99 research outputs found
Spectrophotometric determination of oils with radicals of conjugated octadecatrienoic acids
An approach specific for triacylglycerides (TAGs) is proposed for chromatographic separation of compounds with different chromophores using spectrophotometric detection instead of preliminary calibration of the detector using unavailable (or difficultly accessible) reference standard
ΠΠ ΠΠΠΠΠΠ« ΠΠΠΠΠ’ΠΠ€ΠΠΠΠ¦ΠΠ ΠΠ‘ΠΠΠ’ΠΠ€ΠΠΠΠΠ ΠΠΠΠΠ‘Π’ΠΠΠ lilium pumilum l ΠΠ Π ΠΠ ΠΠΠΠΠΠΠΠ ΠΠΠ’ΠΠΠ ΠΠΠ ΠΠ©ΠΠΠΠ-Π€ΠΠΠΠΠΠ ΠΠΠΠ₯ Π‘Π Π‘ΠΠΠΠ’Π ΠΠ€ΠΠ’ΠΠΠΠ’Π ΠΠ§ΠΠ‘ΠΠΠ Π ΠΠΠ‘Π‘-Π‘ΠΠΠΠ’Π ΠΠΠΠ’Π ΠΠ§ΠΠ‘ΠΠΠ ΠΠΠ’ΠΠΠ’ΠΠ ΠΠΠΠΠΠΠ
With the use of the reversed-phase high-performance liquid chromatography (HPLC) with diode-array and mass spectrometric detection, the species composition of xanthophylls of dried red color Lilium pumilum Delile flowers was determined. It was established that this plant was a unique source of capsorubin derivatives. The main components of the carotenoid complex were diesters of all-trans and cis-isomers of capsorubin (about 70 mol %) formed by the saturated fatty acids from capric to stearic with the largest contribution of myristic (~ 36 mol %) and lauric (~ 31 mol %) acids. In addition to diethers in the extracts, monoesters of capsorubin and capsanthin were also found (about 30 % of the peak areas). The possibilities of varying the composition of the mobile phase - acetone and propanol-2 as components with the high eluting capacity and acetonitrile and methanol as components with the lower eluting power - for the separation of diethers and differentiation of derivatives of capsanthin and capsorubin and to prevent the imposition of peaks of two series homologues were considered. The features of ion formation during the mass spectrometric detection in the mode of chemical ionization at the atmospheric pressure (APCI) were determined. The possibility of using the parameters of capsorubin diester retention to determine the thermodynamically substantiated dead time of the column was considered.Key words: capsorubin, capsanthin, esters, Lilium pumilum Delile, RP-HPLC, APCI, separation selectivity(Russian)Β