Designing a Low-Cost Mechatronic Device for Semi-Automatic Saffron Harvesting

This paper addresses the design of a novel mechatronic device for saffron harvesting. The main proposed challenge consists of proposing a new paradigm for semi-automatic harvesting of saffron flowers. The proposed novel solution is designed for being easily portable with user-friendly and cost-oriented features and with a fully electric battery-powered actuation. A preliminary concept design has been proposed as based on a specific novel cam mechanism in combination with an elastic spring for fulfilling the detachment of the flowers from their stems. Numerical calculations and simulations have been carried out to complete the full design of a proof-of-concept prototype. Preliminary experimental tests have been carried out to demonstrate the engineering feasibility and effectiveness of the proposed design solutions, whose concept has been submitted for patenting

Cooperation between Constitutive and Inducible Chemokines Enables T Cell Engraftment and Immune Attack in Solid Tumors

© 2019 Elsevier Inc. We investigated the role of chemokines in regulating T cell accumulation in solid tumors. CCL5 and CXCL9 overexpression was associated with CD8+ T cell infiltration in solid tumors. T cell infiltration required tumor cell-derived CCL5 and was amplified by IFN-γ-inducible, myeloid cell-secreted CXCL9. CCL5 and CXCL9 coexpression revealed immunoreactive tumors with prolonged survival and response to checkpoint blockade. Loss of CCL5 expression in human tumors was associated with epigenetic silencing through DNA methylation. Reduction of CCL5 expression caused tumor-infiltrating lymphocyte (TIL) desertification, whereas forced CCL5 expression prevented Cxcl9 expression and TILs loss, and attenuated tumor growth in mice through IFN-γ. The cooperation between tumor-derived CCL5 and IFN-γ-inducible CXCR3 ligands secreted by myeloid cells is key for orchestrating T cell infiltration in immunoreactive and immunoresponsive tumors. Dangaj et al. show that tumor cell-expressed CCL5 and macrophage- and DC-expressed CXCL9 are important for the infiltration of T cells into tumors, a process that also requires recognition of tumor antigens by T cells. CCL5 is often epigenetically silenced in tumor cells but can be reactivated by Decitabine