SECOND INTERNATIONAL SYMPOSIUM ON RANAVIRUSES:: A NORTH AMERICAN HERPETOLOGICAL PERSPECTIVE

Ranaviruses are large double stranded DNA viruses of poikilothermic vertebrates including amphibians, reptiles and fish. In North America, ranaviral disease and ranavirus-related die-off events have been documented in all three classes. Ranaviruses are found worldwide, appear to be emerging in some regions, and are increasingly recognized as a threat to many species

Disease monitoring and biosecurity

Understanding and detecting diseases of amphibians has become vitally important in conservation and ecological studies in the twenty-fi rst century. Disease is defi ned as the deviance from normal conditions in an organism. The etiologies (causes) of disease include infectious, toxic, traumatic, metabolic, and neoplastic agents. Thus, monitoring disease in nature can be complex. For amphibians, infectious, parasitic, and toxic etiologies have gained the most notoriety. Amphibian diseases have been linked to declining amphibian populations, are a constant threat to endangered species, and are frequently a hazard in captive breeding programs, translocations, and repatriations. For example, a group of viruses belonging to the genus Ranavirus and the fungus Batrachochytrium dendrobatidis are amphibian pathogens that are globally distributed and responsible for catastrophic population die-offs, with B. dendrobatidis causing known species extinctions (Daszak et al. 1999; Lips et al. 2006; Skerratt et al. 2007). Some infectious diseases of amphibians share similar pathological changes; thus, their detection, recognition, and correct diagnosis can be a challenge even by trained veterinary pathologists or experienced herpetologists. This chapter will introduce readers to the most common amphibian diseases with an emphasis on those that are potentially or frequently lethal, and the techniques involved in disease monitoring. It will also outline methods of biosecurity to reduce the transmission of disease agents by humans. We start by covering infectious, parasitic, and toxic diseases. Next, surveillance methods are discussed, including methods for sample collection and techniques used in disease diagnosis. Finally, biosecurity issues for preventing disease transmission will be covered, and we provide protocols for disinfecting fi eld equipment and footwear

Frequency and Use of Medications in Horses Racing at Prairie Meadows

An analysis was made of the horses racing at Prairie Meadows race track in Altoona, Iowa during 1993 to determine the number of entries designated as racing under the influence of phenylbutazone (Bute(RX)), furosemide (Lasix(Rx)) or both medications. In a total of 1379 Quarter Horse entries, 5.7 % raced with no medication, 74.9 % raced on phenylbutazone, 0.5 % raced on furosemide, and 18.9 % raced on both phenylbutazone and furosemide. In a total of 3424 Thoroughbred entries, 2.1 % raced under no medication, 43.6 % raced on phenylbutazone, 0.4 % raced on furosemide, and 53.9 % raced on both phenylbutazone and furosemide. Overall, of the 4803 entries, 3.2 % raced with no medication, 52.6 % raced on phenylbutazone, 0.4 % raced on furosemide, and 43.9 % raced on both phenylbutazone and furosemide

The Effect of Furosemide on Arterial Blood Gases and Performance in Quarter Horses Performing a Fatigue Test on a Treadmill

Four Quarter Horses (1 filly age 2, 1 mare age 5 and 2 geldings ages 3 and 4; average weight 539 kg) were used in a 2 x 2 crossover design. The effects of furosemide (Lasix(Rx)) on arterial blood packed ceii voiume (PCV), hemogiobin (Hb), pH, pO2, pCO2, HCO-3 and base excess (BE) were measured. Plasma lactate, heart rate, and fatigue time were determined as indicators of perlormance while the horses performed a fatigue test on a high-speed treadmill. The left carotid artery was surgically elevated subcutaneously to facilitate collection of arterial blood samples. Horses were conditioned for 13 weeks with increasing intensity then randomly assigned furosemide (F) or physiological saline (C) as treatments. Treatments were administered 4 hours prior to the fatigue test in accordance with racing regulations. Arterial blood samples were collected prior to treatment dose, prior to exercise, at the 2nd, 4th, and 6th minute during the fatigue test, at fatigue, and at the 5th, 15th, 30th, and 45th minute post-exercise. Arterial blood samples were analyzed for blood gases, Hb, PCV, and plasma lactate. Heart rate and fatigue time were recorded. No difference between treatments (P \u3e 0.05) was observed for blood gases except for pCO2 at rest, and HCO-3 and BE at the 2 minute collection period. No difference between treatments (P \u3e 0.05) was observed for Hb, PCV, lactate and heart rate except at 15 minutes post-exercise for Hb and PCV, and 45 minutes postexercise for Hb. Fatigue times were 11 min 56 sec ± 5 min 30 sec for F horses and 11 min 35 sec--± 2 min 6 sec for C horses. No difference (P \u3e 0.05) was observed in fatigue time. Based on our data, the trend indicated that all parameters measured returned to pre-exercise levels more rapidly for furosemide treated horses. However, furosemide did not enhance performance

