Research on the Phenotypic Characterization of Mrsa Strains Isolated from Animals

Keywords: chromogen, methicillin, MRSA, resistanceIntroduction: Currently, both in staphylococci isolated from animals with different diseases, as well as in humans, the MRSA strains (Methicillin Resistant S. aureus) are monitored, as the methicillin resistance is associated with the resistance to other antibiotic groups.Methicillin resistance is encoded by mec staphylococcal chromosomal cassettes (SCCmec), which are islands of resistance. These strains can be identified by molecular biology tests and tests that reveal several phenotypic characteristics.The research was made in order to characterize and identify phenotypically the MRSA staphylococci strains isolated from animals.Materials and Methods: Researches were made on 240 coagulase positive and coagulase negative strains of staphylococci. Mannitol fermentation was tested on Champan medium, free coagulase was revealed on Baird-Parker medium and to identify S. aureus subsp. aureus was used the chromogenic medium Chromatic Staph.Methicillin-resistant strains were detected by disc diffusion method, using biodiscs with methicillin, oxacillin and cefoxitin. Also, to identify the MRSA strains, was used the chromogenic medium Chromatic MRSA.Results: The isolates were positive to mannitol and produced complete haemolysis or were unhaemolytic. A total of 44 strains produced free coagulase on Baird-Parker medium, considered coagulase positive strains, while 196 were coagulase negative strains.The isolates conducted differently to methicillin: 22,08% of strains were resistant, 51,25% of strains were susceptible and 26,66% had intermediate resistance, while the resistant strains to oxacillin were 42,91%.The increased frequency of methicillin-resistant strains of staphylococci and, particularly, MRSA strains, determined using the cefoxitin disk diffusion test, which is more reliable than methicillin and oxacillin.On the MRSA chromogenic medium, the methicillin-resistant strains of staphylococci formed colonies with pigment from mauve to orange mauve.Conclusion: The obtained results by disk diffusion test on resistance patterns to 3 beta-lactams, resistant to penicillinase, indicated a different frequency of the resistant strains to these antibiotics.Cefoxitin disk diffusion test revealed a frequency of 2,51% of resistant strains, that can be considered MRSA strains

Diagnosticul de laborator al infecţiilor produse de stafilococi la câine

In this review destined for education of specialists in the field are presented methods used in the laboratory diagnosis of various staphylococcal infections. The authors present in detail the methods of sampling, how the samples are transportated, and all laboratory tests, from which, some are done without too much difficulty, even in field conditions, focusing on molecular biology tests. Very important are the two chapters in which are discussed the differentiations between staphylococcus and other bacteria families, but also the antibiotic resistance of the staphilococcuci, a problem of modern medicine current year

Staphylococcus genus - Small practical guide (in Romanian)

In this review destined for education of specialists in the field is presented a bacterial genus important for human and animal health. The authors try to present, in two chapters and in a didactical way, all about Staphylococcus genus, like: taxonomy, morphology, and structure, coloration, culture and cultural characteristics, biochemical properties and antigenic structure, genetics and the antibiotic resistance, ecology and environmental factors, pathogenicity [extracellular enzymes and staphylococcal toxins]. The second chapter is dedicated to the description of natural infections with staphylococci and their implications in humans and animals [large and small ruminants, pigs, equines, rabbits, dogs, cats and birds], the last part of the chapter being devoted to the Staphylococcal food toxicity description

An alternative approach to cat’s epilepsy treatment – Case report

Angel, a female British Shorthair cat, would be euthanized at the recommendation of three veterinarians, after she manifested epileptic seizures starting from seven months old. Due to her owner and one veterinarian love and effort, she well passed through many seizures and a non-regenerative anemia episode and she survived for five years from the onset of the disease. The mineral-vitamin supplements improved the life quality for four years and the homeopathic therapy successfully removed the seizures for one more year