3 research outputs found
Activity of antioxidative defense enzymes in the blood of patients with liver echinococcosis
Background/Aim. Chronic echinococcocal disease is the parasite human disease caused by the penetration of larval (asexual) stages of the canine tapeworm (Echinococcus granulosus) in the liver of humans. After the penetration of the parasite, the host organism react by activating complement- depending immune response. The aim of this study was to elucidate the influence of larval form of Echinococcus granulosus in the liver on the activity of antioxidative defense enzymes in the blood of patients before and after the surgical intervention. Methods. We investigated the activity of antioxidative defense enzymes: copper/zinc containing superoxide dismutase (CuZn SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR) and glutathione-S-transferase (GST) in the blood of patients before and after the surgical intervention in respect to the controls, clinically healthy persons. Results. Our results showed that the activity of the GSH-Px was significantly decreased in the plasma of the patients with echinocococal disease before the surgery in respect to the controls. The activity of GST was significantly higher in the blood of the patients after the surgery in comparison to the controls. Conclusion. Chronic liver echinoccocal disease caused significant changes of some antioxidative defense enzymes, first of all Se-dependent enzyme GSH-Px, which could be a suitabile biomarker in the biochemical evaluation of the disease. This work represents a first comprehensive study of the activity of antioxidative defense enzymes in cronic liver echinococcocosis in the patients before and after the surgical intervention in respect to the clinically healthy persons
Activity of antioxidative defense enzymes in the blood of patients with liver echinococcosis
Background/Aim. Chronic echinococcocal disease is the parasite human disease caused by the penetration of larval (asexual) stages of the canine tapeworm (Echinococcus granulosus) in the liver of humans. After the penetration of the parasite, the host organism react by activating complement- depending immune response. The aim of this study was to elucidate the influence of larval form of Echinococcus granulosus in the liver on the activity of antioxidative defense enzymes in the blood of patients before and after the surgical intervention. Methods. We investigated the activity of antioxidative defense enzymes: copper/zinc containing superoxide dismutase (CuZn SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR) and glutathione-S-transferase (GST) in the blood of patients before and after the surgical intervention in respect to the controls, clinically healthy persons. Results. Our results showed that the activity of the GSH-Px was significantly decreased in the plasma of the patients with echinocococal disease before the surgery in respect to the controls. The activity of GST was significantly higher in the blood of the patients after the surgery in comparison to the controls. Conclusion. Chronic liver echinoccocal disease caused significant changes of some antioxidative defense enzymes, first of all Se-dependent enzyme GSH-Px, which could be a suitabile biomarker in the biochemical evaluation of the disease. This work represents a first comprehensive study of the activity of antioxidative defense enzymes in cronic liver echinococcocosis in the patients before and after the surgical intervention in respect to the clinically healthy persons.Uvod/Cilj. Ehinokokusna bolest je parazitno oboljenje koje nastaje prodorom larvene forme pseÄe pantljiÄare (Echinococcus granulosus) u jetru Äoveka, pri Äemu dolazi do reakcije domaÄina koji se brani od infektivnog agensa aktivacijom sistema komplementa i komplement-zavisnim imunim odgovorom. Cilj ovog rada bio je da se utvrdi da li razvoj larvenog oblika pseÄe pantljiÄare (Echinococcus granulosus) u jetri ima uticaja na aktivnost enzima sistema zaÅ”tite od oksidacionih oÅ”teÄenja u krvi obolelih pre i posle operativnog zahvata. Metode. Ispitivane su promene aktivnosti enzima sistema zaÅ”tite od oksidacionih oÅ”teÄenja: bakar/cink sadržavajuÄe superoksid-dismutaze (CuZn SOD), katalaze (CAT), glutation-peroksidaze (GSH-Px), glutation-reduktaze (GR) i glutation-S-transferaze (GST) u krvi obolelih od ehinokokusne bolesti jetre pre