Additional file 8: of Identification of a neuronal population in the telencephalon essential for fear conditioning in zebrafish

Figure S4. GFP expression patterns in SAGFF120A;UAS:GFP and SAGFF120A;UAS:GFP;UAS:zBoTxBLC:GFP fish. a Dorsal views of the brains from eight SAGFF120A;UAS:GFP (~10 months old) fish and eight SAGFF120A;UAS:GFP;UAS:zBoTxBLC:GFP (~10 months old) fish are shown. Scale bars: 1 mm. b Areas having more intensity than background (the maximum intensity measured in the posterior part of the telencephalon) were identified by using ImageJ [57] and shown in red. Scale bars: 500 Îźm. c Immunohistochemistry using anti-GFP (green) and anti-NeuN (a neuronal marker, magenta) of coronal sections of the telencephalon and hypothalamus of brain samples from these transgenic fish. The fish numbers correspond to the numbers of the dorsal view images. Scale bars, 200 Îźm. (PPTX 6544 kb

Additional file 3: of Identification of a neuronal population in the telencephalon essential for fear conditioning in zebrafish

Figure S2. GFP expression patterns of 16 Gal4FF;UAS:GFP fish that showed reduced performance of the active avoidance response. A dorsal view, a ventral view, and a schematic side view with positions of coronal sections are shown on the top. Serial coronal sections with position numbers are shown in the bottom. a hspGGFF10C, b hspGGFF20A, c hspGFF38B, d hspGFF55B, e SAGFF36B, f SAGFF70A, g SAGFF81B, h SAGFF120A, i SAGFF226F, j SAGFF228A, k SAGFF231A, l SAGFF233A, m SAGFF234A, n SAGFF234D, o hspGFFDMC12A, p hspGFFDMC56B. Scale bars in whole brain images: 500 Îźm. Scale bars in coronal section images: 200 Îźm. (PDF 3264 kb

Additional file 2: of Identification of a neuronal population in the telencephalon essential for fear conditioning in zebrafish

Figure S1. Performance of two-way active avoidance response of double transgenic (Gal4FF;UAS:zBoTxBLC:GFP) fish. The following Gal4FF transgenic fish lines were crossed with UAS:zBoTxBLC:GFP effector fish, and analyzed for two-way active avoidance fear conditioning. a hspGGFF10C (n = 6), b hspGGFF20A (n = 10), c hspGFF38B (n = 10), d hspGFF55B (n = 6), e SAGFF81B (n = 12), f SAGFF226F(n = 9), g SAGFF233A (n = 9), h SAGFF234A (n = 11), i hspGFFDMC12A (n = 12), j hspGFFDMC56B (n = 10), k hspGGFF19B (n = 9), l hspGGFF19C (n = 9), m hspGFF62A (n = 5), n gata6SAGFF94A (n = 6), o SAGFF27C (n = 6), p SAGFF38A (n = 8), q SAGFF87C (n = 5), r SAGFF92A (n = 8), s SAGFF183A (n = 5), t SAGFF195A (n = 6), u SAGFF212C (n = 5), v SAIGFF170B (n = 10), w hspGFFDMC76A (n = 10), x hspGFFDMC85C (n = 11). Mean ± SEM and avoidance (%) for individual fish are plotted. Performance of wild type fish (n = 28) described in Fig. 2 is shown in dotted lines. Two-way ANOVA, fish groups (wild type fish treated by CS-US, wild type fish treated by CS only and double transgenic fish including fish described in Figs. 2 and 3 × training session days (day 1, day 5), was performed (F = 7.236, P < 0.0001). Dunnett’s multiple comparison post-hoc tests were performed between avoidance percentage of wild type fish and double transgenic fish on session day 1 and day 5. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; ns, not significant (P > 0.05). a–j Reduced performance of the active avoidance response was observed. k–x Performance of the active avoidance response was not significantly different between wild type and the double transgenic fish. (PPTX 8767 kb

Additional file 4: of Identification of a neuronal population in the telencephalon essential for fear conditioning in zebrafish

Figure S3. GFP expression patterns in SAGFF120A;UAS:GFP fish at embryonic stages. Bright field and fluorescent images of frontal and lateral views of SAGFF120A;UAS:GFP fish at 24, 48, 72, and 96 hpf. Scale bar, 200 mm. (PPTX 1417 kb