DOI: http://dx.doi.org/10.15826/analitika.2019.23.4.002V.I. Deineka, T.G. Burzhynskaya, L.A. DeinekaΒ Belgorod State National Research University, Russian Federation, 308015, Belgorod, ul. Pobedy, 85ΠΡΠ΅Π΄Π»ΠΎΠΆΠ΅Π½ ΡΠΏΠΎΡΠΎΠ± ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΡ Π²ΠΈΠ΄ΠΎΠ²ΠΎΠ³ΠΎ ΡΠΎΡΡΠ°Π²Π° ΠΊΡΠ°Π½ΡΠΎΡΠΈΠ»Π»ΠΎΠ² Π²ΡΡΡΡΠ΅Π½Π½ΡΡ
ΡΠ²Π΅ΡΠΊΠΎΠ² ΠΊΠ°ΡΠ»ΠΈΠΊΠΎΠ²ΠΎΠΉ Π»ΠΈΠ»ΠΈΠΈ Lilium pumilum Delile ΠΊΡΠ°ΡΠ½ΠΎΠ³ΠΎ ΡΠ²Π΅ΡΠ° Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ ΠΎΠ±ΡΠ°ΡΠ΅Π½Π½ΠΎ-ΡΠ°Π·ΠΎΠ²ΠΎΠΉ Π²ΡΡΠΎΠΊΠΎΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΠΉ ΠΆΠΈΠ΄ΠΊΠΎΡΡΠ½ΠΎΠΉ Ρ
ΡΠΎΠΌΠ°ΡΠΎΠ³ΡΠ°ΡΠΈΠΈ Ρ Π΄ΠΈΠΎΠ΄Π½ΠΎ-ΠΌΠ°ΡΡΠΈΡΠ½ΡΠΌ ΠΈ ΠΌΠ°ΡΡ-ΡΠΏΠ΅ΠΊΡΡΠΎΠΌΠ΅ΡΡΠΈΡΠ΅ΡΠΊΠΈΠΌ Π΄Π΅ΡΠ΅ΠΊΡΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ. Π£ΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ, ΡΡΠΎ Π΄Π°Π½Π½ΠΎΠ΅ ΡΠ°ΡΡΠ΅Π½ΠΈΠ΅ ΡΠ²Π»ΡΠ΅ΡΡΡ ΡΠ½ΠΈΠΊΠ°Π»ΡΠ½ΡΠΌ ΡΠ°ΡΡΠΈΡΠ΅Π»ΡΠ½ΡΠΌ ΠΈΡΡΠΎΡΠ½ΠΈΠΊΠΎΠΌ ΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄Π½ΡΡ
ΠΊΠ°ΠΏΡΠΎΡΡΠ±ΠΈΠ½Π°. ΠΡΠ½ΠΎΠ²Π½ΡΠΌΠΈ ΠΊΠΎΠΌΠΏΠΎΠ½Π΅Π½ΡΠ°ΠΌΠΈ ΠΊΠ°ΡΠΎΡΠΈΠ½ΠΎΠΈΠ΄Π½ΠΎΠ³ΠΎ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠ° ΡΠ²Π»ΡΡΡΡΡ Π΄ΠΈΡΡΠΈΡΡ ΠΏΠΎΠ»Π½ΠΎΡΡΡΡ ΡΡΠ°Π½Ρ- ΠΈ ΡΠΈΡ-ΠΈΠ·ΠΎΠΌΠ΅ΡΠΎΠ² ΠΊΠ°ΠΏΡΠΎΡΡΠ±ΠΈΠ½Π° (ΠΎΠΊΠΎΠ»ΠΎ 70 ΠΌΠΎΠ»Ρ %), ΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½Π½ΡΠ΅ Π½Π°ΡΡΡΠ΅Π½Π½ΡΠΌΠΈ ΠΆΠΈΡΠ½ΡΠΌΠΈ ΠΊΠΈΡΠ»ΠΎΡΠ°ΠΌΠΈ ΠΎΡ ΠΊΠ°ΠΏΡΠΈΠ½ΠΎΠ²ΠΎΠΉ Π΄ΠΎ ΡΡΠ΅Π°ΡΠΈΠ½ΠΎΠ²ΠΎΠΉ Ρ Π½Π°ΠΈΠ±ΠΎΠ»ΡΡΠΈΠΌ Π²ΠΊΠ»Π°Π΄ΠΎΠΌ ΠΌΠΈΡΠΈΡΡΠΈΠ½ΠΎΠ²ΠΎΠΉ (~ 36 ΠΌΠΎΠ»Ρ %) ΠΈ Π»Π°ΡΡΠΈΠ½ΠΎΠ²ΠΎΠΉ (~ 31 ΠΌΠΎΠ»Ρ %) ΠΊΠΈΡΠ»ΠΎΡ. ΠΡΠΎΠΌΠ΅ Π΄ΠΈΡΡΠΈΡΠΎΠ² Π² ΡΠΊΡΡΡΠ°ΠΊΡΠ°Ρ
ΠΎΠ±Π½Π°ΡΡΠΆΠ΅Π½Ρ ΠΌΠΎΠ½ΠΎΡΡΠΈΡΡ ΠΊΠ°ΠΏΡΠΎΡΡΠ±ΠΈΠ½Π° ΠΈ ΠΊΠ°ΠΏΡΠ°Π½ΡΠΈΠ½Π° (ΠΎΠΊΠΎΠ»ΠΎ 30 % ΠΏΠΎ ΠΏΠ»ΠΎΡΠ°Π΄ΡΠΌ ΠΏΠΈΠΊΠΎΠ²). ΠΠ°ΡΡΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΡΠΎΡΡΠ°Π²Π° ΠΏΠΎΠ΄Π²ΠΈΠΆΠ½ΠΎΠΉ ΡΠ°Π·Ρ β Π°ΡΠ΅ΡΠΎΠ½Π° ΠΈ ΠΏΡΠΎΠΏΠ°Π½ΠΎΠ»Π°-2 ΠΊΠ°ΠΊ ΠΊΠΎΠΌΠΏΠΎΠ½Π΅Π½ΡΠΎΠ² Ρ Π²ΡΡΠΎΠΊΠΎΠΉ ΡΠ»ΡΠΈΡΡΡΡΠ΅ΠΉ ΡΠΏΠΎΡΠΎΠ±Π½ΠΎΡΡΡΡ ΠΈ Π°ΡΠ΅ΡΠΎΠ½ΠΈΡΡΠΈΠ»Π° ΠΈ ΠΌΠ΅ΡΠ°Π½ΠΎΠ»Π° ΠΊΠ°ΠΊ ΠΊΠΎΠΌΠΏΠΎΠ½Π΅Π½ΡΠΎΠ² Ρ ΠΌΠ΅Π½ΡΡΠ΅ΠΉ ΡΠ»ΡΠΈΡΡΡΡΠ΅ΠΉ ΡΠΈΠ»ΠΎΠΉ, β ΠΏΠΎΠ·Π²ΠΎΠ»ΡΠ΅Ρ ΡΠ°Π·Π΄Π΅Π»ΡΡΡ Π΄ΠΈΡΡΠΈΡΡ ΠΈ Π΄ΠΈΡΡΠ΅ΡΠ΅Π½ΡΠΈΡΠΎΠ²Π°ΡΡ ΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄Π½ΡΠ΅ ΠΊΠ°ΠΏΡΠ°Π½ΡΠΈΠ½Π° ΠΈ ΠΊΠ°ΠΏΡΠΎΡΡΠ±ΠΈΠ½Π°, ΠΏΡΠ΅Π΄ΠΎΡΠ²ΡΠ°ΡΠ°Ρ Π½Π°Π»ΠΎΠΆΠ΅Π½ΠΈΡ ΠΏΠΈΠΊΠΎΠ² ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ
