Differences in Calculations of Annual Heating and Cooling Energy Need Carried out by Modified Simple Hourly Method and Dynamic Simulations

This paper presents differences in calculations of annual heating and cooling energy need performed by numerical dynamic simulations software IDA ICE and those carried out by the modification of simple hourly method from EN ISO 13790 EN ISO 13790, widely used for determining building energy need. A simple model of a nearly-zero energy building was created and all heat gains and set-points that could lead to a mismatch in initial or boundary conditions were analysed. The impact of those on the annual heating and cooling energy need was examined by adding and/or removing every single one of them. Boundary conditions in numerical dynamic simulations were set up to match those in simple hourly method. Such an approach enables evaluation of differences in results and definition of their origin. The comparison of results has shown that in most cases, annual energy need for heating and cooling calculated using numerical dynamic simulations software differs from that calculated using EN ISO 13790. Among the others, more detailed heat accumulation model of heat gains in building’s envelope in IDA ICE software was marked as the main reason. Fact that solar heat gains seem to be underestimated by EN ISO 13790 and differences in heat transfer towards ground contribute to the differences in results as well

A hub and spoke nuclear lamina architecture in trypanosomes

The nuclear lamina supports many functions, including maintaining nuclear structure and gene expression control, and correct spatio-temporal assembly is vital to meet these activities. Recently, multiple lamina systems have been described that, despite independent evolutionary origins, share analogous functions. In trypanosomatids the two known lamina proteins, NUP-1 and NUP-2, have molecular masses of 450 and 170 kDa, respectively, which demands a distinct architecture from the ∼60 kDa lamin-based system of metazoa and other lineages. To uncover organizational principles for the trypanosome lamina we generated NUP-1 deletion mutants to identify domains and their arrangements responsible for oligomerization. We found that both the N- and C-termini act as interaction hubs, and that perturbation of these interactions impacts additional components of the lamina and nuclear envelope. Furthermore, the assembly of NUP-1 terminal domains suggests intrinsic organizational capacity. Remarkably, there is little impact on silencing of telomeric variant surface glycoprotein genes. We suggest that both terminal domains of NUP-1 have roles in assembling the trypanosome lamina and propose a novel architecture based on a hub-and-spoke configuration