Contribution of the genetic background to the immune response of broilers vaccinated or challenged with LPAI H9N2

<p>Abstract</p> <p>Background</p> <p>The knowledge on the immune responses to LPAI is limited. The purpose of this study was to investigate the immune responses of two divergently selected lines of broilers, a line responding with high antibody response to antigens (HH), and a line responding with low antibody titers (LL) to antigen.</p> <p>Methods</p> <p>Day old chicks from each line were divided in two groups, one vaccinated with inactivated H9N2 vaccine and one non-vaccinated. At 21 days of age all the chicks were challenged with field isolate of H9N2, 1X10^6.5 ELD₅₀ per chick by drops to the eye, nose and beak. Twenty four hours and 14 days post challenge (PC), the chickens were weighed blood spleen and lungs were taken and leukocytes were isolated. The leukocytes were stained with monoclonal antibodies for surface markers and analyzed by flow cytometry. We used Elispot assay to identify the number of antibody producing cells in each of the organs. mRNA was extracted using TRIsol reagent in order to assess the cytokine production level by qRT-PCR using the SYBR green methods.</p> <p>Results</p> <p>Our results showed that LL-vaccinated group gained more weight than any of the other group. Using IDEXX kit, no antibody titers could be identified in vaccinated chicks 21 days post vaccination while 14 days PC vaccinated HH chickens demonstrated the highest average antibody titers. LL vaccinated chickens demonstrated higher average antibody titer than non-vaccinated LL. Using the Elispot assay no difference were found between the groups either cells producing IgA, IgM or IgY beside of a high number of IgY producing cells in the lungs of vaccinated HH birds.</p> <p>Conclusions</p> <p>Further data on leukocytes subpopulations using flow cytometry, cytokines production (IFNγ, IL-6, IL-18, IL-2 and IL-4) isotype specific antibody responses and number and functionality of NK cells are in process.</p

Discovery of a Wide Low-mass Binary System in Upper Scorpius

Using the near-infrared spectrometer SpeX and its slit-viewing camera at the IRTF, I have resolved a low-mass member of the Upper Scorpius OB association into a double star. From K-band images of the pair, DENIS-P J161833.2-251750.4 A and B, I measure a separation of 0.96" and a magnitude difference of dK=0.42 mag. I present resolved 0.8-2.5 micron spectroscopy of the two objects, both of which exhibit signatures of youth in the shape of their H- and K-band continua, demonstrating that both are members of Upper Scorpius rather than field stars. In addition, through a comparison to optically-classified pre-main-sequence objects, I derive a spectral type near M5 for each component, corresponding to a mass of ~0.15 Msun with the evolutionary models of Chabrier and Baraffe. The probability that this pair is composed of unrelated M-type members of Upper Scorpius is ~10^-5. When added to the recent discoveries of other wide, easily disrupted low-mass binaries, this new system further establishes that the formation of low-mass stars and brown dwarfs does not require ejection from multiple systems. These observations also indicate that wide low-mass binaries can form in OB associations as well as in smaller clusters where the previously known wide pairs have been found. Thus, the available data show no perceptible effect of star-forming environment on the prevalence of loosely bound low-mass systems.Comment: 5 page

Mid-infrared Imaging of a Circumstellar Disk Around HR 4796: Mapping the Debris of Planetary Formation

We report the discovery of a circumstellar disk around the young A0 star, HR 4796, in thermal infrared imaging carried out at the W.M. Keck Observatory. By fitting a model of the emission from a flat dusty disk to an image at lambda=20.8 microns, we derive a disk inclination, i = 72 +6/-9 deg from face on, with the long axis of emission at PA 28 +/-6 deg. The intensity of emission does not decrease with radius as expected for circumstellar disks but increases outward from the star, peaking near both ends of the elongated structure. We simulate this appearance by varying the inner radius in our model and find an inner hole in the disk with radius R_in = 55+/-15 AU. This value corresponds to the radial distance of our own Kuiper belt and may suggest a source of dust in the collision of cometesimals. By contrast with the appearance at 20.8 microns, excess emission at lambda = 12.5 microns is faint and concentrated at the stellar position. Similar emission is also detected at 20.8 microns in residual subtraction of the best-fit model from the image. The intensity and ratio of flux densities at the two wavelengths could be accounted for by a tenuous dust component that is confined within a few AU of the star with mean temperature of a few hundred degrees K, similar to that of zodiacal dust in our own solar system. The morphology of dust emission from HR 4796 (age 10 Myr) suggests that its disk is in a transitional planet-forming stage, between that of massive gaseous proto-stellar disks and more tenuous debris disks such as the one detected around Vega.Comment: 9 pages, 4 figures as LaTex manuscript and postscript files in gzipped tar file. Accepted for publication in Astrophysical Journal Letters. http://upenn5.hep.upenn.edu/~davidk/hr4796.htm

