Influence of Pre-Heating Regular Resin Composites and Flowable Composites on Luting Ceramic Veneers with Different Thicknesses

The aim of this study was to evaluate of pre-heating, filler contents and ceramic thickness on film thickness, microshear bond strength, degree of conversion and color change on ceramic veneers. Two experimental composites were prepared (Bis-GMA/UDMA/BisEMA/ TEGDMA), with different amounts of filler (65% or 50%wt) simulating a conventional and a flowable composite. The flowable (F) was used at room temperature and, the conventional either at room temperature (C) or pre-heated (CPH). Disk-shaped ceramics with different thickness (0.4 mm, 0.8 mm, 1.5 mm) were prepared. The film thickness was evaluated according to the ISO 4049 (n=10). The microshear bond strength (n=10) was evaluated in enamel using tubing specimens light-cured through the ceramic veneer. The degree of conversion was evaluated using Raman spectroscopy. The color change of the ceramic restorations (n=10) was evaluated by spectrophotometry. The results were submitted to 2-way ANOVA and Tukey’s post hoc test (a=5%). For the film thickness 1-way ANOVA was used (a=5%). The C presented the thicker film thickness; the CPH produced a similar film thickness in comparison to the F. All composites showed similar microshear bond strength. The degree of conversion of the F was higher than the C and CPH. The degree of conversion of the composites photo-activated through a 0.4 mm was higher than the composites photo-activated through thicker ceramics. The C showed the highest color change, while the CPH showed similar color change to the F. In conclusion, pre-heated conventional composites seem to be a potential alternative to lute ceramic veneers such as the flowable composites

Manejo de açaízais como elemento de mudança comportamental em busca da soberania alimentar de uma comunidade indígena na Amazônia

O presente estudo teve impulso a partir da análise das necessidades que a Comunidade Indígena da Aldeia IarapeIw’azu, da etnia Tembé, apresentou nas visitas técnicas, encadeando reflexões sobre o melhor aproveitamento e uso dos recursos naturais presentes na área, principalmente para o cultivo do açaí (Euterpe Oleracea Mart.), cujo produto é a principal fonte de renda e a base alimentar da aldeia. Está localizada em uma área de várzea do Nordeste Paraense no Alto do Rio Guamá, a qual faz parte de um projeto de extensão da Universidade Federal Rural da Amazônia, Campus Capitão Poço-PA, e teve como colaboradores técnicos do Serviço Nacional de Aprendizagem Rural/SENAR, bolsistas do referido projeto e professores. As visitas ocorreram no mês de julho de 2014, revelando que a falta do fruto açaí era a principal problemática da comunidade, assim, buscou-se instruir os indígenas na realização do manejo dos açaizais iniciado no mês de março de 2015.Eje: B5 Sistemas de conocimiento (Relatos de experiencias)Facultad de Ciencias Agrarias y Forestale

Manejo de açaízais como elemento de mudança comportamental em busca da soberania alimentar de uma comunidade indígena na Amazônia

O presente estudo teve impulso a partir da análise das necessidades que a Comunidade Indígena da Aldeia IarapeIw’azu, da etnia Tembé, apresentou nas visitas técnicas, encadeando reflexões sobre o melhor aproveitamento e uso dos recursos naturais presentes na área, principalmente para o cultivo do açaí (Euterpe Oleracea Mart.), cujo produto é a principal fonte de renda e a base alimentar da aldeia. Está localizada em uma área de várzea do Nordeste Paraense no Alto do Rio Guamá, a qual faz parte de um projeto de extensão da Universidade Federal Rural da Amazônia, Campus Capitão Poço-PA, e teve como colaboradores técnicos do Serviço Nacional de Aprendizagem Rural/SENAR, bolsistas do referido projeto e professores. As visitas ocorreram no mês de julho de 2014, revelando que a falta do fruto açaí era a principal problemática da comunidade, assim, buscou-se instruir os indígenas na realização do manejo dos açaizais iniciado no mês de março de 2015.Eje: B5 Sistemas de conocimiento (Relatos de experiencias)Facultad de Ciencias Agrarias y Forestale

