Does daily consumed herbal tea have an inhibitory effect on dental plaque formation?

In this study, developmental conditions of some bacteria responsible for the formation of dental plaque on teeth were identified, levels of dental plaque formation were determined, and the antimicrobial effect of some herbal extracts on the biofilm producing bacteria was investigated. For this purpose, 9 dental bacterial strains and Streptococcus mutans ATCC 25175 selected as standard were allowed to create biofilm on dental composite material. In later stages, the bacterial numbers on the restorative material were determined. Then, inhibitory effects of green tea, black tea, linden, sage, ginger and cinnamon extracts, which are often consumed in daily life, on bacteria responsible for dental plaque formation was examined. To the results, almost all of the isolates and S.mutans found to form biofilm in the range of 3.62 to 5.05 log CFU/mm2 on the dental composite resin surface while the highest attachment was determined as 4.71 log CFU/mm2 for S. mutans. Also, all of the herbal extracts showed antimicrobial activity at different levels. Besides, the dental strains were identified as different species of Enterobacter, Halanaerobacter, Klebsiella, Staphylococcus, Streptococcus, Thermoproteus genera. Finally, it was found that cinnamon, ginger and green tea presented the highest inhibitory effect among plant extracts used in the study.Ankara Üniversitesi Biyoteknoloji Enstitüsü Laboratuvar

Farklı cila sistemlerinin nano-dolduruculu kompozitlerin yüzey pürüzlülüğü üzerine etkisi

Bu çalışmanın amacı farklı cila sistemleri kullanarak iki farklı doldurucu türüne sahip kompozit rezin materyalin yüzey pürüzlülük özelliklerini incelemektir. Bu amaçla iki farklı nanokompozit Grandio, VOCO ve Filtek SupremeUltra Enamel, 3M/ESPE ve üç farklı cila sistemi kullanılmıştır. Her bir kompozit materyalden 40’ar adet örnek hazırlanmıştır N=80 . Tüm örnekler pleksicam kalıplarda hazırlanmış ve polyester bant kullanılarak polimerize edilmiştir. Örnekler farklı cila işlemleri uygulanmak üzere 4 alt gruba ayrılmıştır n=10 . Kontrol grubu Grup K haricindeki diğer gruplara, önce karpit frez ile bitirme işlemi uygulanmış, sonrasında şu cila sistemleri uygulanmıştır: Soft-Lex XT Grup SL , PoGo Grup PG , Astropol Grup AS .Örnekler; yüzey pürüzlülüğündeki değişimlerin değerlendirilebilmesi için optik profilometre kullanılarak incelenmiştir. Örneklerin ortalama yüzey pürüzlülüğü Ra verilerinin istatistiksel olarak değerlendirilmesinde çift yönlü varyans analizi, ortalamaların çoklu karşılaştırılmasında ise Tukey HSD testi kullanılmıştır p=0,05 . İki kompozit rezin grubu değerlendirildiğinde en düşük Ra değeri Filtek Supreme XT Enamel grubununda izlenmektedir. Grandio grubunun Ra değeri daha yüksektir ve bu farklılık istatistiksel olarak anlamlıdır

The Effect of Gravity on Marginal Integrity of Different Flowable Bulk-Fill Resin Composites

Background and Objectives: The aim of this quantitative research was to investigate the effect of gravitational forces on the marginal integrity of different bulk-fill composites by micro-CT imaging. Materials and Methods: Fifty caries-free human third molars extracted for prophylactic purposes were used in this study. Each tooth was prepared with two proximal box cavities, with dimensions of 3 mm × 3 mm × 5 mm. Five distinct groups, each comprising 20 cavities, thus totaling 100 cavities for this study: (1, Group CON): Clearfil Majesty Flow + Clearfil Majesty Esthetic (as the control); (2, Group FBR): Filtek Bulk-fill Flowable Restorative + Clearfil Majesty Esthetic; (3, Group XTB): Voco Extrabase + Clearfil Majesty Esthetic; (4, Group SDR): SDR + Clearfil Majesty Esthetic; and (5, Group SNC): Sonicfill. When restoring the mesial cavities, the occlusal surfaces of the teeth in the mold were positioned upwards, counteracting the force of gravity. In contrast, for the restoration of the distal cavities, the occlusal surfaces were aligned downwards, to be parallel with the gravitational pull. After restorative procedures, each tooth was treated with 5000 thermal cycles. A solution of ammoniacal silver nitrate (AgNO3) was employed as a tracing agent. The micro-CT scans were conducted and the total volume of silver nitrate and the total volume of restorations within the relevant region of interest were calculated in “mm3” with software. Two-way ANOVA and Tukey tests were performed at a significance level of p = 0.05 with Graphpad Prism v 8.2.1 software. Results: Both gravity effect and interaction showed no statistical differences (p > 0.05). Statistically significant differences were observed in the restorative materials (p Conclusions: Gravitational forces do not emerge as a major factor affecting the marginal integrity of flowable bulk-fill composites in class II restorations. The chemical composition of the composites plays a more crucial role, with the XTB composite showing higher microleakage ratios compared to the others

