A study of correlation of antinuclear antibody immunofluorescence patterns with immune profile using line immunoassay in a tertiary care centre of western Uttar Pradesh

Connective tissue diseases (CTDs) such as systemic lupus erythematosus, systemic sclerosis, Sjogren’s syndrome, and rheumatoid arthritis are systemic diseases which are often associated with a challenge in diagnosis. Antinuclear antibodies (ANA) are autoantibodies that are reactive with antigens in the nucleoplasm. The presence of ANA indicates the possibility of autoimmunity and indirect immunofluorescence (IIF) assay on Hep-2 cells and primate liver is the standard blood test to detect ANA. Our aim of this study is to understand a definite association between ANA patterns and specific antibodies by line immunoassay in the serum of the patients. A total of 360 serum samples from patient attending in the OPD and IPD clinics of the C.S.S.H., Subharti Medical College, Meerut were processed by biochip method. The serum samples which were found positive were confirmed by line immunoassay. In our study 68/360 (18.9%) cases were found to be positive by IIF method in a 1:100 dilution. The positive cases were further confirmed by ANA Profile method. Out of 68 samples positive by ANA-IIF tests, 62 samples gave concordant result with line immunoassay. ANA by IIF method therefore may be used as a cost effective and rapid screening method for patients with criterion for autoimmune diseases in daily clinical practice. These correlations are useful for the diagnosis of a specific rheumatic disease