Manejo de açaízais como elemento de mudança comportamental em busca da soberania alimentar de uma comunidade indígena na Amazônia

O presente estudo teve impulso a partir da análise das necessidades que a Comunidade Indígena da Aldeia IarapeIw’azu, da etnia Tembé, apresentou nas visitas técnicas, encadeando reflexões sobre o melhor aproveitamento e uso dos recursos naturais presentes na área, principalmente para o cultivo do açaí (Euterpe Oleracea Mart.), cujo produto é a principal fonte de renda e a base alimentar da aldeia. Está localizada em uma área de várzea do Nordeste Paraense no Alto do Rio Guamá, a qual faz parte de um projeto de extensão da Universidade Federal Rural da Amazônia, Campus Capitão Poço-PA, e teve como colaboradores técnicos do Serviço Nacional de Aprendizagem Rural/SENAR, bolsistas do referido projeto e professores. As visitas ocorreram no mês de julho de 2014, revelando que a falta do fruto açaí era a principal problemática da comunidade, assim, buscou-se instruir os indígenas na realização do manejo dos açaizais iniciado no mês de março de 2015.Eje: B5 Sistemas de conocimiento (Relatos de experiencias)Facultad de Ciencias Agrarias y Forestale

Mapeamento cartográfico do município de Paulo Jacinto-AL

The present study aims to confect the basemap of Paulo Jacinto county in the state of Alagoas. Technologies of Remote Sensing and Geographic Information System-GIS were used in the process, which enabled the manufacturing of a geographic database with matrix and vector information. This way, it is possible to perform the correlations between different data models in a single virtual environment, which maximizes the quality of the products originated in these interrelationships. Thematic maps were made regarding: hypsometry, drainage, vegetation, geology, soils, climate, isohyets, and isotherms. For possessing such material, the city of Paulo Jacinto-Al now has cartographic materials that facilitated decision making for implementation of public policies that are in line with sustainable development.O presente trabalho tem como objetivo confeccionar a base cartográfica do Município de Paulo Jacinto-AL, para isso foram utilizadas as tecnologias do Sensoriamento Remoto e Sistema de Informações Geográficas-SIG, as quais possibilitaram a confecção de um banco de dados geográficos com informações matriciais e vetoriais. Tornando possível realizar as correlações entre os distintos modelos de dados em um mesmo ambiente virtual, o que maximiza a qualidade dos produtos oriundos dessas inter-relações. Dessa forma foram confeccionadas as cartas temáticas referentes: hipsometria, drenagem, cobertura vegetal, geologia, solos, clima, isoietas, isotermas... De posse desse material o município de Paulo Jacinto-Al passou a ter materiais cartográficos que facilitaram a tomada de decisões para implementação de políticas públicas que estejam em consonância com o desenvolvimento sustentável

O perfil epidemiológico de gestantes atendidas nas unidades básicas de saúde de Gurupi, Tocantins

Este artigo propôs-se a traçar o perfil clínico e sociodemográfico das gestantes atendidas pelo SUS (Serviço Único de Saúde) no Município de Gurupi, Tocantins. Foi feita uma análise retrospectiva nas UBS (Unidades Básicas de Saúde) da cidade. Foram avaliadas 6583 gestantes e selecionadas 1049 mulheres. Foram coletados dados demográficos, sociais e clínicos. Os dados foram tabulados no programa Epi-Info 3.3.2 sendo aplicada estatística descritiva com IC95%. Foi observado que 47,0% não se submeteram a seis consultas pré-natais, 83,1% eram negras ou pardas e 46,4% não tinham profissão. Foi encontrada toxoplasmose aguda em 5,0% (52/1049), sífilis em 3,6% (38/1049), hepatite B em 2,7% (28/1049), herpes simplex vírus em 2,0% (21/1049), HIV em 1,9% (20/1049), hipertensão em 15,6% (164/1049), doença neurológica em 1,2% (13/1049) e diabetes em 1,1% (12/1049), cefaléia 38%, dor no baixo ventre 35,5% e leucorreia 19,2%. Concluímos que a prevalência de doenças infecciosas e não infecciosas nas gestantes foi alta

