64 research outputs found
Análise comparativa da cicatrização da pele com o uso intraperitoneal de extrato aquoso de Orbignya phalerata (babaçu). Estudo controlado em ratos
The long noncoding RNA HOTAIR has tissue and cell type-dependent effects on HOX gene expression and phenotype of urothelial cancer cells
KINETICS OF B-CELL SUBPOPULATIONS IN PERIPHERAL LYMPHOID-TISSUES - EVIDENCE FOR THE PRESENCE OF PHENOTYPICALLY DISTINCT SHORT-LIVED AND LONG-LIVED B-CELL SUBSETS
A small proportion of the sIg+ B lymphocytes in peripheral lymphoid organs [22% in spleen and 6% in lymph node (LN)] in rat carries the Thy-1 antigen. These Thy-1 + B cells represent newly formed bone marrow (BM) derived (or immature) B cells. In this study we investigated the kinetic behavior of Thy-1+ and Thy-1- B cells in various lymphoid tissues. The renewal rates of these B cells in young adult rats were determined by continuous administration of 5-bromo-2-deoxyuridine (BrdU) for up to 6 weeks. At several time intervals, Thy-1+ and Thy-1- B cell subpopulations in BM, blood, spleen and popliteal LN were analyzed for the presence of incorporated BrdU, using three-color immunocytology on cytospin preparations. In all tissues studied, the Thy-1+ B cells were rapidly renewed with a rate that varied between 58% (BM) and 22% (LN) per day. Virtually all Thy-1+ B cells were labeled by BrdU within a period of 8 - 16 days, indicating that all these cells are relatively short-lived. By contrast, the replacement of Thy-1- B cells in these tissues was 30 - 40 times lower, and ranged between 2.0 and 0.8% per day. In absolute numbers we estimate that 57 million Thy-1+ B cells are renewed per day in the BM whereas only 10 million Thy-1- B cells are replaced in the pool of long-lived peripheral B cells. This implicates a cell loss of 80% at the transition of the Thy-1+ to the Thy-1- B cell stage. The few B cells that actually become incorporated into the pool of mature B cells are most probably selected on the basis of the specificity of their sIg
KINETICS OF B-CELL SUBPOPULATIONS IN PERIPHERAL LYMPHOID-TISSUES - EVIDENCE FOR THE PRESENCE OF PHENOTYPICALLY DISTINCT SHORT-LIVED AND LONG-LIVED B-CELL SUBSETS
A STATHMOKINETIC STUDY OF LYMPHOCYTOPOIESIS-B IN RAT BONE-MARROW - PROLIFERATION OF CELLS CONTAINING CYTOPLASMIC MU-CHAINS, TERMINAL DEOXYNUCLEOTIDYL TRANSFERASE AND CARRYING HIS24 ANTIGEN
A STATHMOKINETIC STUDY OF LYMPHOCYTOPOIESIS-B IN RAT BONE-MARROW - PROLIFERATION OF CELLS CONTAINING CYTOPLASMIC MU-CHAINS, TERMINAL DEOXYNUCLEOTIDYL TRANSFERASE AND CARRYING HIS24 ANTIGEN
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