Prediction and Identification of Potential Immunodominant Epitopes in Glycoproteins B, C, E, G, and I of Herpes Simplex Virus Type 2

Twenty B candidate epitopes of glycoproteins B (gB2), C (gC2), E (gE2), G (gG2), and I (gI2) of herpes simplex virus type 2 (HSV-2) were predicted using DNAstar, Biosun, and Antheprot methods combined with the polynomial method. Subsequently, the biological functions of the peptides were tested via experiments in vitro. Among the 20 epitope peptides, 17 could react with the antisera to the corresponding parent proteins in the EIA tests. In particular, five peptides, namely, gB2466–473 (EQDRKPRN), gC2216–223 (GRTDRPSA), gE2483–491 (DPPERPDSP), gG2572–579 (EPPDDDDS), and gI2286-295 (CRRRYRRPRG) had strong reaction with the antisera. All conjugates of the five peptides with the carrier protein BSA could stimulate mice into producing antibodies. The antisera to these peptides reacted strongly with the corresponding parent glycoproteins during the Western Blot tests, and the peptides reacted strongly with the antibodies against the parent glycoproteins during the EIA tests. The antisera against the five peptides could neutralize HSV-2 infection in vitro, which has not been reported until now. These results suggest that the immunodominant epitopes screened using software algorithms may be used for virus diagnosis and vaccine design against HSV-2

Responses of Phaseolus calcaltus to lime and biochar application in an acid soil

Introduction Rice bean (Phaseolus calcaltus), as an annual summer legume, is always subjected to acid soils in tropical to subtropical regions, limiting its growth and nodulation. However, little is known about its responses to lime and biochar addition, the two in improving soil fertility in acid soils. Materials and Methods In the current study, a pot experiment was conducted using rice bean on a sandy yellow soil (Orthic Acrisol) with a pH of 5.5. The experiment included three lime rates (0, 0.75 and 1.5 g kg−1) and three biochar rates (0, 5 and 10 g kg−1). The biochar was produced from aboveground parts of Solanum tuberosum using a home-made device with temperature of pyrolysis about 500 °C. Results and Discussion The results indicated that both lime and biochar could reduce soil exchange Al concentration, increase soil pH and the contents of soil microbial biomass carbon and microbial biomass nitrogen, and enhance urease and dehydrogenase activities, benefiting P. calcaltus growth and nodulation in acid soils. Lime application did decrease the concentrations of soil available phosphorus (AP) and alkali dispelled nitrogen (AN), whereas biochar application increased the concentrations of soil AP, AN and available potassium (AK). However, sole biochar application could not achieve as much yield increase as lime application did. High lime rate (1.5 g lime kg−1) incorporated with low biochar rate (5 g biochar kg−1) could obtain higher shoot biomass, nutrient uptake, and nodule number when compared with high lime rate and high biochar rate. Conclusion Lime incorporated with biochar application could achieve optimum improvement for P. calcaltus growing in acid soils when compared with sole lime or biochar addition

Ivermectin induces apoptosis of esophageal squamous cell carcinoma via mitochondrial pathway

Background: Esophageal squamous cell carcinoma (ESCC) is the most predominant primary malignant tumor among worldwide, especially in China. To date, the successful treatment remains a mainly clinical challenge, it is imperative to develop successful therapeutic agents. Methods: The anti-proliferative effect of ivermectin on ESCC is investigated in cell model and in nude mice model. Cell apoptosis was assessed using flow cytometry, TUNEL assay and western blotting. Mitochondrial dysfunction was determined by reactive oxygen species accumulation, mitochondrial membrane potential and ATP levels. Results: Our results determined that ivermectin significantly inhibited the proliferation of ESCC cells in vitro and in vivo. Furthermore, we found that ivermectin markedly mediated mitochondrial dysfunction and induced apoptosis of ESCC cells, which indicated the anti-proliferative effect of ivermectin on ESCC cells was implicated in mitochondrial apoptotic pathway. Mechanistically, ivermectin significantly triggered ROS accumulation and inhibited the activation of NF-κB signaling pathway and increased the ratio of Bax/Bcl-2. Conclusions: These finding indicated that ivermectin has significant anti-tumour potential for ESSC and may be a potential therapeutic candidate against ESCC

Grouting Fractured Coal Permeability Evolution Based on Industrial CT Scanning

Gas extraction from coal seams in China faces various middle-term and long-term problems, such as the poor sealing quality and low extraction rate. The mean gas extraction concentration is only 30%. Studying the flowing laws of the grout and fracture plugging mechanism is of important significance to improve the sealing quality and increase the gas extraction efficiency. For this reason, a new coal-based grouting material was prepared in this study, and its parameters such as viscosity were tested. Moreover, a grouting theoretical model with considerations of the flowing of the grout and coal fracture plugging by migration and deposition of slurry particles was constructed. The crack distribution before and after the grouting of fractured coal samples was scanned with an industrial CT, and the fracture distribution of coal samples was reconstructed using an independently compiled MATLAB program. Meanwhile, the variations of the coal permeability before and after the grouting were tested. On this basis, this study built a numerical calculation model of the grouting in fractured coal samples to simulate the leakage stoppage and permeability reduction mechanism of the coal-based sealing material grouting. Results demonstrate that (1) according to the experimental test results, the new coal-based grouting materials achieve a good fracture plugging effect, and the fractures in coal samples after the grouting are filled densely by the grouting particles. (2) According to the simulation results, the permeability of grouting coal samples declines quickly and then tends to be stable. The overall permeability of coal samples and the fracture permeability are decreased by 93.5% and more than 99.9% in average, respectively. (3) Influences of the grouting pressure on the permeability variation of coal samples were investigated through a numerical simulation. It was found that a reasonable grouting pressure for coal samples is about 0.3 MPa. (4) The numerical simulation reproduces the whole process of the grout flowing and the fracture filling. The variation laws of the diffusion and the permeability of the grout which are calculated through a numerical simulation agree well with the experimental results, which verifies the reasonability of the model. Research conclusions can provide important significance in theory and practice to disclose the leakage stoppage and the permeability reduction mechanism of the borehole grouting during the gas extraction and strengthen the sealing effects of extraction boreholes

