Forensic exploitation of patterned injuries: Promoting structured analysis as an early assessment for comparison process.

Practice at our Center shows that approach using 3D surface imaging for morphometric comparison of patterned injuries does not always lead to accurate conclusions. We decided to evaluate whether a selection protocol focused on analysis phase could enable us to form an early assessment of the outcome of a comparison process, and then to select lesions likely to lead to a probative conclusion. 23 blunt objects were used to create 65 patterned injuries on an experimental model simulating human skin. A blinded analysis and a comparison were conducted on photographs and 3D models of the lesions. Statement of analysis phase was consistent with comparison results in most cases, enabling correct identification of the responsible object or at least keeping it as possibly responsible among 2 to 3 objects. Our protocol has been demonstrated to improve ability to exploit patterned injuries from surface imaging, despite certain limiting factors

Ok Google, Start a Fire. IoT devices as witnesses and actors in fire investigations.

Fire incidents are amongst the most destructive events an investigator might encounter, completely transforming a scene with most of the objects left in ashes or highly damaged. Until now, fire investigations relied heavily on burn patterns and electrical artifacts to find possible starting locations, as well as witness statements and more recently witness imagery. As Internet of Things (IoT) devices, often seen as connected smart devices, become more common, the various sensors embedded within them provide a novel source of traces about the environment and events within. They collect and store information in different locations, often not touched by the event, such as remote servers (cloud) or companion smartphones, widening the investigation field for fire incidents. This work presents two controlled fire incidents in apartments that we furnished, equipped with IoT devices, and subsequently burnt. We studied the traces retrievable from the objects themselves after the incident, the companion smartphone apps, and the cloud and assessed the value of the information they conveyed. This research highlighted the pertinence to consider traces from IoT devices in the forensic process of fire investigation

An exploratory study toward the contribution of 3D surface scanning for association of an injury with its causing instrument.

3D surface scanning is a technique brought forward for wound documentation and analysis in order to identify injury-causing tools in legal medicine and forensic science. Although many case reports have been published, little is known about the methodology employed by the authors. The study reported here is exploratory in nature, and its main purpose was to get a first evaluation of the ability of an operator, by means of 3D surface scanning and following a simple methodology, to correctly exclude or associate an incriminated tool as the source of a mock wound. Based on these results, an assessment of the possibility to define a structured methodology that could be suitable for this use was proposed. Blunt tools were used to produce 'wounds' on watermelons. Both wounds and tools were scanned with a non-contact optical surface 3D digitising system. Analysis of the obtained 3D models of wounds and tools was undertaken separately. This analytical phase was followed by a qualitative and a quantitative comparison. Results showed that in more than half of the cases, we obtained a correct association but the prevalence of wrong association was still high due to mark deformation and other limitations. Even if the findings of this exploratory study cannot be generalised, they suggest that the simple and direct comparison process is not reliable enough for a systematic routine application. The article highlights the importance of an analysis phase preceding the comparison step. Limitations of the technique, ensuring needs and possible paths for improvement are also expounded

Investigating the availability of 3D-printed firearm designs on the clear web.

The release of the plans of the 3D-printed Liberator firearm sparked a wave of new designs from creators worldwide, resulting in an extensive collection of 3D-printed firearm plans, in particular blueprints, and parts available for almost unrestricted download on the internet. Identifying and categorizing the diverse range of 3D-printed firearms and components pose a challenge due to the abundance of designs available. Between 2021 and April 2023, data was collected on over 2,100 3D-printed firearm plans. While blueprints of fully 3D-printed firearms initially dominated the scene, hybrid designs and parts kit completions / conversions (PKC) have gained popularity for their improved reliability and performance. The now highly networked community offers considerable support with detailed instructions and procedures, providing precise guidance for construction. This systematic classification, grouping and structuration of the recorded data on the Clear Web supported the identification of patterns of the main threat trends related to 3D-printed firearms

Cleaning deteriorated elements of ammunition: Development of a procedure applied to cartridge cases from the Second World War.

Deteriorated elements of ammunition can be found while investigating different types of events. Exposure to adverse environmental conditions may lead to metal alteration (corrosion) or organic material deposition (contaminations) on the exposed elements of ammunition. From a forensic perspective, both types of deterioration pose challenges when observing marks left by the firearms used to discharge the corresponding ammunition (e.g. firing pin, extractor). The longer the time of exposure to the adverse environmental conditions, the more challenging the observation of such marks. A literature review highlighted three previously published restorative methods used to clean deteriorated elements of ammunition. The aim of this research is to develop a cleaning procedure applicable to cartridge cases exposed to adverse environmental conditions, while avoiding the degradation of marks left by the firearms used to discharge the corresponding ammunition. A first batch of 21 brass cartridge cases dating back to the Second World War (WWII) was used to develop a cleaning sequence involving the three methods. The efficiency of each restorative method was qualitatively assessed using optical macroscopy and the Evofinder® ballistic identification system. The developed sequence relies on successive applications of Tickopur® TR 7 (a diluted soft metal cleaner), sulfuric acid and finally Aqua Regia (HCl 37% and HNO <sub>3</sub> 75%), all of them involving ultrasonic baths. The resulting cleaning sequence was subsequently applied to three batches of Second World War cartridge cases discovered in France and Russia. This sequential procedure allows the effective cleaning of WWII brass cartridge cases while highlighting different marks left by firing pins, extractors, ejectors, and breech faces. Applying a forensic analysis and comparison process to the marks highlighted on these elements of ammunition can support the verification of historical facts when reconstructing events which took place more than seventy years ago

Touch DNA collection - Performance of four different swabs.

