Plan de negocio para la implementaci?n de una empresa de venta multicanal de productos tecnol?gicos inform?ticos en Piura

La propuesta surge ante la potencialidad de un sector din?mico en dos mercados identificables: personas naturales y empresas. Piura es la segunda ciudad m?s poblada; la investigaci?n de mercado revela un mercado potencial de m?s de 300,000 personas s?lo en la poblaci?n urbana de los 2 distritos m?s importantes y m?s de 10,000 Mypes en la provincia de Piura. El modelo de negocio desarrolla una propuesta de valor que se enfoca en la entrega de productos novedosos, eficientes y de calidad. La estrategia se fundamenta en la experiencia del cliente e innovaci?n de productos. Marketing se fija objetivos de crecimiento y cuota de mercado incremental a lograr con el posicionamiento de la marca, basado en variables de precio, calidad y orientaci?n/asistencia. Operaciones usa estrategia de costos y flexibilidad y gestiona el proceso de importaci?n con sus dos canales la tienda presencial y la plataforma e-commerce. Recursos humanos establece un organigrama b?sico cuyo personal incrementa progresivamente de acuerdo al crecimiento del negocio. La evaluaci?n financiera obtiene un VANF de S/. 29,761 y TIRF de 23.53% por encima de su COK de 21.7%. El negocio se muestra atractivo sin perder de vista variables sensibles que deben ser monitorizadas para lograr el ?xito

Databases as policy instruments. About extending networks as evidence-based policy

Background. This article seeks to identify the role of databases in health policy. Access to information and communication technologies has changed traditional relationships between the state and professionals, creating new systems of surveillance and control. As a result, databases may have a profound effect on controlling clinical practice. Methods. We conducted three case studies to reconstruct the development and use of databases as policy instruments. Each database was intended to be employed to control the use of one particular pharmaceutical in the Netherlands (growth hormone, antiretroviral drugs for HIV and Taxol, respectively). We studied the archives of th

Ultrastructural and functional fate of recycled vesicles in hippocampal synapses

Efficient recycling of synaptic vesicles is thought to be critical for sustained information transfer at central terminals. However, the specific contribution that retrieved vesicles make to future transmission events remains unclear. Here we exploit fluorescence and time-stamped electron microscopy to track the functional and positional fate of vesicles endocytosed after readily releasable pool (RRP) stimulation in rat hippocampal synapses. We show that most vesicles are recovered near the active zone but subsequently take up random positions in the cluster, without preferential bias for future use. These vesicles non-selectively queue, advancing towards the release site with further stimulation in an actin-dependent manner. Nonetheless, the small subset of vesicles retrieved recently in the stimulus train persist nearer the active zone and exhibit more privileged use in the next RRP. Our findings reveal heterogeneity in vesicle fate based on nanoscale position and timing rules, providing new insights into the origins of future pool constitution

Magnetization relaxation in the single molecule magnet Ni4_4 under continuous microwave irradiation

Spin relaxation between the two lowest-lying spin-states has been studied in the S=4 single molecule magnet Ni$_4$ under steady state conditions of low amplitude and continuous microwave irradiation. The relaxation rate was determined as a function of temperature at two frequencies, 10 and 27.8 GHz, by simultaneously measuring the magnetization and the absorbed microwave power. A strong temperature dependence is observed below 1.5 K, which is not consistent with a direct single-spin-phonon relaxation process. The data instead suggest that the spin relaxation is dominated by a phonon bottleneck at low temperatures and occurs by an Orbach mechanism involving excited spin-levels at higher temperatures. Experimental results are compared with detailed calculations of the relaxation rate using the universal density matrix equation.Comment: 6 pages, 4 figures, submitted to Europhys. Let

Workplace flexibility across the lifespan

As demographic changes impact the workplace, governments, organizations and workers are looking for ways to sustain optimal working lives at higher ages. Workplace flexibility has been introduced as a potential way workers can have more satisfying working lives until their retirement ages. This paper presents a critical review of the literature on workplace flexibility across the lifespan. It discusses how flexibility has been conceptualized across different disciplines, and postulates a definition that captures the joint roles of employer and employee in negotiating workplace flexibility that contributes to both employee and organization benefits. Moreover, it reviews how flexibility has been theorized and investigated in relation to older workers. The paper ends with a future research agenda for advancing understanding of how workplace flexibility may enhance working experiences of older workers, and in particular focuses on the critical investigation of uses of flexibility in relation to older workers

Climbing Fiber Burst Size and Olivary Sub-threshold Oscillations in a Network Setting

