Rose Flower Petals: Rich Source of Anthocyanins

Dried flower petals of "Tornado" floribunda rose cultivar are a highly abandoned source of anthocyanins with more than 4.0 g of anthocyanins per 100 g of the dryed plant material. The main components of the "Tornado" flower anthocyanin composition were found to be 3,5 -diglucosides of cyanidin (80.9 %) and peonidin (13.9 %

Spectrophotometric determination of oils with radicals of conjugated octadecatrienoic acids

An approach specific for triacylglycerides (TAGs) is proposed for chromatographic separation of compounds with different chromophores using spectrophotometric detection instead of preliminary calibration of the detector using unavailable (or difficultly accessible) reference standard

ПРОБЛЕМЫ ИДЕНТИФИКАЦИИ КСАНТОФИЛЛОВ ЛЕПЕСТКОВ lilium pumilum l ПРИ ПРИМЕНЕНИИ МЕТОДА ОБРАЩЕННО-ФАЗОВОЙ ВЭЖХ СО СПЕКТРОФОТОМЕТРИЧЕСКИМ И МАСС-СПЕКТРОМЕТРИЧЕСКИМ ДЕТЕКТИРОВАНИЕМ

With the use of the reversed-phase high-performance liquid chromatography (HPLC) with diode-array and mass spectrometric detection, the species composition of xanthophylls of dried red color Lilium pumilum Delile flowers was determined. It was established that this plant was a unique source of capsorubin derivatives. The main components of the carotenoid complex were diesters of all-trans and cis-isomers of capsorubin (about 70 mol %) formed by the saturated fatty acids from capric to stearic with the largest contribution of myristic (~ 36 mol %) and lauric (~ 31 mol %) acids. In addition to diethers in the extracts, monoesters of capsorubin and capsanthin were also found (about 30 % of the peak areas). The possibilities of varying the composition of the mobile phase - acetone and propanol-2 as components with the high eluting capacity and acetonitrile and methanol as components with the lower eluting power - for the separation of diethers and differentiation of derivatives of capsanthin and capsorubin and to prevent the imposition of peaks of two series homologues were considered. The features of ion formation during the mass spectrometric detection in the mode of chemical ionization at the atmospheric pressure (APCI) were determined. The possibility of using the parameters of capsorubin diester retention to determine the thermodynamically substantiated dead time of the column was considered.Key words: capsorubin, capsanthin, esters, Lilium pumilum Delile, RP-HPLC, APCI, separation selectivity(Russian) DOI: http://dx.doi.org/10.15826/analitika.2019.23.4.002V.I. Deineka, T.G. Burzhynskaya, L.A. Deineka Belgorod State National Research University, Russian Federation, 308015, Belgorod, ul. Pobedy, 85Предложен способ определения видового состава ксантофиллов высушенных цветков карликовой лилии Lilium pumilum Delile красного цвета с использованием обращенно-фазовой высокоэффективной жидкостной хроматографии с диодно-матричным и масс-спектрометрическим детектированием. Установлено, что данное растение является уникальным растительным источником производных капсорубина. Основными компонентами каротиноидного комплекса являются диэфиры полностью транс- и цис-изомеров капсорубина (около 70 моль %), образованные насыщенными жирными кислотами от каприновой до стеариновой с наибольшим вкладом миристиновой (~ 36 моль %) и лауриновой (~ 31 моль %) кислот. Кроме диэфиров в экстрактах обнаружены моноэфиры капсорубина и капсантина (около 30 % по площадям пиков). Варьирование состава подвижной фазы – ацетона и пропанола-2 как компонентов с высокой элюирующей способностью и ацетонитрила и метанола как компонентов с меньшей элюирующей силой, – позволяет разделять диэфиры и дифференцировать производные капсантина и капсорубина, предотвращая наложения пиков различных гомологов двух рядов. Особенностью масс-спектрометрического детектирования в использованном режиме химической ионизации при атмосферном давлении (APCI) является образование аддуктов с ионами натрия. Показана и обоснована возможность использования параметров удерживания диэфиров капсорубина для определения имеющего термодинамический смысл мертвого времени колонки.Ключевые слова: капсорубин, капсантин, диэфиры, Lilium pumilum Delile, обращенно-фазовая ВЭЖХ, APCI, селективность разделенияDOI: http://dx.doi.org/10.15826/analitika.2019.23.4.00

Sorption of 3-glucosides of six common natural anthocyanidins on anatase

In this study, the sorption of 3-glucosides of six common natural aglicones: delphinidin, cyanidin, petunidin, pelargonidin, peonidin and malvidin, on the surface of anatase was studied in comparison with sorption on the surface of silica gel, as a result, a different order of change in the sorption activity of anthocyanins depending on their structure was foun

Comparison of separation of seed oil triglycerides containing isomeric conjugated octadecatrienoic acid moieties by reversed-phase HPLC

Relative retention analysis and increment approach were applied for the comparison of triglycerides (TGs) retention of a broad set of plant seed oils with isomeric conjugated octadecatrienoic acids (CLnA) by reversed-phase HPLC for "propanol-2-acetonitrile" mobile phases and Kromasil 100-5C18 stationary phase with diode array detection (DAD) and mass spectrometric (MS) detectio

Determination of carotenoids of tomato fruits of different colors

A method for the determination of the composition of carotenoids of tomato fruits of different colors is proposed using a combination of spectrophotometry and chromatography. An explanation of the elution order of mono-cis-isomers of lycopene under the conditions of reversed-phase chromatography on traditional “monomeric” reversed C18-phases is proposed for the first time. It was found that, for prolycopene, (7Z, 9Z, 7'Z, 9'Z)-lycopene, which is responsible for the orange color of fruits, there is an almost imperceptible transition in normal examination in the vibronic structure of the electronic absorption spectrum with the lowest energy, λmax(1) = 486.2 n

Improvement of bactericidal properties of bentonite by means of sodium carbonate

Ecological expediency conditions the necessity to make research of raw materials base and to use for medical purposes those mineral resources which were not subjected to modifications due to aggressive chemical action. Development and sale of pharmaceutical forms with optimal pharmacokinetic characteristics which would not induce occurrence of resistant bacteria population and sensitization of a body is a perspective trend in pharmaceutical engineerin

Selectivity control of dicaffeoylquinic acids separation in reversed-phase hplc with β-cyclodextrine in a mobile phase

The paper investigates the change in the separation selectivity of the chlorogenic (mono- and dicaffeoylquinic) acids under conditions of reverse-phase HPLC with the addition of β-cyclodextrin into the mobile phas

Some aspects of obtaining and use of germinated wheat seeds

Investigation results of the influence of sodium chloride solutions and treatment conditions on wheat seeds germination efficiency are presented in the article. An assortment of products with the addition of germinated seeds, their nutritional and caloric values are presente

Non-traditional sources of anthocyanins: flowers of centaurea cyanus (cornflower)

By reverse-phase HPLC with diode-matrix and mass spectrometric detection the species composition of anthocyanins of cornflower petals (Centaureacyanus) of the three colors: blue, red and maroon was determined. For the first time, the anthocyanin composition of cornflower flower extract of dark maroon color, was found to include including the same anthocyanins as cornflower flowers of traditional blue color, but with another rati