Seleksi Ketahanan Klon-Klon Harapan Gladiol terhadap Fusarium oxysporum f. sp. gladi oli

Penelitian ini bertujuan untuk mendapatkan klon harapan gladiol yang tahan terhadap layu fusarium.Rancangan yang digunakan adalah acak kelompok pola faktorial. Faktor (1) klon-klon harapan gladiol, terdiri dari96212/168; 96210.2/20; 96215/49; 96203.2/14; 9607.2/129; 96215/202; 96215/122; 96204/69; 96213/109;96210.1/170; hol land merah; dan 621-1. Faktor (2) kerapatan inokulum F. oxysporum, terdiri dari 0 sel konidia/gtanah; 104 sel konidia/g tanah; 108 sel konidia/g tanah. Hasil percobaan menunjukkan bahwa gladiol dengan nomorklon 96215/49; 623-1 dan 96213/109 merupakan klon harapan gladiol yang pal ing tahan terhadap layu F. oxysporumf. sp. dan klon 9612/168 merupakan klon yang pal ing rentan. Re sponse of glad i o lus prom -is ing clones to Fusarium oxysporum f. sp. glad i oli. The aim of the ex per i ment was to ex am ine the re sis tance of glad i -o lus clones to fusarium wilt. Fac to rial randomized block de sign was used in the ex per i ment. The first fac tor wasglad i o lus prom is ing clones, con sist of 96212/168; 96210.2/20; 96215/49; 96203.2/14; 9607.2/129; 96215/202;96215/122; 96204/69; 96213/109; 96210.1/170; hol land merah; 621-1. The sec ond fac tor was den sity of inoculum F.oxysporum, con sist of nill conidia/g soil; 104 cells conidia/g soil; and 108 cells conidia/g soil. The re sults showed thatthe glad i o lus clone num ber 96215/49; 623-1 and 96213/109 were the most re sis tant to Fusarium oxysporum f. sp.glad i oli and clone num ber 9612/168 was the most susceptible

Progeny evaluation from the crossing of novelty-type phalaenopsis I Hsin Bee x Phalaenopsis pulcherrima var. champorensis

The floriculture industry has rapidly developed in Indonesia. One of the potential genera of orchids is Phalaenopsis. New varieties must be developed to compete with imported ones. Genetic improvement of Phalaenopsis has been carried out through interspecific and intergeneric crossing - the crossing between P. I Hsin Bee x P. pulcherrima var. champorensis is dedicated to introduce harlequin type to P. pulcherrima. Forty progenies of the first generation (F1 ) and their parents are used for the characterization and evaluation. The results show that P. pulcherrima is more dominant (50%) in flower shape and colour based on the progeny composition. The introduction of the harlequin type into P. pulcherrima is indicated by 35% of progenies have dark color at the flower and matched with the spotted pattern of the female parent, indicating that the female contributed partially to the respected traits. The progenies are divided into five distinct groups based on nine qualitative characteristics. Three progenies, namely D829-4, D829-6, and D829-8, are considered to have prominent features, such as more stable and symmetric coalescence of red-brownish blotches on the flowers, representing harlequin type than other progenies. These progenies will potentially be developed to be commercial varieties

Consumer Preference Analysis of Phalaenopsis Orchid Variety

The majority of new Phalaenopsis orchid varieties circulating in Indonesia are still imported from abroad. To limit imports, orchid breeders was trying to produce new crossbred varieties that were needed by orchid producers and lovers in Indonesia. Before being distributed to orchid lovers, a consumer preference test was conducted to find out which orchid varieties were favored by consumers. In this regard, the aim of the study was to assess consumer preferences of Phalaenopsis orchid variety. The survey was carried out online on June 20-27, 2022, among 215 respondents from DKI Jakarta and West Java, with questionnaires distributed personally via social media links (WhatsApp). Respondent selection was done by simple randomization (simple random sampling). The Chi Square test was used to analyze the data. The results showed that there is sufficient evidence to reject H0 and accept H1. Candidate varieties of Phalaenopsis 1 (Puspita Devi Agrihorti) are preferred over other varieties. There is a considerable variation in the distribution of preferences between the candidate variety and the comparison variety of Phalaenopsis. The implication of this research was that the existence of new varieties of orchids can reduce our dependence on Phalaenopsis orchid varieties from abroad

<i>In vitro</i> Embryogenesis Derived from Shoot Tips in Mass Propagation of Two Selected-Clones of <i>Phalaenopsis</i>

Phalaenopsis is of high economic value and market demand in Indonesia; however, orchid products are mostly imported from other countries. ‘Kristina Dwi’ (KD) 69.274 and ‘Dedeh’ (D) 802.28 are two selected clones with high potential utilized and developed commercially. To support their commercialization, a reliable in vitro propagation protocol is essential.  In the current study, an in vitro mass propagation protocol for KD 69.274 and D 802.28 clones was successfully established using shoot tips as explant sources. A high number of embryos, up to 8.2 embryos per explant, with 58.5% explant regeneration, and 3.5 regenerated-explants in average were regenerated from shoot tips of KD 69.274 clone cultured on  half-strength Murashige and Skoog (MS) medium, with full strength micro, Fe-chellate and vitamin containing 0.5 mg/L thidiazuruon (TDZ) and 0.25 mg/L N6-benzyladenine (BA). The initial embryos were proliferated by culturing embryos individually on half-strength MS medium with 0.13 mg/L TDZ and 0.25 mg/L BA and resulted in high embryo regeneration up to 91.4%, with 10.2 embryos per explant and no embryo browning. The embryos were multiplied under periodical subcultures of 3 months each, resulting in gradual increasing number of embryos from the first subculture till the fifth subculture, with 23.6 embryos produced, then declined afterward. The embryos were easily germinated on half-strength MS medium with full strength of vitamin and hormone free, with 73.9% embryo germination and 14.9 germinated embryos. Healthy plantlets were stimulated on the same medium with 2 g/L activated charcoal (AC) and successfully acclimatized on Cycas rumphii bulk, with 88.3% survival plantlets. Finally, it can be summarized that a new in vitro mass propagation protocol, as new alternative choice for Phalaenopsis propagation, was successfully established

Supplementary Data of PCTO-S-24-00118-3 manuscript

Our manuscript investigates the complexities of genotype interactions, quantitative and qualitative characters, and variables pertinent to in vitro culture initiation abilities in Vanda orchids. Through a meticulous study involving 56 hybrids and four registered varieties resulting from a breeding program in Indonesia, we identified distinctive genotype clusters and their correlations with various floral traits and explant performance metrics. Additionally, we delved into optimizing media compositions, explant types, and culture conditions to enhance proliferation rates and overall propagation success.</p

Supplementary Tables of PCTO-S-24-00118-3 manuscript

Our manuscript investigates the complexities of genotype interactions, quantitative and qualitative characters, and variables pertinent to in vitro culture initiation abilities in Vanda orchids. Through a meticulous study involving 56 hybrids and four registered varieties resulting from a breeding program in Indonesia, we identified distinctive genotype clusters and their correlations with various floral traits and explant performance metrics. Additionally, we delved into optimizing media compositions, explant types, and culture conditions to enhance proliferation rates and overall propagation success.</p