i posle operacije u odnosu na kontrolne, kliniÄki zdrave osobe. Rezultati. Ustanovljen je statistiÄki znaÄajan pad aktivnosti enzima GSH-Px u plazmi bolesnika obolelih od ehinokokusne bolesti jetre pre operacije u odnosu na kontrolu. Aktivnost enzima GST bila je statistiÄki znaÄajno poveÄana u krvi obolelih od hroniÄne ehinokokusne bolesti jetre posle operacije u odnosu na kontrolne vrednosti. ZakljuÄak. HroniÄna ehinokokusna bolest jetre izaziva promene aktivnosti nekih enzima sistema zaÅ”tite od oksidacionih oÅ”teÄenja, pre svega Se-zavisnog enzima GSH-Px, Å”to može predstavljati dobar biomarker u daljoj biohemijskoj evaluaciji ove bolesti. Prema dostupnim podacima ovaj rad predstavlja prvu studiju aktivnosti enzima sistema zaÅ”tite od oksidacionih oÅ”teÄenja kod obolelih od hroniÄne ehinokokusne bolesti jetre pre i posle operacije u odnosu na kontrolne, kliniÄki zdrave osobe.nul
Activity of antioxidative defense enzymes in the blood of patients with liver echinococcosis
Background/Aim. Chronic echinococcocal disease is the parasite human disease caused by the penetration of larval (asexual) stages of the canine tapeworm (Echinococcus granulosus) in the liver of humans. After the penetration of the parasite, the host organism react by activating complement- depending immune response. The aim of this study was to elucidate the influence of larval form of Echinococcus granulosus in the liver on the activity of antioxidative defense enzymes in the blood of patients before and after the surgical intervention. Methods. We investigated the activity of antioxidative defense enzymes: copper/zinc containing superoxide dismutase (CuZn SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR) and glutathione-S-transferase (GST) in the blood of patients before and after the surgical intervention in respect to the controls, clinically healthy persons. Results. Our results showed that the activity of the GSH-Px was significantly decreased in the plasma of the patients with echinocococal disease before the surgery in respect to the controls. The activity of GST was significantly higher in the blood of the patients after the surgery in comparison to the controls. Conclusion. Chronic liver echinoccocal disease caused significant changes of some antioxidative defense enzymes, first of all Se-dependent enzyme GSH-Px, which could be a suitabile biomarker in the biochemical evaluation of the disease. This work represents a first comprehensive study of the activity of antioxidative defense enzymes in cronic liver echinococcocosis in the patients before and after the surgical intervention in respect to the clinically healthy persons.Uvod/Cilj. Ehinokokusna bolest je parazitno oboljenje koje nastaje prodorom larvene forme pseÄe pantljiÄare (Echinococcus granulosus) u jetru Äoveka, pri Äemu dolazi do reakcije domaÄina koji se brani od infektivnog agensa aktivacijom sistema komplementa i komplement-zavisnim imunim odgovorom. Cilj ovog rada bio je da se utvrdi da li razvoj larvenog oblika pseÄe pantljiÄare (Echinococcus granulosus) u jetri ima uticaja na aktivnost enzima sistema zaÅ”tite od oksidacionih oÅ”teÄenja u krvi obolelih pre i posle operativnog zahvata. Metode. Ispitivane su promene aktivnosti enzima sistema zaÅ”tite od oksidacionih oÅ”teÄenja: bakar/cink sadržavajuÄe superoksid-dismutaze (CuZn SOD), katalaze (CAT), glutation-peroksidaze (GSH-Px), glutation-reduktaze (GR) i glutation-S-transferaze (GST) u krvi obolelih od ehinokokusne bolesti jetre pre i posle operacije u odnosu na kontrolne, kliniÄki zdrave osobe. Rezultati. Ustanovljen je statistiÄki znaÄajan pad aktivnosti enzima GSH-Px u plazmi bolesnika obolelih od ehinokokusne bolesti jetre pre operacije u odnosu na kontrolu. Aktivnost enzima GST bila je statistiÄki znaÄajno poveÄana u krvi obolelih od hroniÄne ehinokokusne bolesti jetre posle operacije u odnosu na kontrolne vrednosti. ZakljuÄak. HroniÄna ehinokokusna bolest jetre izaziva promene aktivnosti nekih enzima sistema zaÅ”tite od oksidacionih oÅ”teÄenja, pre svega Se-zavisnog enzima GSH-Px, Å”to može predstavljati dobar biomarker u daljoj biohemijskoj evaluaciji ove bolesti. Prema dostupnim podacima ovaj rad predstavlja prvu studiju aktivnosti enzima sistema zaÅ”tite od oksidacionih oÅ”teÄenja kod obolelih od hroniÄne ehinokokusne bolesti jetre pre i posle operacije u odnosu na kontrolne, kliniÄki zdrave osobe.nul