Effect of storage duration and analyte concentration on measurement of neutral pH clinical chemistry urine analytes

Background: The aim of the study was focused very keenly in measuring the changes in concentration of sodium, potassium and creatinine in urine, stored for 60 days effect of urinary storage duration of clinical chemistry analytes. This study determined whether patient urine samples can be used for day to day urine quality control.Methods: Spot urine samples were collected from left over samples from the admitted patients in various hospital wards. The samples are kept which its pH was unaltered or unspiked and was aliquoted and deep fridged immediately. These aliquots were melted at an incubator temperature of 86 ºC and was analyzed in the analyzer for 60 days and data were collected for checking variations.Results: With the strenous and continious monitoring from the side of the researchers, the researchers had throughly analysed and found that in both the sets almost all of those analysed urine test of pH 5 and analytes remained very much stable for a period of 60 days which could be used for running daily internal quality controls.Conclusions: Patient urine can be used as internal quality control sample for at least 60 days for sodium, potassium and creatinine. During the 60 days period, there is no appreciable degradation of any of the two sets of samples for sodium, potassium and creatinine measurements

Comparison of direct total iron binding capacity by light MgCO3 and heavy MgCO3

Background: The aim of the study is focused to measure TIBC by calculated method (TIBC using the magnesium carbonate- MgCO3 adsorption), measurement of TIBC by calculatory method from automated UIBC and automated Iron measurement. Latter these two values are to be added and would give an additive result of TIBC. The experiment would be performed by using the light MgCO3 powder and the heavy MgCO3 powder and check for any differences in their respective values. Regression analysis, Bland Altman analysis or histogram analysis of TIBC results obtained by calculated methods in patient samples to find correlation between the two methods is being performed.Methods: Samples received for analysis of tests, regardless of patient identification with sample volume serum were used for the study. After complete analysis and reporting of the sample, the leftover serum was used. Serum was kept at room temperature. Then the calculated TIBC from UIBC and the IRON were compared after doing batch calibration for UIBC and lot calibration of Iron.Results: It was found that, this study had positive bias by the usage of the heavy MgCO3 powder. Positive bias in unsaturated iron binding capacity observed by both methods is studied and further investigated using data obtained during the experiment.Conclusions: There is a higher yield of serum obtained with the heavy MgCO3 powder, TIBC calculated from iron and unsaturated iron binding capacity as compared to TIBC measured directly using the light MgCO3 powder

EDTA contamination: a preanalytical cause for interference in iron and unsaturated iron binding capacity assay

Background: The aim of the study is focussed very keenly at performing RCA (root cause analysis) of those particular sample containing the absurd results of the Serum UIBC (Unsaturated Iron Binding Capacity), which was also tallied vigilantly side by side with Serum Iron observed at Clinical Biochemistry laboratory of The New Civil Hospital Surat, Gujarat.Methods: The Absurd value from the samples requested for Serum Iiron and Serum Serum UIBC (Unsaturated Iron Binding Capacity) from month of August 2018 till the month of August 2019 were taken in to account for analysis. RCA (root cause analysis) of absurd value for Serum Iron and Serum UIBC (Unsaturated Iron Binding Capacity) which were prepared was mainly focused on tallying with Serum Calcium and Serum Potassium result.Results: With the continious and strenous monitoring from the side of the researchers, the researchers had throughly analysed and found that in almost all of those analysed test containing absurd results of Serum Iron and Serum UIBC(Unsaturated Iron Binding Capacity) which were some way or the other, associated with absurd results of Serum Calcium along with absurd result of Serum Potassium too from the same samples.Conclusions: The Absurd results of Serum Calcium and Serum Potassium are caused mainly due to pre-analytical errors more likely due to the sample contamination with EDTA (ethylene diamine tetra acetic acid). The contaminated EDTA (ethylene diamine tetra acetic acid) sample cause interference in Serum Iron measurement by producing turbidity in sample and in Serum UIBC (Unsaturated Iron Binding Capacity) by chelating Iron