Identification of a superagonist variant of the immunodominant Yellow fever virus epitope NS4b214-222 by combinatorial peptide library screening

The CD8 T cell response to the HLA-A2-restricted epitope LLWNGPMAV (LLW) of the non-structural protein 4b of Yellow Fever Virus (YFV) is remarkably immunodominant, highly prevalent and powerful in YFV-vaccinated humans. Here we used a combinatorial peptide library screening in the context of an A2/LLW-specific CD8 T cell clone to identify a superagonist that features a methionine to isoleucine substitution at position 7. Based on in silico modeling, the functional enhancement of this LLW-7I mutation was associated with alterations in the structural dynamics of the peptide in the major histocompatibility complex (pMHC) binding with the T cell receptor (TCR). While the TCR off-rate of LLW-7I pMHC is comparable to the wild type peptide, the rigidity of the 7I peptide seems to confer less entropy loss upon TCR binding. This LLW-7I superagonist is an example of improved functionality in human CD8 T cells associated with optimized ligand rigidity for TCR binding and not with changes in TCR:pMHC off-rate kinetics

Agrobacterium-mediated transformation of Asparagus officinalis L. long-term embryogenic callus and regeneration of transgenic plants

Twenty-three independent kanamycin resistant lines were obtained after cocultivation of longterm embryogenic cultures of three Asparagus officinalis L. genotypes with an Agrobacterium tumefaciens strain harboring ß-glucuronidase and neomycin phosphotransferase II genes. All the lines showed ß-glucuronidase activity by histological staining. DNA analysis by Southern blots of the kanamycin resistant embryogenic lines and of a plant regenerated from one of them confirmed the integration of the T-DNA

Evolution of obsessive and compulsive symptoms over two years of deep brain stimulation cessation and explantation, after fourteen years of continuous stimulation for treatment-resistant obsessive-compulsive disorder. A case report

International audienc

Identification of a superagonist variant of the immunodominant Yellow fever virus epitope NS4b214-222 by combinatorial peptide library screening

The CD8 T cell response to the HLA-A2-restricted epitope LLWNGPMAV (LLW) of the non-structural protein 4b of Yellow Fever Virus (YFV) is remarkably immunodominant, highly prevalent and powerful in YFV-vaccinated humans. Here we used a combinatorial peptide library screening in the context of an A2/LLW-specific CD8 T cell clone to identify a superagonist that features a methionine to isoleucine substitution at position 7. Based on in silico modeling, the functional enhancement of this LLW-7I mutation was associated with alterations in the structural dynamics of the peptide in the major histocompatibility complex (pMHC) binding with the T cell receptor (TCR). While the TCR off-rate of LLW-7I pMHC is comparable to the wild type peptide, the rigidity of the 7I peptide seems to confer less entropy loss upon TCR binding. This LLW-7I superagonist is an example of improved functionality in human CD8 T cells associated with optimized ligand rigidity for TCR binding and not with changes in TCR:pMHC off-rate kinetics

Les « chrétiens modérés » en France et en Europe (1870-1960)

L'étude des modérés dans la vie politique française, et plus encore, celle du poids des chrétiens modérés, a longtemps constitué un point aveugle de l'historiographie française. Un important colloque tenu à Grenoble, il y a plus de quarante ans, et un récent colloque tenu à Nancy, il y cinq ans, constituent encore les ouvrages de référence. C'est à combler ce trou que voudrait travailler ce livre. L’effort des historiens qui ont contribué à cet ouvrage, fruit d’un colloque tenu à Lille en 2005 et 2006, se résume à trois apports essentiels. Premier apport : la contribution d’éminents historiens, spécialistes des pays européens, dans lesquels les relations entre philosophie politique et théologie n’ont pas fait l’objet de la même réserve qu’en France, a permis de mieux cerner la singularité de l’historiographie française. Deuxième apport : les historiens spécialistes des rapports mouvementés entre l’Église et la République ont confirmé le rôle souvent décisif de ces catholiques modérés dans la fondation et la stabilisation du régime républicain, confirmant le poids de ces « catholiques selon le suffrage universel » chers à Littré. Ils ont également souligné l’apport de ces derniers dans la refondation de la République aux lendemains de la Libération. Troisième apport et non des moindres : essayer de faire comprendre comment s’articule dans la conscience de l’individu-acteur l’option d’ordre proprement religieuse, philosophique et théologique, et le choix dans l’ordre politique