Increased expression of CD154 and FAS in SLE patients' lymphocytes

An increased level of apoptotic material and B cell activation leading to autoantibody production are hallmarks of systemic lupus erythematoses (SLE). Increased FAS expression, apoptosis, and CD154-mediated signaling, enabling T-B cell interaction are involved in the pathogenesis of SLE. This study addresses the expression profile of CD154 and FAS in the peripheral blood of patients with SLE, rheumatoid arthritis (RA) and normal healthy control donors. Surface markers on peripheral blood T and B cells from patients and healthy control donors were assessed using flow cytometry. The expression of CD154 and FAS were significantly increased in T and B cells of SLE patients as compared to healthy control donors and RA patients. In SLE and RA patients, FAS expression strongly correlated with CD154 expression on T cells, which was not found in healthy control donors. FAS expression was also associated with the occurrence of anti-DNA antibodies. We demonstrate high CD154 and FAS expression as a characteristic feature of SLE. This pattern may reflect simultaneous activation of apoptosis and activation of B-T cell interaction in SL

SCREENINGUL BOLII CELIACE ÎN HEPATITELE CRONICE VIRALE C

Înţelegerea relaţiei dintre agenţii infecţioşi şi autoimunitate pentru diagnosticarea precoce şi, de asemenea, prevenirea bolii celiace, precum şi a implicaţiilor clinice ale acesteia în cursul tratamentului cu interferon

The importance of determining human leucocyte antigens in preventing intestinal lymphoma in patients with celiac disease

Identification of celiac disease, by determining human leucocyte antigens DQ2/DQ8, is important since recent long-term studies have shown that the mortality of celiac disease is increased, if it is unrecognized and untreated. In this sense, we wanted to see the usefulness of genetic tests in celiac disease diagnosis and screening. Material and methods. During 2010 we determined by PCR, DQ2/DQ8 haplotype, in a group of 27 children with celiac disease and 9 of their brothers, serologically negative for celiac disease. Results. 22 children and 7 of their brothers confirmed the diagnosis of celiac disease, DR3-DQ2 haplotype was predominant in children with celiac disease and DR4-DQ8 to their brothers. Conclusions. Genetic testing to determine human lecocyte antigens remain the most reliable test in the diagnosis of celiac disease but also in identifying family risk for people with celiac disease