Incidencia de la Rugosidad de un Rodillo y otros Factores en el Cálculo de Incertidumbre en la Calibración de un Frenómetro

El calibrado de un frenómetro consiste en comparar la fuerza de frenado con un patrón de fuerza conocido y evaluar su incertidumbre como medida de la calidad de la calibración. En una auditoria del sistema de gestión de la calidad del laboratorio Calibra Uno, bajo la dirección del Organismo Argentino de Acreditación (OAA), se determinó que la rugosidad de los rodillos del frenómetro no había sido tenida en cuenta entre los factores que influyen en la calibración del mismo. Por ende, así se investigaron todas las variables predominantes en el equipo de medición, en el mensurando, en las condiciones ambientales y en la pericia del operario. El resultado fue un modelo matemático asistido por computadora, basado en la serie de Taylor, que permite calcular la incertidumbre de medición de la fuerza de frenado, y a la cual se le puede cargar las mediciones realizadas durante la calibración de un frenómetro en el Taller de Revisión Técnica Vehicular (TRTV

VET&PHARM pilot project: A case study focusing on the perceived value of a pilot seminar on interprofessional communication for students in Pharmacy and Veterinary Medicine in the rational delivery of drugs for veterinary use

Antibiotic resistance has gained attention, prompting changes in prescribing practices in human and veterinary medicine. However, this hasn't extended to over-the-counter drugs like antiparasitic medications. The current public health landscape emphasizes interdisciplinary approaches in healthcare. Strengthening community pharmacists' proficiency in veterinary pharmacotherapy is crucial, especially in addressing antiparasitic resistance and environmental impact. The VET&PHARM pilot project aimed to evaluate the perceived value of an interdisciplinary healthcare simulation in education, involving pharmacy and veterinary students. Methods: This study assessed the perceived value of an interdisciplinary healthcare simulation focusing on rational pharmacy-based veterinary drug dispensing. Students engaged in role-playing, simulating the rational delivery of veterinary medications at an educational pharmacy counter. Scenarios included a pharmacist receiving a veterinary drug request from a pet owner. Unable to fulfill it without consulting a veterinarian, a telemedicine sequence ensued between the pharmacist and the vet. Debriefings emphasized professionals' actions and their impact on patient and animal care. The activity, with 40 student volunteers, featured two VET&PHARM sessions in May 2022. Post-activity, an online questionnaire assessed perceived value for both pharmacy and veterinary students. Results: With an 82.5% response rate, post-intervention questionnaires highlighted the educational interest of the pilot seminar. Students reported improved collaboration and confidence in providing veterinary medicine services and counseling. Discussion and Conclusion: Beyond developing communication and collaboration skills, students exceeded expectations, embracing the One Health vision. This initiative addresses the urgent need for improved rational dispensing practices in veterinary medicines, contributing to the overarching goal of enhancing healthcare delivery in pharmacy.15. Life on lan

Vegetative endocarditis in equids (1994--2006).

peer reviewedBACKGROUND: Endocarditis is a rare heart condition with variable clinical expressions in equids. Risk factors for this disease are incompletely understood. OBJECTIVE: Describe risk factors for endocarditis in equids. ANIMALS: One hundred and fifty-three equids admitted to Liège University, 9 diagnosed with endocarditis and 144 free from endocarditis but admitted to the hospital with a differential diagnosis including this disease. METHODS: Retrospective case-control study. RESULTS: Equids with endocarditis were significantly younger (mean age = 4.84 +/- 5.74 years) than control equids (mean age = 10.8 +/- 7.73 years) (P = .01). No sex or breed predisposition was observed. Animals with hyperthermia (odds ratio [OR] = 24.4; confidence interval [CI] = 1.40-428), synovial distension (OR = 13.4; CI = 3.00-59.8), lameness (OR = 6.52; CI = 1.63-26.1), hyperglobulinemia (OR = 26.4; CI = 3.03-229), hypoalbuminemia (OR = 11.4; CI = 1.34-96.8), hyperfibrinogenemia (OR = 9.81; CI = 1.16-82.7), or leukocytosis (OR = 7.12; CI = 1.40-36.4) presented a significantly higher risk of having endocarditis than control horses. The presence of two of the clinical signs mentioned above significantly increased the probability of a diagnosis of endocarditis (P< or = .05). CONCLUSIONS AND CLINICAL IMPORTANCE: Age is associated with equine endocarditis. The diagnostic value of certain clinical signs and abnormalities in blood parameters in this disease are described

Teaching one health: Animal husbandry in a post-graduate interdisciplinary curriculum

peer reviewe

Learning to Look -Purpose and Design of an Awareness- Raising Online Course in Veterinary Sciences

Abstract. This paper reports on a work in progress: an online self-instruction course created to stimulate students&apos; awareness processes when dealing with pictures. Using non-clinical material, the &quot;Learning to Look&quot; course was designed as a preliminary training to the observation of histological sections, radiological graphs, and other specialized visual material. Following a presentation of the project, salient results of a feedback questionnaire completed by 382 students about their experience of the course are provided

