Detection of Adenovirus, Respiratory Syncytial Virus and Parainfluenza Virus Infections in Our Region in the 1995-96 Winter Season by Cell Culture Method

The frequency and distribution of respiratory syncytial virus (RSV), adenovirus and parainfluenza virus, the important causes of respiratory tract infections in our region were investigated in this study. For this reason, nasopharyngeal swab specimens were collected from 187 patients (131 children and 56 adults) who suffered from influenza-like illness in the winter of 1995-96. Specimens were inoculated on Hep-2 cells for RSV and adenovirus, and on MDBK cells for parainfluenza virus isolation. The tubes were examined for the cytopathic effect every other day for 15 days and hemagglutination test was performed for parainfluenza virus identification. Cytopathic effect and/or hemagglutination test positive specimens were stained using fluorescein-conjugated monoclonal antibodies and examined under fluorescent microscope. RSV was isolated from 6 children, adenovirus from 7 patients, five of them were adults, and human parainfluenza viruses type 1 and 2 each from one child patient. As a result: A respiratory tract pathogen was isolated from 15 of all 187 (%8) nasopharyngeal swab specimens

1995-1996 kış mevsiminde, hücre kültürü yöntemi ile bölgemizde saptanan adenovirüs, solunum sinsityal virüsü ve parainfluenza virüsü infeksiyonları

The frequency and distribution of respiratory syncytial virus (RSV), adenovirus and Para influenza virus, the important causes of respiratory tract infections in our region were investigated in this study. For this reason, nasopharyngeal swab specimens were collected from 187 patients (131 children and 56 adults) who suffered from influenza-like illness in the winter of 1995-96. Specimens were inoculated on Hep-2 cells for RSV and adenovirus, and on MDBK cells for Para influenza virus isolation. the cubes were examined for the cytopathic effect every other day for 15 days and hemagglutination test was performed for parainfluenza virus identification.-Cytopathic effect and/or hemagglutination test positive specimens were stained using fluorescein-conjugated monoclonal antibodies and examined under fluorescent microscope. RSV was isolated from 6 children, adenovirus from 7 patients; five of them were adults, and human Para influenza viruses' type l and 2 each from one child patient. As a result: A respiratory tract pathogen was isolated from 15 of all 187 (%8) nasopharyngeal swab specimens.Bu çalışmada, solunum sistemi hastalıklarının önemli etkenlerinden olan solunum sinsityal virüsü, adenovirüs ve parainfluenza virüslerinin bölgemizdeki dağılımı ve sıklığı araştırılmıştır. Bu amaçla 1995-96 kış mevsiminde influenza benzeri hastalık tablosu gösteren, 131'i çocuk ve 56'sı erişkin olmak üzere toplam 187 hastanın nazofaringeal sürüntü örnekleri alınmıştır. Alınan örnekler, solunum sinsityal virüsü (SSV) ve adenovirüs izolasyonu için Hep-2 hücrelerine, parainfluenza virüsü izolasyonu için ise Madine-Darby Bovine Kidney (MDBK) hücreleri üzerine inoküle edilmiştir. Tüpler 15 gün boyunca günaşırı, sitopatik etki yönünden araştırılmış, ayrıca parainfluenza virüslerinin tanısı için yine günaşırı olmak üzere hemaglütinasyon testi yapılmıştır. Sitopatik etki gözlenen ve/veya hemaglütinasyon olumlu olan örnekler, floresein ile işaretli monoklonal antikorlar kullanılarak boyanmış ve floresein mikroskopta incelenmiştir. Yapılan çalışma sonucunda, 6 çocuk hastadan SSV, beşi erişkin olmak üzere 7 hastadan adenovirüs, 2 çocuk hastadan ise parainfluenza virüsü tip 1 ve tip 2 izole edilmiştir. Sonuç olarak toplam 187 örneğin 15'inde (%8) viral bir solunum yolu patojeni saptanmıştır

Splenektomiden 46 yıl sonra sepsis gelişen bir olgu nedeniyle splenektomi sonrası sepsis: Korunma ve öneriler

The mortality rate of postsplenectomy sepsis is 50-70 %, despite appropriate antimicrobial therapy and intensive medical support. For this reason the early diagnosis, treatment, prevention and control of infections in splenectomized patients is an important issue. In this Article, it is aimed to review the approach to the splenectomized patients in an occasion of a case lost by sepsis and pulmonary embolism 46 years after the splenectomy.Splenektomi sonrası sepsislerde uygun antibiyotik ve destek tedavisine rağmen mortalite oranı % 50 ile % 70 arasında değişmektedir. Bu nedenle splenektomili olgularda gelişebilecek infeksiyonların erken tanısı, tedavisi, korunma ve kontrol önlemlerinin uygulanması önem kazanmaktadır. Bu yazıda splenektomiden 46 yıl sonra sepsis ve akciğer embolisi ile kaybedilen bir olgu nedeniyle splenektomili olgulara yaklaşımın gözden geçirilmesi amaçlanmıştır

Attitudes towards HIV/AIDS and other sexually transmitted diseases in secondary school students in Izmir, Turkey: changes in time

WOS: 000244345700004PubMed ID: 17326878The aim of this study was to evaluate the changes in the sexual attitudes of young people in a period of 7 years (1997-2004). The participants in the first and the second surveys were 633 and 654 grade 10 students from six high schools located in the central district of Izmir, Turkey, chosen randomly according to the stratified sampling method. The rate of having had sexual experience was 11.3% and 22.8% in the first and second surveys, respectively (chi(2) P < 0.001)

Programmatic mapping and size estimation of female sex workers, transgender sex workers and men who have sex with men in Istanbul and Ankara, Turkey

Objectives Despite a growing HIV threat, there is no definition and characterisation of key populations (KPs), who could be the major drivers of the epidemic in Turkey. We used programmatic mapping to identify locations where KPs congregate, estimate their numbers and understand their operational dynamics to develop appropriate HIV programme implementation strategies. Methods Female and transgender sex workers (FSWs and TGSWs), and men who have sex with men (MSM) were studied in Istanbul and Ankara. Within each district, hot spots were identified by interviewing key informants and a crude spot list in each district was developed. The spot validation process was led by KP members who facilitated spot access and interviews of KPs associated with that spot. Final estimates were derived by aggregating the estimated number of KPs at all spots, which was adjusted for the proportion of KPs who visit multiple spots, and for the proportion of KPs who do not visit spots. Results FSWs were the largest KP identified in Istanbul with an estimate of 30 447 (5.8/1000 women), followed by 15 780 TGSWs (2.9/1000 men) and 11 656 MSM (2.1/1000). The corresponding numbers in Ankara were 9945 FSWs (5.2/1000 women), 1770 TGSWs (1/1000 men) and 5018 MSM (2.5/1000 men). Each KP had unique typologies based on the way they find and interact with sex partners. MSM were mostly hidden and a higher proportion operated through internet and phone-based applications. Night time was the peak time with Friday, Saturday and Sunday being the peak days of activity in both Istanbul and Ankara. Conclusions This study has highlighted the presence of a substantial number of FSWs, TGSW and MSM in Istanbul and Ankara. The information obtained from this study can be used to set priorities for resource allocation and provide HIV prevention services where coverage could be the highest.Gilead SciencesThis project was funded by a non-conditional grant from Gilead Sciences