3 research outputs found
Identification and Engineering of Aptamers for Theranostic Application in Human Health and Disorders
An aptamer is a short sequence of synthetic oligonucleotides which bind to their cognate target, specifically while maintaining similar or higher sensitivity compared to an antibody. The in-vitro selection of an aptamer, applying a conjoining approach of chemistry and molecular biology, is referred as Systematic Evolution of Ligands by Exponential enrichment (SELEX). These initial products of SELEX are further modified chemically in an attempt to make them stable in biofluid, avoiding nuclease digestion and renal clearance. While the modification is incorporated, enough care should be taken to maintain its sensitivity and specificity. These modifications and several improvisations have widened the window frame of aptamer applications that are currently not only restricted to in-vitro systems, but have also been used in molecular imaging for disease pathology and treatment. In the food industry, it has been used as sensor for detection of different diseases and fungal infections. In this review, we have discussed a brief history of its journey, along with applications where its role as a therapeutic plus diagnostic (theranostic) tool has been demonstrated. We have also highlighted the potential aptamer-mediated strategies for molecular targeting of COVID-19. Finally, the review focused on its future prospective in immunotherapy, as well as in identification of novel biomarkers in stem cells and also in single cell proteomics (scProteomics) to study intra or inter-tumor heterogeneity at the protein level. Small size, chemical synthesis, low batch variation, cost effectiveness, long shelf life and low immunogenicity provide advantages to the aptamer over the antibody. These physical and chemical properties of aptamers render them as a strong biomedical tool for theranostic purposes over the existing ones. The significance of aptamers in human health was the key finding of this review
Involvement of novel PKC isoforms in FFA induced defects in insulin signaling
Involvement of novel PKCs (nPKCs) in the negative regulation of insulin-signaling pathway is a current interest of many workers investigating the cause for insulin resistance and type 2 diabetes. Free fatty acids (FFAs) are recently shown to be the major players in inducing insulin resistance in insulin target cells. They are also found to be involved in activating nPKCs associated with the impairment of insulin sensitivity. In this overview, we describe PKC δ, θ and ε linked to the FFA induced damage of insulin-signaling molecules
Inhibition of Insulin Receptor Gene Expression and Insulin Signaling by Fatty Acid: Interplay of PKC Isoforms Therein
Fatty acids are known to play a key role in promoting
the loss of insulin sensitivity causing insulin resistance
and type 2 diabetes. However, underlying mechanism
involved here is still unclear. Incubation of rat skeletal
muscle cells with palmitate followed by I125- insulin
binding to the plasma membrane receptor preparation
demonstrated a two-fold decrease in receptor
occupation. In searching the cause for this reduction,
we found that palmitate inhibition of insulin receptor
(IR) gene expression effecting reduced amount of IR
protein in skeletal muscle cells. This was followed by
the inhibition of insulin-stimulated IRβ tyrosine
phosphorylation that consequently resulted inhibition
of insulin receptor substrate 1 (IRS 1) and IRS 1
associated phosphatidylinositol-3 kinase (PI3 Kinase),
phosphoinositide dependent kinase-1 (PDK 1)
phosphorylation. PDK 1 dependent phosphorylation
of PKCζ and Akt/PKB were also inhibited by palmitate.
Surprisingly, although PKCε phosphorylation is PDK1 dependent, palmitate effected its constitutive
phosphorylation independent of PDK1. Time kinetics
study showed translocation of palmitate induced
phosphorylated PKCε from cell membrane to nuclear
region and its possible association with the inhibition
of IR gene transcription. Our study suggests one of
the pathways through which fatty acid can induce
insulin resistance in skeletal muscle cell