Development and initial testing of the self‐care of chronic illness inventory

Aim The aim was to develop and psychometrically test the self‐care of chronic illness Inventory, a generic measure of self‐care. Background Existing measures of self‐care are disease‐specific or behaviour‐specific; no generic measure of self‐care exists. Design Cross‐sectional survey. Methods We developed a 20‐item self‐report instrument based on the Middle Range Theory of Self‐Care of Chronic Illness, with three separate scales measuring Self‐Care Maintenance, Self‐Care Monitoring, and Self‐Care Management. Each of the three scales is scored separately and standardized 0–100 with higher scores indicating better self‐care. After demonstrating content validity, psychometric testing was conducted in a convenience sample of 407 adults (enrolled from inpatient and outpatient settings at five sites in the United States and ResearchMatch.org). Dimensionality testing with confirmatory factor analysis preceded reliability testing. Results The Self‐Care Maintenance scale (eight items, two dimensions: illness‐related and health‐promoting behaviour) fit well when tested with a two‐factor confirmatory model. The Self‐Care Monitoring scale (five items, single factor) fitted well. The Self‐Care Management scale (seven items, two factors: autonomous and consulting behaviour), when tested with a two‐factor confirmatory model, fitted adequately. A simultaneous confirmatory factor analysis on the combined set of items supported the more general model. Conclusion The self‐care of chronic illness inventory is adequate in reliability and validity. We suggest further testing in diverse populations of patients with chronic illnesses

Fusarium spp. an emerging fungal threat to leatherback (Dermochelys coriacea) eggs and neonates

IntroductionFungal diseases are a rising health problem globally, in humans, nonhuman animals, and plants. Emerging fungal diseases have been associated with mass mortality events. A recent example of fungal disease pathogenicity is sea turtle egg fusariosis (STEF). The pathogenicity of STEF has been linked to fungi within the Fusarium solani species complex (FSSC). This complex is composed of over 45 phylogenetically identifiable species commonly found in the environment. Species within the FSSC lineage have been isolated from the nests of multiple sea turtle species and are linked to decreased hatching success in all 7 of the extant sea turtle species. Fungi within this lineage are also known to cause cutaneous and subcutaneous infections. These fungi are not only a threat to sea turtles but also to other animals, including humans, that use coastal waters and beaches inhabited by Fusarium spp. The presence of Fusarium spp., in the context of sea turtle health, has not been investigated on southeastern Florida beaches which are fundamentally important for at least three sea turtle species that nest there in large numbers.MethodsWe performed a retrospective assessment of necropsy reports from dead captive leatherback neonates from 2010 to 2022 to assess the most common microscopic diagnoses and the presence of skin lesions associated with mycotic dermatitis. Additionally, live captive leatherbacks and dead-in-nest samples from the 2022 hatching season were used to assess the presence and effect of mycotic dermatitis in Juno Beach and Boca Raton, Florida, USA. This was accomplished by observing gross lesions, fungal cultures, and blood values.ResultsThe retrospective analysis of dead captive neonates revealed that the diagnosis of mycotic dermatitis on histopathology has significantly increased since 2010. The assessment of gross skin lesions associated with mycotic dermatitis in dead and live captive leatherback neonates also revealed a similar increase. Investigations in live captive leatherbacks revealed fungal cultures positive for Fusarium spp. and significant differences in blood values at emergence between healthy turtles and those that later developed mycotic dermatitis.DiscussionPositive dead-in-nest culture results suggest that Fusarium spp. are likely present in leatherback sea turtle nests in Boca Raton and Juno Beach, Florida, USA. Additionally, the occurrence of mycotic dermatitis in dead and live captive leatherback neonates suggests that the presence of Fusarium spp. in the nest likely affects leatherback neonates even after emergence

Intrahistiocytic Storage of Clofazimine Crystals in a Cat

A 13-year-old castrated male Maine coon cat with a 5-year history of atypical mycobacteriosis was euthanized and submitted for necropsy. The cat had been kept in clinical remission since diagnosis using a combination of the antimycobacterial drug clofazimine and additional multimodal antimicrobial therapy. Grossly, tissues were diffusely discolored red-brown to yellow. Histologically, the myocardial interstitum was expanded by numerous, often multinucleated cells, which were distended by uniformly shaped acicular cytoplasmic spaces. These cells were immunopositive for CD18 and immunonegative for desmin, suggesting a histiocytic rather than muscular origin. Macrophages in other tissues contained similar acicular spaces. Ultrastructurally, the spaces were surrounded by 2 lipid membranes, resembling an autophagosome. Based on the clinical history and histologic, immunohistochemical, and ultrastructural data, we diagnosed clofazimine crystal storage. To our knowledge, this is the first report of clofazimine storage in a cat or within myocardial interstitial macrophages

Prolonged Mitosis of Neural Progenitors Alters Cell Fate in the Developing Brain

Embryonic neocortical development depends on balanced production of progenitors and neurons. Genetic mutations disrupting progenitor mitosis frequently impair neurogenesis; however, the link between altered mitosis and cell fate remains poorly understood. Here we demonstrate that prolonged mitosis of radial glial progenitors directly alters neuronal fate specification and progeny viability. Live imaging of progenitors from a neurogenesis mutant, Magoh(+/-), reveals that mitotic delay significantly correlates with preferential production of neurons instead of progenitors, as well as apoptotic progeny. Independently, two pharmacological approaches reveal a causal relationship between mitotic delay and progeny fate. As mitotic duration increases, progenitors produce substantially more apoptotic progeny or neurons. We show that apoptosis, but not differentiation, is p53 dependent, demonstrating that these are distinct outcomes of mitotic delay. Together our findings reveal that prolonged mitosis is sufficient to alter fates of radial glia progeny and define a new paradigm to understand how mitosis perturbations underlie brain size disorders such as microcephaly