Levering proteomic analysis of Pseudomonas fluorescens mediated resistance responses in tomato during pathogenicity of Fusarium oxysporum f. sp. oxysporum

The tomato, one of the world’s most extensively cultivated and consumed vegetable crops is negatively impacted by various pathogens. This study aimed to observe the differentially expressed proteins in tomato samples in plant–pathogen-biocontrol interactions. The fungal pathogen associated with wilted plants were isolated and identified based on its morphological and molecular characteristics. Fourteen strains of Pseudomonas fluorescens from agricultural soils were identified and described using biochemical assays, molecular analyses, and screening for antagonistic ability against the Fusarium wilt pathogen. Results demonstrated that the potential of P. fluorescens (TPf12) positively influenced the expression of antagonism against tomato wilt disease. A total of 14 proteins expressed differently were revealed in the 2D-PAGE-MS investigation. Proteins such as nucleoside diphosphate kinase, phenylalanine ammonia-lyase, protein kinase family protein, Ser/Thr protein kinase-like are unchanged in FOL pathogen interaction, but up-regulated in FOL + TPf12 treated roots, and lipid transfer-like protein, and phenylalanine ammonia-lyase were down-regulated in FOL infested roots and upregulated in FOL + TPf12 treated tomato roots. Phenylalanine ammonia-lyase protein expression is commonly found in TPf12 bioenriched roots, and FOL + TPf12 treated roots, indicating its role in response to the application of TPf12 in tomato. A GC–MS analysis was performed to detect the bioactive metabolites synthesized by TPf12. Molecular docking investigations were conducted using the maestro’s GLIDE docking module of the Schrodinger Software program. Among the secondary metabolites, Cyclohexanepropanoic acid, 2-oxo-, methyl ester (CAS), and 3-o-(4-o-Beta-D-Galactopyranosyl-Beta-D-Galactopyraosyl)-2-Acetylamino-2-Deoxy-D-Galactose were shown to be top-ranked with a least docking score against each differently expressed proteins. The profiled molecules expressed differently due to plant-pathogen-biocontrol interactions may be directly or incidentally involved in the wilt disease resistance of tomato plants

Genetic Diversity Analysis based on the Virulence, Physiology and Regional Variability in Different Isolates of Powdery Mildew in Pea

Powdery mildew is an omnipresent disease that reduces the yield and quality of pea crops (Pisum sativum L.). To examine the powdery mildew pathogen’s morphological, molecular, and genetic diversity, we collected samples of powdery mildew-affected pea crops from ten distinct locations in the Nilgiris district of Tamil Nadu, India. The pathogen Erysiphe pisi was identified morphologically based on anamorphic characters. Molecular identification of E. pisi isolates was befitted by targeting the internal transcribed spacer (ITS) region of rDNA and specific primers of powdery mildew fungi. The genetic variation between ten different E. pisi isolates collected from topographically distinct mountainous areas was studied using random amplified polymorphic (RAPD). Based on its morphological characteristics, the powdery mildew fungus presented high similarities to E. pisi. Molecular characterization of the ITS rDNA of E. pisi produced 650 bp nucleotides, PMITS (powdery mildew-internal transcribed region) primers produced 700 bp nucleotides, and an Erysiphe specific ITS primer pair amplified and synthesized 560 bp nucleotides. According to the findings, the collected E. pisi strains exhibited a low level of genetic diversity and only a slight differential in virulence on the host. In the study, E. pisi isolates from Anumapuram, Emerald Valley, Indira Nagar, and Thuneri showed a greater disease incidence in the natural field conditions and shared the same genetic lineage with other isolates in UPGMA hierarchical cluster analysis based on RAPD markers. There was no evidence of a link between the occurrence of the disease and these grouped populations

Crosstalk of Multi-Omics Platforms with Plants of Therapeutic Importance

From time immemorial, humans have exploited plants as a source of food and medicines. The World Health Organization (WHO) has recorded 21,000 plants with medicinal value out of 300,000 species available worldwide. The promising modern “multi-omics” platforms and tools have been proven as functional platforms able to endow us with comprehensive knowledge of the proteome, genome, transcriptome, and metabolome of medicinal plant systems so as to reveal the novel connected genetic (gene) pathways, proteins, regulator sequences and secondary metabolite (molecule) biosynthetic pathways of various drug and protein molecules from a variety of plants with therapeutic significance. This review paper endeavors to abridge the contemporary advancements in research areas of multi-omics and the information involved in decoding its prospective relevance to the utilization of plants with medicinal value in the present global scenario. The crosstalk of medicinal plants with genomics, transcriptomics, proteomics, and metabolomics approaches will be discussed