Π³ΠΎΠΌΠΎΠ»ΠΎΠ³ΠΎΠ² Π΄Π²ΡΡ
ΡΡΠ΄ΠΎΠ². ΠΡΠΎΠ±Π΅Π½Π½ΠΎΡΡΡΡ ΠΌΠ°ΡΡ-ΡΠΏΠ΅ΠΊΡΡΠΎΠΌΠ΅ΡΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ Π΄Π΅ΡΠ΅ΠΊΡΠΈΡΠΎΠ²Π°Π½ΠΈΡ Π² ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½Π½ΠΎΠΌ ΡΠ΅ΠΆΠΈΠΌΠ΅ Ρ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΈΠΎΠ½ΠΈΠ·Π°ΡΠΈΠΈ ΠΏΡΠΈ Π°ΡΠΌΠΎΡΡΠ΅ΡΠ½ΠΎΠΌ Π΄Π°Π²Π»Π΅Π½ΠΈΠΈ (APCI) ΡΠ²Π»ΡΠ΅ΡΡΡ ΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΠ΅ Π°Π΄Π΄ΡΠΊΡΠΎΠ² Ρ ΠΈΠΎΠ½Π°ΠΌΠΈ Π½Π°ΡΡΠΈΡ. ΠΠΎΠΊΠ°Π·Π°Π½Π° ΠΈ ΠΎΠ±ΠΎΡΠ½ΠΎΠ²Π°Π½Π° Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡΡ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΡ ΠΏΠ°ΡΠ°ΠΌΠ΅ΡΡΠΎΠ² ΡΠ΄Π΅ΡΠΆΠΈΠ²Π°Π½ΠΈΡ Π΄ΠΈΡΡΠΈΡΠΎΠ² ΠΊΠ°ΠΏΡΠΎΡΡΠ±ΠΈΠ½Π° Π΄Π»Ρ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΡ ΠΈΠΌΠ΅ΡΡΠ΅Π³ΠΎ ΡΠ΅ΡΠΌΠΎΠ΄ΠΈΠ½Π°ΠΌΠΈΡΠ΅ΡΠΊΠΈΠΉ ΡΠΌΡΡΠ» ΠΌΠ΅ΡΡΠ²ΠΎΠ³ΠΎ Π²ΡΠ΅ΠΌΠ΅Π½ΠΈ ΠΊΠΎΠ»ΠΎΠ½ΠΊΠΈ.ΠΠ»ΡΡΠ΅Π²ΡΠ΅ ΡΠ»ΠΎΠ²Π°: ΠΊΠ°ΠΏΡΠΎΡΡΠ±ΠΈΠ½, ΠΊΠ°ΠΏΡΠ°Π½ΡΠΈΠ½, Π΄ΠΈΡΡΠΈΡΡ, Lilium pumilum Delile, ΠΎΠ±ΡΠ°ΡΠ΅Π½Π½ΠΎ-ΡΠ°Π·ΠΎΠ²Π°Ρ ΠΠΠΠ₯, APCI, ΡΠ΅Π»Π΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΡΠ°Π·Π΄Π΅Π»Π΅Π½ΠΈΡDOI: http://dx.doi.org/10.15826/analitika.2019.23.4.00
Sorption of 3-glucosides of six common natural anthocyanidins on anatase
In this study, the sorption of 3-glucosides of six common natural aglicones: delphinidin, cyanidin, petunidin, pelargonidin, peonidin and malvidin, on the surface of anatase was studied in comparison with sorption on the surface of silica gel, as a result, a different order of change in the sorption activity of anthocyanins depending on their structure was foun
Determination of carotenoids of tomato fruits of different colors
A method for the determination of the composition of carotenoids of tomato fruits of different colors is proposed using a combination of spectrophotometry and chromatography. An explanation of the elution order of mono-cis-isomers of lycopene under the conditions of reversed-phase chromatography on traditional βmonomericβ reversed C18-phases is proposed for the first time. It was found that, for prolycopene, (7Z, 9Z, 7'Z, 9'Z)-lycopene, which is responsible for the orange color of fruits, there is an almost imperceptible transition in normal examination in the vibronic structure of the electronic absorption spectrum with the lowest energy, Ξ»max(1) = 486.2 n