Comparison of Stain Effects of Nicotine and Beverages on Different Cad-Cam Materials

Purpose: This study aimed to compare the effect of smoking cigarettes and drinking beverages and also polishing procedures on the discoloration of CAD/CAM materials. Materials and Methods: Resin nanoceramic (Lava Ultimate-RN), feldspar ceramic (CEREC Blocs Ceramics-FC) and lithium disilicate glass-ceramic (IPS e.max® CAD-LDC) specimens (5X5 X2 mm) were used for this study. Initially, baseline surface roughness (Ra) and color values (L*, a*, b*) of the specimens were measured with profilometer and chroma meter. Then the specimens were exposure nicotine, coffee and red wine. The first discoloration values of each specimen were measured. Then, all of the specimens were subjected to re-polishing procedures. Than, the surface roughness and color values of each specimen were measured. The specimens were subjected to the same staining process again. After exposure of the specimens to the staining agents, the second staining value was measured. Two-way analysis of variance (ANOVA) was used for normally distributed groups, and the Kruskal-Wallis test was used for non-normally distributed groups. Results: Statistically significant ΔE* values were observed for RN (8.26±1.55) and FC (7.69±1.52) for nicotine in the first staining group. The maximum total color changes were observed in the nicotine group for all of the material specimens. Nicotine caused the highest color changes on the test materials than coffee and wine. Surface roughness did not show a statistically significant difference based on the staining agents for any of the materials within a group (p lt;0.05). Conclusions: It was observed that nicotine staining is far stronger than beverage staining

In-vitro analysis of maxillary first molars morphology using three dimensional Micro-CT imaging: considerations for restorative dentistry

Purpose The aim of this study was to determine the differences between the positional relationship of the crown contour and the pulp chamber of left and right maxillary first molars, as well as their morphological characteristics by using micro-CT system with reconstruction from a volumetric rendering software. Materials and methods In total, 21 extracted maxillary first molars, including 11 left and 10 right teeth, were used. The positional relationship between the crown contour, pulp chamber and morphology of the teeth were investigated three-dimensionally by means of micro-CT imaging. Results Closest distance of mesio-buccal pulp horn to enamel surface in mm was calculated as 2.5±0.20 mm for right and 2.29±0.17 mm for left teeth. This difference was statistically significant (p=0.017). The means of closest distance of disto-buccal pulp horn to enamel surface were also significantly different between left and right teeth (p=0.001). The mean pulp volumes of right side and left side teeth were, respectively, 32.94±3.19 mm3 and 33.71±2.82 mm3 but this difference was not statistically significant. Conclusion These results suggest that right and left maxillary first molars should be treated differently during preparation of cavities. Further studies must be done with larger samples as well as for other molar teeth in different populations to reveal the morphology of the molar for further considerations in restorative dentistry

Marginal adaptation of bulk-fill resin composites with different viscosities in class II restorations: a micro-CT evaluation

Abstract Background The purpose of this study is to evaluate the marginal adaptation of bulk-fill resin composites with different viscosities (paste-like and flowable) in Class II restorations using micro-CT imaging. Methods Forty extracted human molars were used. Mesial and distal Class II box cavities (approximately 3 mm x 3 mm x 4 mm) were prepared for each tooth, with cavity floors located 1 mm below the enamel-cementum junction. Following adhesive application, teeth were restored using eight different groups: Group XB: X-tra Base Bulk-fill Flowable (VOCO), Group XF: X-tra Fill Bulk-fill (VOCO), Group FB: Filtek Bulk-fill Posterior (3 M ESPE), Group FF: Filtek Bulk-fill Flowable (3 M ESPE), Group BB: Beautifil-Bulk (SHOFU), Group BF: Beautifil-Bulk Flowable (SHOFU), and Group CO: “as a control group”, Clearfil Majesty Posterior (KURARAY) and Group CF: “as a control group”, Clearfil Majesty Flow + Clearfil Majesty Posterior (KURARAY). The restored teeth underwent an aging protocol involving 1000 cycles in a water bath fluctuating between 5 ± 1.0 °C and 55 ± 1.0 °C. Post-aging, teeth were immersed in 50% silver nitrate solution for 24 h and then in a film developer solution for 8 h. Microleakage analysis was performed using micro-CT, evaluated with 3D Slicer software. A two-way ANOVA was employed for statistical analysis. Results Two-way ANOVA results indicated significant effects of both viscosity (p < 0.0001) and composite type (p < 0.0001) on marginal adaptation. Viscosity analysis (comparing flowable and paste-like) revealed no significant differences in the FB-FF, XB-XF and BB-BF groups but significant differences in the and CO-CF group, with flowable type exhibiting less microleakage than paste-like type. Conclusions The study suggests that while the viscosity of bulk-fill composites did not significantly affect marginal adaptation, the brand of bulk-fill composite did influence it