14,361 research outputs found
Serotonin drives a novel GABAergic synaptic current recorded in rat cerebellar purkinje cells: a lugaro cell to purkinje cell synapse
We recorded a novel fast GABAergic synaptic current in cerebellar Purkinje cells in rat brain slices using patch-clamp techniques. Because of a relatively low sensitivity to bicuculline, these currents can be recorded under conditions in which basket and stellate cell inputs are blocked. The observations that the novel synaptic currents occur spontaneously only in the presence of serotonin, and the specific limited positions in the slice from which they can be electrically evoked, suggest that the presynaptic cell is the Lugaro cell. Cell-attached recordings confirm that the Lugaro cell is the only interneuron in the cerebellar cortex with firing behavior consistent with the spontaneous activity recorded in Purkinje cells. The input shows a strong presynaptic modulation mediated by GABAA receptors, resulting in a dynamic range from almost 0 to >90% release probability. Modeling GABAA receptor responses to different GABA transients suggests that the relatively low sensitivity of the synaptic currents to bicuculline, compared with the higher affinity GABAA receptor antagonist SR-95531 (2-(3-carboxypropyl)-3-amino-6-(4-methoxyphenyl) pyridazinium), is attributable to an unusually long GABA dwell time and/or high GABA concentration in the synaptic cleft. The significance of this novel input is discussed in relation to other GABAergic synapses impinging on Purkinje cells. We suggest that the release of GABA onto Purkinje cells from Lugaro cells would primarily occur during motor activity under conditions in which the activity of basket and stellate cells might be inhibited
Serotonin drives a novel GABAergic synaptic current recorded in rat cerebellar purkinje cells: a lugaro cell to Purkinje cell synapse
We recorded a novel fast GABAergic synaptic current in cerebellar Purkinje cells in rat brain slices using patch-clamp techniques. Because of a relatively low sensitivity to bicuculline, these currents can be recorded under conditions in which basket and stellate cell inputs are blocked. The observations that the novel synaptic currents occur spontaneously only in the presence of serotonin, and the specific limited positions in the slice from which they can be electrically evoked, suggest that the presynaptic cell is the Lugaro cell. Cell-attached recordings confirm that the Lugaro cell is the only interneuron in the cerebellar cortex with firing behavior consistent with the spontaneous activity recorded in Purkinje cells. The input shows a strong presynaptic modulation mediated by GABAA receptors, resulting in a dynamic range from almost 0 to >90% release probability. Modeling GABAA receptor responses to different GABA transients suggests that the relatively low sensitivity of the synaptic currents to bicuculline, compared with the higher affinity GABAA receptor antagonist SR-95531 (2-(3-carboxypropyl)-3-amino-6-(4-methoxyphenyl) pyridazinium), is attributable to an unusually long GABA dwell time and/or high GABA concentration in the synaptic cleft. The significance of this novel input is discussed in relation to other GABAergic synapses impinging on Purkinje cells. We suggest that the release of GABA onto Purkinje cells from Lugaro cells would primarily occur during motor activity under conditions in which the activity of basket and stellate cells might be inhibited
Effective diffusion constant in a two dimensional medium of charged point scatterers
We obtain exact results for the effective diffusion constant of a two
dimensional Langevin tracer particle in the force field generated by charged
point scatterers with quenched positions. We show that if the point scatterers
have a screened Coulomb (Yukawa) potential and are uniformly and independently
distributed then the effective diffusion constant obeys the
Volgel-Fulcher-Tammann law where it vanishes. Exact results are also obtained
for pure Coulomb scatterers frozen in an equilibrium configuration of the same
temperature as that of the tracer.Comment: 9 pages IOP LaTex, no figure
The Steady-State Growth Theorem: A Comment on Uzawa (1961)
This brief note revisits the proof of the Steady-State Growth Theorem, first provided by Uzawa (1961). We provide a clear statement of the theorem and a new version of Uzawa's proof that makes the intuition underlying the result more apparent.
Dynamical transition for a particle in a squared Gaussian potential
We study the problem of a Brownian particle diffusing in finite dimensions in
a potential given by where is Gaussian random field.