Selectivity control of dicaffeoylquinic acids separation in reversed-phase hplc with Ξ²-cyclodextrine in a mobile phase
The paper investigates the change in the separation selectivity of the chlorogenic (mono- and dicaffeoylquinic) acids under conditions of reverse-phase HPLC with the addition of Ξ²-cyclodextrin into the mobile phas
Development of a revesed-phase HPLC method for determination of amoxicillin in oral dosage forms
RP HPLC method has been proposed for amoxicillin assay in some drugs. Step bu step development of the chromatographic procedure is considere
Quantum-chemical calculations for the electronic absorption spectra of certain anthocyanidins
Semi-empirical PM3 calculations are made for the electronic absorption spectra of colored forms of nonmethylated anthocyanidins (aurantinidin, pelargonidin, cyanidin, and delphinidin). The position of the absorption band peak is shown to depend on the orientation of hydroxyl groups in the aglycone structur
Antioxidants of Belgorod State University Botanical Garden Plants: Ribes Aureum Fruits Anthocyanins
According to the results of the study, it was found that currant berries of this species contain four main anthocyanins: delfinidin-3-glucoside, delfinidin-3-rutinroside, cyanidin-3-glucoside and cyanidin-3-rutinosid
Non-traditional sources of anthocyanins: flowers of centaurea cyanus (cornflower)
By reverse-phase HPLC with diode-matrix and mass spectrometric detection the species composition of anthocyanins of cornflower petals (Centaureacyanus) of the three colors: blue, red and maroon was determined. For the first time, the anthocyanin composition of cornflower flower extract of dark maroon color, was found to include including the same anthocyanins as cornflower flowers of traditional blue color, but with another rati
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