84 research outputs found
Usefulness of pulse oximetry using the SET technology in critically ill adult patients
International audienc
What Glues a Homodimer Together Systematic Analysis of the Stabilizing Effect of an Aromatic Hot Spot in the Protein Protein Interface of the tRNA Modifying Enzyme Tgt
Shigella bacteria constitute the causative agent of bacillary dysentery, an acute inflammatory disease causing the death of more than one million humans per year. A null mutation in the tgt gene encoding the tRNA modifying enzyme tRNA guanine transglycosylase Tgt was found to drastically decrease the pathogenicity of Shigella bacteria, suggesting the use of Tgt as putative target for selective antibiotics. The enzyme is only functionally active as a homodimer; thus, interference with the formation of its protein protein interface is an attractive opportunity for therapeutic intervention. To better understand the driving forces responsible for the assembly, stability, and formation of the homodimer, we studied the properties of the residues that establish the dimer interface in detail. We performed site directed mutagenesis and controlled shifts in the monomer dimer equilibrium ratio in solution in a concentration dependent manner by native mass spectrometry and used crystal structure analysis to elucidate the geometrical modulations resulting from mutational variations. The wild type enzyme exhibits nearly exclusive dimer geometry. A patch of four aromatic amino acids, embedded into a ring of hydrophobic residues and further stabilized by a network of H bonds, is essential for the stability of the dimer s contact. Accordingly, any perturbance in the constitution of this aromatic patch by nonaromatic residues reduces dimer stability significantly, with some of these exchanges resulting in a nearly exclusively monomeric state. Apart from the aromatic hot spot, the interface comprises an extended loop helix motif that exhibits remarkable flexibility. In the destabilized mutated variants, the loop helix motif adopts deviating conformations in the interface region, and a number of water molecules, penetrating into the interface, are observe
Launching Spiking Ligands into a Protein Protein Interface A Promising Strategy To Destabilize and Break Interface Formation in a tRNA Modifying Enzyme
Hot spot analysis to dissect the functional protein protein interface of a tRNA modifying enzyme
Reproductibilité de la mesure échographique de la surface antrale gastrique chez le volontaire sain
Medical students trained in communication skills show a decline in patient-centred attitudes : an observational study comparing two cohorts during clinical clerkships
Use of virtual screening for discovering antiretroviral compounds interacting with the HIV-1 nucleocapsid protein.
The HIV-1 nucleocapsid protein (NC) is considered as an emerging drug target for the therapy of AIDS. Several studies have highlighted the crucial role of NC within the viral replication cycle. However, although NC inhibition has provided in vitro and in vivo antiretroviral activity, drug-candidates which interfere with NC functions are still missing in the therapeutic arsenal against HIV. Based on previous studies, where the dynamic behavior of NC and its ligand binding properties have been investigated by means of computational methods, here we used a virtual screening protocol for discovering novel antiretroviral compounds which interact with NC. The antiretroviral activity of virtual hits was tested in vitro, whereas biophysical studies elucidated the direct interaction of most active compounds with NC(11-55), a peptide corresponding to the zinc finger domain of NC. Two novel antiretroviral small molecules capable of interacting with NC are presented here
Please don't touch me there - How medical students are being taught at University of Antwerp
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