Exact results for the diffusion constant in the high temperature phase are
given in one and two dimensions and it is shown to vanish in a power-law
fashion at the dynamical transition temperature. Our results are confronted
with numerical simulations where the Gaussian field is constructed, in a
standard way, as a sum over random Fourier modes. We show that when the number
of Fourier modes is finite the low temperature diffusion constant becomes
non-zero and has an Arrhenius form. Thus we have a simple model with a fully
understood finite size scaling theory for the dynamical transition. In addition
we analyse the nature of the anomalous diffusion in the low temperature regime
and show that the anomalous exponent agrees with that predicted by a trap
model.Comment: 18 pages, 4 figures .eps, JPA styl
Renormalization of Drift and Diffusivity in Random Gradient Flows
We investigate the relationship between the effective diffusivity and
effective drift of a particle moving in a random medium. The velocity of the
particle combines a white noise diffusion process with a local drift term that
depends linearly on the gradient of a gaussian random field with homogeneous
statistics. The theoretical analysis is confirmed by numerical simulation. For
the purely isotropic case the simulation, which measures the effective drift
directly in a constant gradient background field, confirms the result
previously obtained theoretically, that the effective diffusivity and effective
drift are renormalized by the same factor from their local values. For this
isotropic case we provide an intuitive explanation, based on a {\it spatial}
average of local drift, for the renormalization of the effective drift
parameter relative to its local value. We also investigate situations in which
the isotropy is broken by the tensorial relationship of the local drift to the
gradient of the random field. We find that the numerical simulation confirms a
relatively simple renormalization group calculation for the effective
diffusivity and drift tensors.Comment: Latex 16 pages, 5 figures ep
Crystallization and preliminary X-ray analysis of a D-alanyl-D-alanine ligase (EcDdlB) from Escherichia coli
A recombinant form of Escherichia coli DdlB (EcDdlB) has been prepared and cocrystallized with ADP and D-alanyl-D-alanine to represent the ternary complex of EcDdlB. Furthermore, EcDdlB has been cocrystallized under the same conditions with the ligands ATP and D-alanyl-D-alanine, representing the product-inhibited complex. The crystals belonged to space group P212121, with unit-cell parameters a = 53.0, b = 97.6, c = 109.5 Ã… and a = 51.2, b = 97.8, c = 110.1 Ã…, respectively, and both contained two molecules in the asymmetric unit. Complete data sets were collected to 1.5 and 1.4 Ã… resolution, respectively, from single crystals under cryogenic conditions using synchrotron radiation
Distributed Deep Learning for Question Answering
This paper is an empirical study of the distributed deep learning for
question answering subtasks: answer selection and question classification.
Comparison studies of SGD, MSGD, ADADELTA, ADAGRAD, ADAM/ADAMAX, RMSPROP,
DOWNPOUR and EASGD/EAMSGD algorithms have been presented. Experimental results
show that the distributed framework based on the message passing interface can
accelerate the convergence speed at a sublinear scale. This paper demonstrates
the importance of distributed training. For example, with 48 workers, a 24x
speedup is achievable for the answer selection task and running time is
decreased from 138.2 hours to 5.81 hours, which will increase the productivity
significantly.Comment: This paper will appear in the Proceeding of The 25th ACM
International Conference on Information and Knowledge Management (CIKM 2016),
Indianapolis, US
Continuum Derrida Approach to Drift and Diffusivity in Random Media
By means of rather general arguments, based on an approach due to Derrida
that makes use of samples of finite size, we analyse the effective diffusivity
and drift tensors in certain types of random medium in which the motion of the
particles is controlled by molecular diffusion and a local flow field with
known statistical properties. The power of the Derrida method is that it uses
the equilibrium probability distribution, that exists for each {\em finite}
sample, to compute asymptotic behaviour at large times in the {\em infinite}
medium. In certain cases, where this equilibrium situation is associated with a
vanishing microcurrent, our results demonstrate the equality of the
renormalization processes for the effective drift and diffusivity tensors. This
establishes, for those cases, a Ward identity previously verified only to
two-loop order in perturbation theory in certain models. The technique can be
applied also to media in which the diffusivity exhibits spatial fluctuations.
We derive a simple relationship between the effective diffusivity in this case
and that for an associated gradient drift problem that provides an interesting
constraint on previously conjectured results.Comment: 18 pages, Latex, DAMTP-96-8
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