A collaborative study conducted by three police forensic units, a DNA laboratory, and a forensic academic institute was undertaken in order to compare the performance of four different swabs in controlled and quasi-operational conditions. For this purpose, a reference swab (Prionics cardboard evidence collection kit) currently used within the police forensic units and 3 challenger swabs (COPAN 4N6FLOQSwabs™ (Genetics variety), Puritan FAB-MINI-AP and Sarstedt Forensic Swab) were used for collecting DNA traces from previously used items (referred as "touch DNA" in this article) including on 60 collars, 60 screwdrivers and 60 steering wheels obtained from volunteers. For each comparison, the surface considered was divided into two equal components; one was sampled with the reference swab and the other with one of the three challenger swabs. This lead to a total of 360 samples. Conclusions were consistent within the four operational partners. From a practical point of view, the COPAN 4N6FLOQSwabs™ (Genetics variety) was judged the most convenient to use. Furthermore, it allowed the recovery of significantly more DNA from collars (0.65 vs 0.13 ng/μL) and steering wheels (2.82 vs 1.77 ng/μL), and a similar amount of DNA from screwdrivers (0.032 vs 0.026 ng/μL) compared with the Prionics reference swab. The two other challenger swabs provided results that were not significantly different from the reference swab, except for the Puritan swab, whose performance was significantly lower for steering wheels (0.37 vs 0.58 ng/μL). As part of a conservation study, 50 μL of a blood dilution (1/4 with PBS) was deposited on a total of 105 COPAN (Genetics and Crime Scene varieties), Prionics and Sarstedt swabs. They were stored within a cupboard at room temperature. The integrity of the recovered DNA was evaluated with NGM SElect™ DNA profiles after different time-spans ranging from 1 day to 12 months by comparing the height difference of the peaks occurring at the shortest and longest loci, respectively. DNA seemed to remain stable, except when using the COPAN 4N6FLOQSwabs™ treated with an antimicrobial agent (Crime scene variety), which resulted in significant DNA degradation. Following these tests, the COPAN 4N6FLOQSwabs™ (Genetics variety), a model with a desiccant, was selected for further testing in fully operational conditions

Contribution of isotope ratio mass spectrometry to the investigation of improvised explosives: isotopic study of black powders ans ammonium nitrates

The establishment of legislative rules about explosives in the eighties has reduced the illicit use of military and civilian explosives. However, bomb-makers have rapidly taken advantage of substances easily accessible and intended for licit uses to produce their own explosives. This change in strategy has given rise to an increase of improvised explosive charges, which is moreover assisted by the ease of implementation of the recipes, widely available through open sources. While the nature of the explosive charges has evolved, instrumental methods currently used in routine, although more sensitive than before, have a limited power of discrimination and allow mostly the determination of the chemical nature of the substance. Isotope ratio mass spectrometry (IRMS) has been applied to a wide range of forensic materials. Conclusions drawn from the majority of the studies stress its high power of discrimination. Preliminary studies conducted so far on the isotopic analysis of intact explosives (pre-blast) have shown that samples with the same chemical composition and coming from different sources could be differentiated. The measurement of stable isotope ratios appears therefore as a new and remarkable analytical tool for the discrimination or the identification of a substance with a definite source. However, much research is still needed to assess the validity of the results in order to use them either in an operational prospect or in court. Through the isotopic study of black powders and ammonium nitrates, this research aims at evaluating the contribution of isotope ratio mass spectrometry to the investigation of explosives, both from a pre-blast and from a post-blast approach. More specifically, the goal of the research is to provide additional elements necessary to a valid interpretation of the results, when used in explosives investigation. This work includes a fundamental study on the variability of the isotopic profile of black powder and ammonium nitrate in both space and time. On one hand, the inter-variability between manufacturers and, particularly, the intra-variability within a manufacturer has been studied. On the other hand, the stability of the isotopic profile over time has been evaluated through the aging of these substances exposed to different environmental conditions. The second part of this project considers the applicability of this high-precision technology to traces and residues of explosives, taking account of the characteristics specific to the field, including their sampling, a probable isotopic fractionation during the explosion, and the interferences with the matrix of the site

Extraction and concentration of vapors from fire debris for forensic purposes: evaluation of the use of Radiello Passive Air Sampler

The Radiello Passive Air Sampler is one of the latest innovations developed for the sampling of pollutants in the air by passive headspace. It has been reported that its properties allow an enhanced sensitivity, reproducibility and adsorption capacity. It therefore appears to be of interest in the extraction of potential residues of ignitable liquids present in fire debris when arson is suspected. A theoretical approach and several laboratory tests have made it possible to precisely characterize in a forensic perspective the potential of the device in extracting and concentrating the vapors of ignitable liquids found in fire debris. Despite some advantages, the Radiello device appears to be less efficient than traditional axial symmetry samplers