The inferior olivary nucleus provides one of the two main inputs to the cerebellum: the so-called climbing fibers. Activation of climbing fibers is generally believed to be related to timing of motor commands and/or motor learning. Climbing fiber spikes lead to large all-or-none action potentials in cerebellar Purkinje cells, overriding any other ongoing activity and silencing these cells for a brief period of time afterwards. Empirical evidence shows that the climbing fiber can transmit a short burst of spikes as a result of an olivary cell somatic spike, potentially increasing the information being transferred to the cerebellum per climbing fiber activation. Previously reported results from in vitro studies suggested that the information encoded in the climbing fiber burst is related to the occurrence of the spike relative to the ongoing sub-threshold membrane potential oscillation of the olivary cell, i.e. that the phase of the oscillation is reflected in the size of the climbing fiber burst. We used a detailed three-compartmental model of an inferior olivary cell to further investigate the possible factors determining the size of the climbing fiber burst. Our findings suggest that the phase-dependency of the burst size is present but limited and that charge flow between soma and dendrite is a major determinant of the climbing fiber burst. From our findings it follows that phenomena such as cell ensemble synchrony can have a big effect on the climbing fiber burst size through dendrodendritic gap-junctional coupling between olivary cells

Quantification of the groundwater-surface water interaction by analysing temperature gradients in the streambed of the Aa river, Belgium

The aim of this research is to gain better insight in the diverse physical and biological processes in margins, inundation areas and the hyporheic zone of water courses on a local scale. Data and results presented are from a multidisciplinary study on exchange processes in river ecosystems in which biologists, hydrologists, ecologists and engineers cooperate.Groundwater and eco-biochemical models are integrated in order to determine the exchange of water, dissolved compounds and particulate matter. Several of these processes occur simultaneously and result in feedback to other processes; hence most of the investigations aim to determine net rates of exchange. GIS is used for data management, while FEMME (Soetaert et al., 2002) serves as a platform for the integration of the different models such as MODFLOW, DAFLOW, Delft3D, and WetSpa.An innovative but cost-effective method for field investigation is the measurement of streambed temperatures profiles, which lead to a delineation and quantification of the groundwater discharge on a local scale. For the Aa River site in Belgium, a typical low land river system, combinations of longitudinal and cross-sectional measurements of temperature profiles have been conducted. Along a 1400 m long section, 5 vertical measurements of up to 80-100 cm deep into the river bottom have been performed bi-monthly between August 2004 and February 2007 at 14 measurement points and 5 cross-sections. With this information we assessed the vertical component of the groundwater flux and the spatial and temporal variability of the groundwater-surface water exchange.A streambed temperature survey does not lead directly to an estimation of the groundwater flux. Additional information, especially thermodynamic parameters of the soilwater matrix are necessary.The groundwater fluxes were calculated as point values at the measurement locations on basis of an analytical solution presented by Arriaga et al. (2006), and which is solved with the help of Microsoft Excel Solver and MATLAB. The fundamental heat flow equation was also introduced in a diagenetic model, setup in FEMME and used to compare with the Arriaga et al. (2006) solution. Interpolation of the point estimates results in net groundwater fluxes on the scale of the surveyed area. These results show discharge as well as recharge dependent on the location along the section and the season. The upper reach shows in general higher discharge rates and no change in direction of the groundwater flow, whereas the lower reach is characterized by lower flow rates and a change of direction of flow

Pre-M Phase-promoting Factor Associates with Annulate Lamellae in Xenopus Oocytes and Egg Extracts

We have used complementary biochemical and in vivo approaches to study the compartmentalization of M phase-promoting factor (MPF) in prophase Xenopus eggs and oocytes. We first examined the distribution of MPF (Cdc2/CyclinB2) and membranous organelles in high-speed extracts of Xenopus eggs made during mitotic prophase. These extracts were found to lack mitochondria, Golgi membranes, and most endoplasmic reticulum (ER) but to contain the bulk of the pre-MPF pool. This pre-MPF could be pelleted by further centrifugation along with components necessary to activate it. On activation, Cdc2/CyclinB2 moved into the soluble fraction. Electron microscopy and Western blot analysis showed that the pre-MPF pellet contained a specific ER subdomain comprising "annulate lamellae" (AL): stacked ER membranes highly enriched in nuclear pores. Colocalization of pre-MPF with AL was demonstrated by anti-CyclinB2 immunofluorescence in prophase oocytes, in which AL are positioned close to the vegetal surface. Green fluorescent protein-CyclinB2 expressed in oocytes also localized at AL. These data suggest that inactive MPF associates with nuclear envelope components just before activation. This association may explain why nuclei and centrosomes stimulate MPF activation and provide a mechanism for targeting of MPF to some of its key substrates