Effect of cyclic temperature impact on coal seam permeability

The gas permeability of the coal samples, subjected to cyclic temperature impact under different conditions, was considered in the article. Meanwhile, the change of the coal sample permeability and fractures were analyzed through scanning electron microscopy. The obtained results show that the cyclic temperature impact has largely positive effect on the increase of the coal sample permeability and that new fractures and permeability growth increase with the higher temperature difference

Multi-Hand Gesture Recognition Using Automotive FMCW Radar Sensor

With the development of human–computer interaction(s) (HCI), hand gestures are playing increasingly important roles in our daily lives. With hand gesture recognition (HGR), users can play virtual games together, control the smart equipment, etc. As a result, this paper presents a multi-hand gesture recognition system using automotive frequency modulated continuous wave (FMCW) radar. Specifically, we first constructed the range-Doppler map (RDM) and range-angle map (RAM), and then suppressed the spectral leakage, and dynamic and static interferences. Since the received echo signals with multi-hand gestures are mixed together, we propose a spatiotemporal path selection algorithm to separate the mixed multi-hand gestures. A dual 3D convolutional neural network-based feature fusion network is proposed for feature extraction and classification. We developed the FMCW radar-based platform to evaluate the performance of the proposed multi-hand gesture recognition method; the experimental results show that the proposed method can achieve an average recognition accuracy of 93.12% when eight gestures with two hands are performed simultaneously

Phenotypic Plasticity and Local Adaptation of Leaf Cuticular Waxes Favor Perennial Alpine Herbs under Climate Change

Six perennial herbs (Plantago asiatica, Polygonum viviparum, Anaphalis lactea, Kobresia humilis, Leontopodium nanum and Potentilla chinensis) widely distributed in alpine meadows were reciprocally transplanted at two sites in eastern edge of Qinghai-Tibetan Plateau, Hongyuan (3434 m, 2.97 °C, 911 mm) and Qilian (3701 m, 2.52 °C, 472 mm), aiming to evaluate the responses of alpine plants to changing environments. When plants were transplanted from Hongyuan to Qilian, most plant species showed a decrease of total wax coverage in first year and reverse trend was observed for some plant species in second year. However, when plants were transplanted from Qilian to Hongyuan, the response of total wax coverage differed greatly between plant species. When compared with those in first year, plasticity index of average chain length of alkane decreased whereas carbon preference index of alkane increased at both Hongyuan and Qilian in second year. The total wax coverage differed between local and transplanted plants, suggesting both environmental and genetic factors controlled the wax depositions. Structural equation modeling indicated that co-variations existed between leaf cuticular waxes and leaf functional traits. These results suggest that alpine herbs adjust both wax depositions and chain length distributions to adapt to changing environment, showing climate adaptations

Effects of fertilizations on soil bacteria and fungi communities in a degraded arid steppe revealed by high through-put sequencing

Background Fertilization as one of the measures in restoring degraded soil qualities has been introduced on arid steppes in recent decades. However, the fertilization use efficiency on arid steppes varies greatly between steppe types and years, enhancing uncertainties and risks in introducing fertilizations on such natural system to restore degraded steppes. Methods The experiment was a completely randomized design with five fertilization treatments, 0 (Control), 60 kg P ha−1 (P), 100 kg N ha−1 (N), 100 kg N ha−1 plus 60 kg P ha−1 (NP), and 4,000 kg sheep manure ha−1 (M, equaling 16.4 kg P ha−1 and 81.2 kg N ha−1). Soils were sampled from a degraded arid steppe which was consecutively applied with organic and inorganic fertilizers for three years. We analyzed the diversity and abundance of soil bacteria and fungi using high-throughput sequencing technique, measured the aboveground biomass, the soil chemical properties (organic carbon, available and total phosphorus, available and total nitrogen, and pH), and the microbial biomass nitrogen and microbial biomass carbon. Results In total 3,927 OTU (operational taxonomic units) for bacteria and 453 OTU for fungi were identified from the tested soils. The Ace and Chao of bacteria were all larger than 2,400, which were almost 10 times of those of fungi. Fertilizations had no significant influence on the richness and diversity of the bacteria and fungi. However, the abundance of individual bacterial or fungi phylum or species was sensitive to fertilizations. Fertilization, particularly the phosphorus fertilizer, influenced more on the abundance of the AMF species and colonization. Among the soil properties, soil pH was one of the most important soil properties influencing the abundance of soil bacteria and fungi. Discussion Positive relationships between the abundance of bacteria and fungi and the soil chemical properties suggested that soil bacteria and fungi communities in degraded steppes could be altered by improving the soil chemical properties through fertilizations. However, it is still not clear whether the alteration of the soil microbe community is detrimental or beneficial to the degraded arid steppes