THE STUDY OF THE ACUTE AND SUB-ACUTE ORAL TOXICITIES OF THE NEBULIZED EXTRACT OF MYRACRODRUON URUNDEUVA ALLEMÃO IN RABBITS

Objective: The aim of this research was to evaluate the acute and sub-acute oral toxicities of the nebulized dried extract of Myracrodruon urundeuva (NDEMU) leaf obtained by the spray drying technique on rabbits.Methods: In the acute toxicity study, the amount of nebulized dried extract (NDE) administered was adjusted to a dose of 2000 mg/kg of leaf powder of M. urundeuva to 6 rabbits once orally and were observed for 14 days. In the sub-acute study, the amount of NDEMU administered was adjusted to a dose of 2000 mg/kg/day of to 6 rabbits once daily for 30 day, orally. The appearance of toxic symptoms was observed every day, followed by each rabbits' food and drink intake. Haematological and biochemical analysis were observed and statistical analysis was performed on them. The rabbits were killed at the end of the study, and their organs were weighed and examined before organ histology were evaluated.Results: No toxic signs and no mortality were observed in the acute and sub-acute study. In the sub-acute study, the amount of dried extract administered was adjusted to a dose of 2000 mg/kg of leaf powder of M. urundeuva to 6 rabbits once daily for 30 days, orally. No toxic signs and no mortality were observed. There were no significant changes (p < 0.05) in the body weights, organ weights and haemato-biochemical parameters in any of the dose levels. No related histopathological lesions were observed.Conclusion: The results indicate that the treatment of repeated doses with the dried NDEME showed low toxicity in rabbits

STANDARDIZATION AND STABILITY EVALUATION OF DRY EXTRACTS OF MYRACRODRUON URUNDEUVA ALLEMÃO OBTAINED BY SPRAY DRIER

Objective: This study aimed to obtain standardised dry extracts of Miracrodruon urundeuva Allemão using spray-dryer and evaluate the stability of the extracts.Methods: It evaluated the drying parameters: Proportion of colloidal silicon dioxide (CSD) (10, 15 and 20%), inlet temperature (160, 170 and 180 °C) and feed rate (4, 6 and 8 ml/min). The study of the accelerated stability of dry extract occurred in temperature of 40 °C (±2 °C) and relative humidity of 75% (±5%) for 6 mo. The anti-inflammatory activity of the dry extract was evaluated in Swiss mice by the paw edema method.Results: Variations in drying conditions did not represent significant variations in yields of the process. The drying temperature and feed rate significantly influenced the concentration of quercetin (p≤0.05). The increase in inlet temperature and feed flow promoted the increase of quercetin concentration in the extracts. The stability study showed that the concentration of quercetin in dry extract was stable over a period of 6 mo. The dry extract showed anti-inflammatory activity in mice orally.Conclusion: A condition of 10% of colloidal silicon dioxide with an 180 °C inlet temperature and a feed rate of 8 ml/min was considered the most adequate for obtaining the extracts and the drying process resulted in stable dry extracts and the quercetin was a suitable biomarker for monitoring the process