Calidad de los efluentes de las Plantas de Tratamiento de Aguas Residuales domésticas de Piura

The article’s objective was to evaluate the quality of the domestic effluents of the Wastewater Treatment Plants (WWTP) in the city of Piura between the years 2019 to 2021. The methodology used was the quantitative method, for later, it analysed the statistical data of the Effluents Quality Evaluation of the Providers of Sanitation Services annual reports. It is so which evaluated the quality of the effluents from the WWTPs over the years at interest. Collected results indicated a higher level of non-compliance at the following parameters: Thermotolerant Coliforms (93.10%), Total Suspended Solids (41.38%), Chemical Oxygen Demand (79.31%), Biological Oxygen Demand (65 .52%), Oils and Fats (10.34%), Ph (6.90%) and Temperature (0.00%). In conclusion, it proved that the values do not comply with the established Maximum Permissible Limits, exceeding a percentage greater than 40% in 4/7 evaluation parameters.La presente investigación tuvo por objetivo evaluar la calidad de los efluentes de las Plantas de Tratamiento de Aguas Residuales (PTAR) domésticas del departamento de Piura en el periodo 2019 - 2021. Se hizo uso de una metodología cuantitativa y se analizó la información de los datos estadísticos procedentes de los informes anuales de la Evaluación de la Calidad de los efluentes de los Prestadores de Servicios de Saneamiento, mediante la cual, se logró evaluar la calidad de los efluentes de las PTAR en los años de interés. Los resultados obtenidos indicaron un mayor nivel de incumplimiento en los siguientes parámetros: Coliformes Termotolerantes (93,10%), Sólidos Suspendidos Totales (41,38%), Demanda Química de Oxígeno (79,31%), Demanda Biológica de Oxígeno (65,52%), Aceites y Grasas (10,34%), pH (6,90%) y Temperatura (0,00%). En conclusión, se evidenció que los valores no cumplen con los Límites Máximos Permisibles establecidos, sobrepasando un porcentaje mayor al 40% en 4 de los 7 parámetros de evaluación

Reproducibility of fluorescent expression from engineered biological constructs in E. coli

We present results of the first large-scale interlaboratory study carried out in synthetic biology, as part of the 2014 and 2015 International Genetically Engineered Machine (iGEM) competitions. Participants at 88 institutions around the world measured fluorescence from three engineered constitutive constructs in E. coli. Few participants were able to measure absolute fluorescence, so data was analyzed in terms of ratios. Precision was strongly related to fluorescent strength, ranging from 1.54-fold standard deviation for the ratio between strong promoters to 5.75-fold for the ratio between the strongest and weakest promoter, and while host strain did not affect expression ratios, choice of instrument did. This result shows that high quantitative precision and reproducibility of results is possible, while at the same time indicating areas needing improved laboratory practices.Peer reviewe