605 research outputs found
Screening Genes for Association with Loci for Nitrogen-Use Efficiency in Perennial Ryegrass by Pyrosequencing\u3csup\u3eTM\u3c/sup\u3e
The application of marker-assisted selection to improve quantitative traits in perennial ryegrass (Lolium perenne) is cumbersome. It requires a priori knowledge on the association of markers and genes. The knowledge on the chromosomal location of major genes for quantitative traits as well as on gene sequences is rapidly growing. However, determination of the genetic constitution of parents prior to their use in breeding still is impractical. More realistic is to collect association data along with the testing activities needed for breeding new varieties. This study uses changes in allele frequency due to selection as a criterion for gene-trait association. Selection-dependent changes are detected with single nucleotide polymorphisms (SNPs) of candidate genes using DNA-pools of F2 plants differing in nitrogen-use efficiency (NUE). The procedure and its feasibility are outlined for one locus
Identification of a Cranberry Juice Product that Inhibits Enteric CYP3A-Mediated First-Pass Metabolism in Humans
An in vivo study in rats showed a cranberry juice product to inhibit the intestinal first-pass metabolism of the CYP3A substrate nifedipine. However, a clinical study involving the CYP3A probe substrate midazolam and a different cranberry juice product showed no interaction. Because the composition of bioactive components in natural products can vary substantially, a systematic in vitro-in vivo approach was taken to identify a cranberry juice capable of inhibiting enteric CYP3A in humans. First, the effects of five cranberry juices, coded A through E, were evaluated on midazolam 1′-hydroxylation activity in human intestinal microsomes. Juice E was the most potent, ablating activity at 0.5% juice (v/v) relative to control. Second, juice E was fractionated to generate hexane-, chloroform-, butanol-, and aqueous-soluble fractions. The hexane- and chloroform-soluble fractions at 50 μg/ml were the most potent, inhibiting by 77 and 63%, respectively, suggesting that the CYP3A inhibitors reside largely in these more lipophilic fractions. Finally, juice E was evaluated on the oral pharmacokinetics of midazolam in 16 healthy volunteers. Relative to water, juice E significantly increased the geometric mean area under the curve (AUC)0-∞ of midazolam by ∼30% (p = 0.001), decreased the geometric mean 1′-hydroxymidazolam/midazolam AUC0-∞ ratio by ∼40% (p < 0.001), and had no effect on geometric mean terminal half-life, indicating inhibition of enteric, but not hepatic, CYP3A-mediated first-pass metabolism of midazolam. This approach both showed a potential drug interaction liability with cranberry juice and substantiated that rigorous in vitro characterization of dietary substances is required before initiation of clinical drug-diet interaction studies
Status of the UCNÏ„ experiment
The neutron is the simplest nuclear system that can be used to probe the structure of the weak interaction and search for physics beyond the standard model. Measurements of neutron lifetime and β-decay correlation coefficients with precisions of 0.02% and 0.1%, respectively, would allow for stringent constraints on new physics. The UCNτ experiment uses an asymmetric magneto-gravitational UCN trap with in situ counting of surviving neutrons to measure the neutron lifetime, τ_n = 877.7s (0.7s)_(stat) (+0.4/−0.2s)_(sys). We discuss the recent result from UCNτ, the status of ongoing data collection and analysis, and the path toward a 0.25 s measurement of the neutron lifetime with UCNτ
Position-sensitive detection of ultracold neutrons with an imaging camera and its implications to spectroscopy
Position-sensitive detection of ultracold neutrons (UCNs) is demonstrated
using an imaging charge-coupled device (CCD) camera. A spatial resolution less
than 15 m has been achieved, which is equivalent to an UCN energy
resolution below 2 pico-electron-volts through the relation . Here, the symbols , , and are the
energy resolution, the spatial resolution, the neutron rest mass and the
gravitational acceleration, respectively. A multilayer surface convertor
described previously is used to capture UCNs and then emits visible light for
CCD imaging. Particle identification and noise rejection are discussed through
the use of light intensity profile analysis. This method allows different types
of UCN spectroscopy and other applications.Comment: 12 figures, 28 pages, accepted for publication in NIM
Measurement of the neutron lifetime using an asymmetric magneto- gravitational trap and in situ detection
The precise value of the mean neutron lifetime, , plays an important
role in nuclear and particle physics and cosmology. It is a key input for
predicting the ratio of protons to helium atoms in the primordial universe and
is used to search for new physics beyond the Standard Model of particle
physics. There is a 3.9 standard deviation discrepancy between
measured by counting the decay rate of free neutrons in a beam (887.7 2.2
s) and by counting surviving ultracold neutrons stored for different storage
times in a material trap (878.50.8 s). The experiment described here
eliminates loss mechanisms present in previous trap experiments by levitating
polarized ultracold neutrons above the surface of an asymmetric storage trap
using a repulsive magnetic field gradient so that the stored neutrons do not
interact with material trap walls and neutrons in quasi-stable orbits rapidly
exit the trap. As a result of this approach and the use of a new in situ
neutron detector, the lifetime reported here (877.7 0.7 (stat) +0.4/-0.2
(sys) s) is the first modern measurement of that does not require
corrections larger than the quoted uncertainties.Comment: 9 pages, 3 figures, 2 table
New result for the neutron -asymmetry parameter from UCNA
The neutron -decay asymmetry parameter defines the correlation
between the spin of the neutron and the momentum of the emitted electron, which
determines , the ratio of the axial-vector to
vector weak coupling constants. The UCNA Experiment, located at the Ultracold
Neutron facility at the Los Alamos Neutron Science Center, is the first to
measure such a correlation coefficient using ultracold neutrons (UCN).
Following improvements to the systematic uncertainties and increased
statistics, we report the new result which yields . Combination with the previous UCNA result and
accounting for correlated systematic uncertainties produces
and .Comment: 9 pages, 7 figures, updated to as-published versio
Application of COMPOCHIP Microarray to Investigate the Bacterial Communities of Different Composts
A microarray spotted with 369 different 16S rRNA gene probes specific to microorganisms involved in the degradation process of organic waste during composting was developed. The microarray was tested with pure cultures, and of the 30,258 individual probe-target hybridization reactions performed, there were only 188 false positive (0.62%) and 22 false negative signals (0.07%). Labeled target DNA was prepared by polymerase chain reaction amplification of 16S rRNA genes using a Cy5-labeled universal bacterial forward primer and a universal reverse primer. The COMPOCHIP microarray was applied to three different compost types (green compost, manure mix compost, and anaerobic digestate compost) of different maturity (2, 8, and 16 weeks), and differences in the microorganisms in the three compost types and maturity stages were observed. Multivariate analysis showed that the bacterial composition of the three composts was different at the beginning of the composting process and became more similar upon maturation. Certain probes (targeting Sphingobacterium, Actinomyces, Xylella/Xanthomonas/ Stenotrophomonas, Microbacterium, Verrucomicrobia, Planctomycetes, Low G + C and Alphaproteobacteria) were more influential in discriminating between different composts. Results from denaturing gradient gel electrophoresis supported those of microarray analysis. This study showed that the COMPOCHIP array is a suitable tool to study bacterial communities in composts
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