Innovaemprende

El proyecto INNOVAEMPRENDE se ha desarrollado en el contexto del Máster en Biotecnología Industrial y Ambiental, dentro de la asignatura del módulo fundamental “Organización y Seguridad Industrial” con el objetivo general de contribuir a la formación de los alumnos del Máster en la cultura del emprendimiento, favoreciendo la conciencia del valor del conocimiento dentro del ámbito de la biotecnología, sector en crecimiento que, en el contexto global de una economía cada vez más fundamentada en la I+D+i, ha demostrado su importancia e impacto económico, siendo uno de los nuevos yacimientos de riqueza económica y de empleo

Kinetically controlled acylation of 6-APA catalyzed by penicillin acylase from Streptomyces lavendulae: effect of reaction conditions in the enzymatic synthesis of penicillin V

Enzymatic synthesis of penicillin V (penV) by acylation of 6-aminopenicillanic acid (6-APA) was carried out using methyl phenoxyacetate (MPOA) as activated acyl donor and soluble penicillin acylase from Streptomyces lavendulae (SlPVA) as biocatalyst. The effect of different reaction conditions on penV synthesis was investigated, such as enzyme concentration, pH, molar ratio of 6-APA to MPOA, as well as presence of DMSO as water-miscible co-solvent at different concentrations. Time-course profiles of all reactions followed the typical pattern of kinetically controlled synthesis (KCS) of β-lactam antibiotics: penV concentration reached a maximum (highest yield or Ymax) and then decreased gradually. Such maximum was higher at pH 7.0, observing that final penV concentration was abruptly reduced when basic pH values were employed in the reaction. Under the selected conditions (100 mM Tris/HCl buffer pH 7.0, 30 °C, 2.7% (v/v) DMSO, 20 mM MPOA, 0.3 UI/ml of SlPVA), Ymax was enhanced by increasing the substrate molar ratio (6-APA to MPOA) up to 5, reaching a maximum of 94.5% and a S/H value of 16.4 (ratio of synthetic activity to hydrolytic activity). As a consequence, the use of an excess of 6-APA as nucleophile has allowed us to obtain some of the highest Ymax and S/H values among those reported in literature for KCS of β-lactam antibiotics. Although many penicillin G acylases (PGAs) have been described in kinetically controlled acylations, SlPVA should be considered as a different enzyme in the biocatalytic tool-box for novel potential synthetic processes, mainly due to its different substrate specificity compared to PGAs

Development of active learning strategies in Biochemistry Laboratory BBM1

Con objeto de fomentar el aprendizaje activo en el laboratorio de Bioquímica BBM1, se diseñaron, emplearon y evaluaron una serie de actividades y herramientas: (i) test pre-laboratorio, realizados mediante la aplicación Socrative; (ii) herramientas de apoyo al aprendizaje autónomo del estudiante, relacionadas con la elaboración del cuaderno de laboratorio y los procesos de evaluación; (iii) actividades formativas complementarias, tales como el desarrollo de un mini-congreso y una visita a las instalaciones del Departamento. El objetivo final del proyecto es que el estudiante entienda de verdad lo que hace en el laboratorio y sea capaz de aplicar lo que ha aprendido a nuevas situaciones.In order to promote active learning in the BBM1 Biochemistry laboratory, several activities and tools were designed, used and evaluated: (i) pre-laboratory tests, carried out using Socrative application; (ii) tools to support the student's autonomous learning, related to preparation of the laboratory notebook and evaluation processes; (iii) supplementary training activities, such as development of a mini-congress and a visit to the Department's facilities. The ultimate goal of the project is that the student really understands what he does in the laboratory and is able to apply what he has learned to new situations.Depto. de Bioquímica y Biología MolecularFac. de Ciencias QuímicasFALSEsubmitte