposure pathways and bioavailability of dioxins in animals was briefly reviewed. The effects of dioxins on spermatogenesis are then outlined in detail. The endocrine-disrupting effects of dioxins in animals and humans are discussed with a particular focus on their effects on the expression of spermatogenesis-related genes. Finally, the impacts of dioxins on the ratio of X and Y chromosomes, the status of serum sex hormones, the quality and fertility of sperm, and the transgenerational effects of dioxins on male reproduction are reviewed