POSSIBLE ROLE OF NITRIC OXIDE IN REGULATION OF MICROCIRCULATION IN THE ORAL CAVITY TISSUES OF RAT

Possible role of Nitric oxide (NO) in terms formation of basal blood flow level in oral cavity tissue is studied using the method of hydrogen electrochemical generation in anesthetized rats.It was found that NO plays a leading role in oral mucosa and submandibular gland basal blood flow regulation and does not have any significant effect on blood circulation of the lower snout tissue.Possible role of Nitric oxide (NO) in terms formation of basal blood flow level in oral cavity tissue is studied using the method of hydrogen electrochemical generation in anesthetized rats.It was found that NO plays a leading role in oral mucosa and submandibular gland basal blood flow regulation and does not have any significant effect on blood circulation of the lower snout tissue

DEVELOPMENT OF ANALYTICAL PROCEDURE FOR THE QUANTITATIVE VALIDATION OF BRONCHOSPASMOLITIC THICK EXTRACT FROM CAUCASIAN IVY

'Kavsuron' is a para-medical bronchospasmolitic preparation (on the base of thick extract) from the leaves of Hedera helix L. seu H. Caucasigena Pojark., proposed as an expectorant and bronchospasmolytic remedy for adults and in pediatry. As the hederasaponin C (HSC) is a chemical and biological marker of ivy, the simple and reliable HPLC method is developed for its quantitative evaluation in the thick extract. The UV detection is performed at 207 nm. The proposed HPLC method is linear in the range studied (r2>0.999) for all the analytes. The method is precise with intra- and inter-day variations of less than 2.0%. The mean recoveries of the analytes range from 96.7 to 99.2%. The results indicated that developed HPLC method could be used for the quality control of 'Kavsuron'

Development of analytical procedure for the quantitative validation of bronchospasmolitic thick extract from caucasian ivy

'Kavsuron' is a para-medical bronchospasmolitic preparation (on the base of thick extract) from the leaves of Hedera helix L. seu H. Caucasigena Pojark., proposed as an expectorant and bronchospasmolytic remedy for adults and in pediatry. As the hederasaponin C (HSC) is a chemical and biological marker of ivy, the simple and reliable HPLC method is developed for its quantitative evaluation in the thick extract. The UV detection is performed at 207 nm. The proposed HPLC method is linear in the range studied (r2>0.999) for all the analytes. The method is precise with intra- and inter-day variations of less than 2.0%. The mean recoveries of the analytes range from 96.7 to 99.2%. The results indicated that developed HPLC method could be used for the quality control of 'Kavsuron'

Ivy water extracts as gastric ulcer preventive agents

In folk medicine the ivies (Hedera L. Fam.Araliaceae) are known as plants possessing diverse curative properties. A comparative phytochemical study of the biologically active water extracts of H. colchica and H. helix and evaluation of their ulcer preventive efficacy in ethanol-induced ulcer model in rats was carried out. Water extracts of H. colchica and H. helix (300 mg/kg, i.p.) significantly (p<0.01) decrease the ulcer index (0.50 and 1.38 vs 3.17 in control) and rise macroscopic curative ratio (84.2% and 56.6%, respectively). The results clearly indicate that pretreatment with water extract of H. colchica is preferable and further experiments are required to isolate the active principals responsible for itsantiulcerogenic activity

A new triterpene glycoside from fruit of Phytolacca americana

Glycosides H and I, the structures of which were established by modern physicochemical analytical methods (PMR, 13C NMR, COSY, TOCSY, HMBC, MS) and acid-base hydrolysis, were isolated from the purified total saponins from fruit of Phytolacca americana containing at least 10 triterpene glycosides by rechromatography of enriched fractions over a column of silica gel. Glycoside H was a bidesmoside of phytolaccageninic acid, which was isolated earlier from cell culture of Phytolacca acinosa. Glycoside I was 3-O-(β-D-xylopyranosyl-(1 → 3)-β-D-galactopyranosyl-(1 → 3)- β -D-xylopyranosyl)-28-O- β -D-glucopyranosyl phytolaccagenin, which was isolated by us for the first time

Possible Role of Nitric Oxide in Regulation of Microcirculation in the Oral Cavity Tissues of Rat

Possible role of Nitric oxide (NO) in terms formation of basal blood flow level in oral cavity tissue is studied using the method of hydrogen electrochemical generation in anesthetized rats.It was found that NO plays a leading role in oral mucosa and submandibular gland basal blood flow regulation and does not have any significant effect on blood circulation of the lower snout tissue

Evaluation of the mutagenic and antimutagenic activities of saponins from Hedera colchica K. Koch

Note portant sur l'auteur Note portant sur l'auteur Note portant sur l'auteur Note portant sur l'auteur Note portant sur l'auteur Note portant sur l'auteur Note portant sur l'auteur Introduction Hedera colchica K. Kock. (Araliaceae) is an endemic plant in Georgia. The leaves of this plant are used in traditional medicine as bronchospasmolitic, secretolytic and anti-inflammatory remedies. (Sakartvelos Floras, 1984; Rastitllnie and col., 1988) The mutagenic and antimutagenic activities of 7 mon..

Antileishmanial activity of three saponins isolated from ivy, α- hederin, β-hederin and hederacolchiside A1, as compared to their action on mammalian cells cultured in vitro

The in vitro antileishmanial activity of three saponins isolated from ivy, α-hederin, β-hederin and hederacolchiside A1, was investigated on Leishmania infantum. The assessment of possible targets (membrane integrity, membrane potential, DNA synthesis and protein content) was performed in both Leishmania promastigotes and human monocytes (THP1 cells). Results observed in Leishmania showed that the saponins exhibited a strong antiproliferative activity on all stages of development of the parasite by altering membrane integrity and potential: hederacolchiside A1 appeared to be the most active compound against both promastigotes and amastigotes. Results observed in THP1 cells demonstrated that the saponins exerted also a potent antiproliferative activity against human monocytes, by producing a significant DNA synthesis inhibition. The ratio between antileishmanial activity on amastigotes and toxicity to human cells suggested that the saponins could be considered as possible antileishmanial drugs