Mucins and Pathogenic Mucin-Like Molecules Are Immunomodulators During Infection and Targets for Diagnostics and Vaccines

Mucins and mucin-like molecules are highly O-glycosylated proteins present on the cell surface of mammals and other organisms. These glycoproteins are highly diverse in the apoprotein and glycan cores and play a central role in many biological processes and diseases. Mucins are the most abundant macromolecules in mucus and are responsible for its biochemical and biophysical properties. Mucin-like molecules cover various protozoan parasites, fungi and viruses. In humans, modifications in mucin glycosylation are associated with tumors in epithelial tissue. These modifications allow the distinction between normal and abnormal cell conditions and represent important targets for vaccine development against some cancers. Mucins and mucin-like molecules derived from pathogens are potential diagnostic markers and targets for therapeutic agents. In this review, we summarize the distribution, structure, role as immunomodulators, and the correlation of human mucins with diseases and perform a comparative analysis of mucins with mucin-like molecules present in human pathogens. Furthermore, we review the methods to produce pathogenic and human mucins using chemical synthesis and expression systems. Finally, we present applications of mucin-like molecules in diagnosis and prevention of relevant human diseases

Heme Drives Susceptibility of Glomerular Endothelium to Complement Overactivation Due to Inefficient Upregulation of Heme Oxygenase-1

Atypical hemolytic uremic syndrome (aHUS) is a severe disease characterized by microvascular endothelial cell (EC) lesions leading to thrombi formation, mechanical hemolysis and organ failure, predominantly renal. Complement system overactivation is a hallmark of aHUS. To investigate this selective susceptibility of the microvascular renal endothelium to complement attack and thrombotic microangiopathic lesions, we compared complement and cyto-protection markers on EC, from different vascular beds, in in vitro and in vivo models as well as in patients. No difference was observed for complement deposits or expression of complement and coagulation regulators between macrovascular and microvascular EC, either at resting state or after inflammatory challenge. After prolonged exposure to hemolysis-derived heme, higher C3 deposits were found on glomerular EC, in vitro and in vivo, compared with other EC in culture and in mice organs (liver, skin, brain, lungs and heart). This could be explained by a reduced complement regulation capacity due to weaker binding of Factor H and inefficient upregulation of thrombomodulin (TM). Microvascular EC also failed to upregulate the cytoprotective heme-degrading enzyme heme-oxygenase 1 (HO-1), normally induced by hemolysis products. Only HUVEC (Human Umbilical Vein EC) developed adaptation to heme, which was lost after inhibition of HO-1 activity. Interestingly, the expression of KLF2 and KLF4—known transcription factors of TM, also described as possible transcription modulators of HO-1- was weaker in micro than macrovascular EC under hemolytic conditions. Our results show that the microvascular EC, and especially glomerular EC, fail to adapt to the stress imposed by hemolysis and acquire a pro-coagulant and complement-activating phenotype. Together, these findings indicate that the vulnerability of glomerular EC to hemolysis is a key factor in aHUS, amplifying complement overactivation and thrombotic microangiopathic lesions

Deciphering cell-herpesvirus interactions using force-distance curve-based atomic force microscopy

Viruses are the most numerous species on earth. Some of them can be the cause of mild to severe diseases with potential deadly outcomes. Viral infection is initiated by the attachment of virus particles to the surface of target host cells. Understanding the molecular interactions taking place between virus particles and cell surface molecules is of fundamental interest for the development of new therapeutics interfering with virus attachment processes. Most of the insights gained on virus-cell interactions are currently obtained via ensemble molecular studies providing average responses of populations of virions. To unveil molecular details arising from biological variability, studies performed at the single virus particle-level could provide new valuable insights into virus attachment mechanisms. The aim of this PhD thesis is therefore to implement new tools to decipher the putative roles of viral surface glycoproteins upon attachment to cell surfaces at the single-virus particle level. This work involves the use and development of atomic force microscopy-based single-virus force spectroscopy (SVFS) to decipher the molecular mechanisms of herpesvirus attachment to cell surface glycosaminoglycans (GAGs). We investigated the attachment properties of single virions of Murid Herpesvirus-4 (MuHV-4) and Herpes Simplex Virus-1 (HSV-1) towards both purified GAGs grafted onto inert surfaces and GAGs on living cell surfaces. We observed that both glycoprotein gp150 (MuHV-4) and the mucin-like region of glycoprotein gC (HSV-1) play regulatory roles upon attachment of virions to GAGs, by regulating the multivalency of other viral glycoprotein-GAG interactions. We further deciphered the putative roles of two apparently redundant glycoproteins of MuHV-4: gH/gL and gp70. We showed that gH/gL provides a larger contribution to virus-GAG interactions than gp70 and that the gH/gL-GAG interactions have a higher inherent stability. The molecular insights gained by SVFS on the complex mechanisms developed by herpesviruses to attach target cells could contribute to the development of new antiviral therapeutics potentially targeting specific viral surface glycoproteins.(AGRO - Sciences agronomiques et ingénierie biologique) -- UCL, 202