Sensitive Limits on the Water Abundance in Cold Low Mass Molecular Cores

We present SWAS observations of water vapor in two cold star-less clouds, B68 and Core D in rho Ophiuchus. Sensitive non-detections of the 1(10)-1(01) transition of o-H2O are reported for each source. Both molecular cores have been previously examined by detailed observations that have characterized the physical structure. Using these rather well defined physical properties and a Monte-Carlo radiation transfer model we have removed one of the largest uncertainties from the abundance calculation and set the lowest water abundance limit to date in cold low-mass molecular cores. These limits are < 3 x 10^{-8} (relative to H2) and < 8 x 10^{-9} in B68 and rho Oph D, respectively. Such low abundances confirm the general lack of ortho-water vapor in cold (T < 20 K) cores. Provided that the ortho/para ratio of water is not near zero, these limits are well below theoretical predictions and appear to support the suggestion that most of the water in dense low-mass cores is frozen onto the surfaces of cold dust grains.Comment: 12 pages, 3 figures, accepted by Astrophysical Journal Letter

Quiescent Dense Gas in Protostellar Clusters: the Ophiuchus A Core

We present combined BIMA interferometer and IRAM 30 m Telescope data of N2H+ 1-0 line emission across the nearby dense, star forming core Ophiuchus A (Oph A) at high linear resolution (e.g., ~1000 AU). Six maxima of integrated line intensity are detected which we designate Oph A-N1 through N6. The N4 and N5 maxima are coincident with the starless continuum objects SM1 and SM2 respectively but the other maxima are not coincident with previously-identified objects. In contrast, relatively little N2H+ 1-0 emission is coincident with the starless object SM2 and the Class 0 protostar VLA 1623. The FWHM of the N2H+ 1-0 line, Delta V, varies by a factor of ~5 across Oph A. Values of Delta V < 0.3 km/s are found in 14 locations in Oph A, but only that associated with N6 is both well-defined spatially and larger than the beam size. Centroid velocities of the line, V_LSR, vary relatively little, having an rms of only \~0.17 km/s. Small-scale V_LSR gradients of <0.5 km/s over ~0.01 pc are found near SM1, SM1N, and SM2, but not N6. The low N2H+ abundances of SM2 or VLA 1623 relative to SM1, SM1N, or N6 may reflect relatively greater amounts of N2 adsorption onto dust grains in their colder and probably denser interiors. The low Delta V of N6, i.e., 0.193 km/s FWHM, is only marginally larger than the FWHM expected from thermal motions alone, suggesting turbulent motions in the Oph A core have been reduced dramatically at this location. The non-detection of N6 in previous thermal continuum maps suggests that interesting sites possibly related to star formation may be overlooked in such data.Comment: LaTex with 7 figures, produces 36 pages. Accepted for publication in ApJ. Typo related to Equation 3 fixed, caused derived values of N(N2H+) and X(N2H+) to be low by factors of ~40%. Conclusions of paper are unchange

Current Star Formation in the Ophiuchus and Perseus Molecular Clouds: Constraints and Comparisons from Unbiased Submillimeter and Mid-Infrared Surveys. II

We present a census of the population of deeply embedded young stellar objects (YSOs) in the Ophiuchus molecular cloud complex based on a combination of Spitzer Space Telescope mid-infrared data from the "Cores to Disks" (c2d) legacy team and JCMT/SCUBA submillimeter maps from the COMPLETE team. We have applied a method developed for identifying embedded protostars in Perseus to these datasets and in this way construct a relatively unbiased sample of 27 candidate embedded protostars with envelopes more massive than our sensitivity limit (about 0.1 M_sun). Embedded YSOs are found in 35% of the SCUBA cores - less than in Perseus (58%). On the other hand the mid-infrared sources in Ophiuchus have less red mid-infrared colors, possibly indicating that they are less embedded. We apply a nearest neighbor surface density algorithm to define the substructure in each of the clouds and calculate characteristic numbers for each subregion - including masses, star formation efficiencies, fraction of embedded sources etc. Generally the main clusters in Ophiuchus and Perseus (L1688, NGC1333 and IC348) are found to have higher star formation efficiencies than small groups such as B1, L1455 and L1448, which on the other hand are completely dominated by deeply embedded protostars. We discuss possible explanations for the differences between the regions in Perseus and Ophiuchus, such as different evolutionary timescales for the YSOs or differences, e.g., in the accretion in the two clouds.Comment: Accepted for publication in ApJ (56 pages, 13 figures; abstract abridged). Version with full-resolution figures available at http://www.astro.uni-bonn.de/~jes/paper120.pd

High Accretion Rate during Class 0 Phase due to External Trigger

Recent observations indicate that some class 0 sources have orders of magnitude higher accretion rates than those of class I. We investigated the conditions for the high accretion rates of some class 0 sources by numerical calculations, modelling an external trigger. For no external trigger, we find that the maximum value of the accretion rate is determined by the ratio $\alpha$ of the gravitational energy to the thermal one within a flat inner region of the cloud core. The accretion rate reaches \sim 10^{-4} M_{\sun} yr^{-1} if the cloud core has $\alpha > 2$. For an external trigger we find that the maximum value of the accretion rate is proportional to the momentum given to the cloud core. The accretion rate reaches > 10^{-4} M_{\sun} yr^{-1} with a momentum of \sim 0.1 M_{\sun} km s^{-1} when the initial central density of the cloud core is $\sim 10^{-18} g cm^{-3}$. A comparison between recent observational results for prestellar cores and our no triggered collapse model indicates that the flat inner regions of typical prestellar cores are not large enough to cause accretion rates of \sim 10^{-4} M_{\sun} yr^{-1}. Our results show that the triggered collapse of the cloud core is more preferable for the origin of the high accretion rates of class 0 sources than no triggered collapse.Comment: 7 pages, 8 figures, accepted for publication in MNRA