Lung-Digit Syndrome Related to an Adenosquamous Feline Lung Carcinoma

Background: The lung-digit syndrome is a rare syndrome characterized by the presence of a primary pulmonar neoplasia manifesting metastasis to the digit, occurring exclusively in felines. The diagnosis is based on the clinical signs, associated with radiographic and histopathological exams. There are no therapeutic protocols well-established, and surgical excision is considered controversial due to high recurrence and metastasis rates. The prognosis is considered poor, with low survival rates. The aim of this paper is to report a case of lung-digit syndrome attended in Rio de Janeiro.Case: A 12-year-old female cat, no defined race, was attend with the complaint of weight loss and injury in the right thoracic limb, already having histopathological diagnosis of squamous differentiation adenocarcinoma through biopsy. Physical examination showed no other clinical signs at first, including no signs of respiratory disease. Laboratory and imaging exams were performed, and the radiographic examination showed alterations in the pulmonary parenchyma showing a nodular area of increased radiographic density. Followed up by the manifestation of breathing noise at rest, as the first respiratory signs. The association of the clinical evaluation, medical history, and histopathological report from the limb lesion, lead to the suspicion diagnostic of lung-digit syndrome. The owners decide for the palliative treatment with chemotherapy, using carboplatin and prednisolone. Only the first session was performed, with the animal being euthanized due to clinical worsening 48 days after the initial manifestation of clinical signs. The diagnosis was confirmed by post mortem exams, and the pulmonary nodule were diagnosed as adenocarcinoma with squamous differentiation in concordance with the limb lesion diagnosis.Discussion: The lung-digit syndrome is still poorly studied in the veterinary medicine, with few reports published. Epidemiological data shows major incidence in elderly cats, with no racial or sexual predisposition, occurring in a 12-year-old cat in the present case. There are few theorys to explain the etiopathogenesis of the syndrome, however, none have been fully comproved. The clinical symptomatology varies from asymptomatic animals to those manifesting nonspecific systemic signs and respiratory signs. In this case the animal initially manifested only the presence of the digit injury and weight loss, with respiratory signs manifesting later. The digital lesion local signs usually include swelling, ulceration, purulent discharge, nail loss, and pain, occurring more frequently in the thoracic limbs, involving weight-bearing digits, as in the present report that occurred in the right thoracic limb, with involvement of the first and third digits. Radiographic findings may help in the diagnosis suspicion, having the confirmation by histopathological examination. The most frequent histological types involved in the syndrome are the adenocarcinoma, bronchoalveolar carcinoma, squamous cell carcinoma and adenosquamous carcinoma. The squamous-differentiated adenocarcinoma or adenosquamous carcinoma, as described in the histopathological report in the present case, is the least frequent. The treatment protocol is not well-established with no proven effective treatment. Surgical excision is not recomended as a palliative method, and the chemotherapy and radiotherapy sucess rates are still unknown. The prognosis is considered extremely unfavorable and early diagnosis represents the best attempt to control the disease

Effect Of 4-(n,n-dimethylamino)phenethyl alcohol on degree of conversion and cytotoxicity of photo-polymerized CQ-based resin composites

FAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOThe aim of this study was to evaluate the degree of conversion (DC) and the cytotoxicity of photo-cured experimental resin composites containing 4-(N,N-dimethylamino)phenethyl alcohol (DMPOH) combined to the camphorquinone (CQ) compared with ethylamine benzoate (EDAB). The resin composites were mechanically blended using 35 wt% of an organic matrix and 65 wt% of filler loading. To this matrix was added 0.2 wt% of CQ and 0.2 wt% of one of the reducing agents tested. 5x1 mm samples (n=5) were previously submitted to DC measurement and then pre-immersed in complete culture medium without 10% (v/v) bovine serum for 1 h or 24 h at 37 °C in a humidifier incubator with 5% CO2 and 95% humidity to evaluate the cytotoxic effects of experimental resin composites using the MTT assay on immortalized human keratinocytes cells. As a result of absence of normal distribution, the statistical analysis was performed using the nonparametric Kruskal-Wallis to evaluate the cytotoxicity and one-way analysis of variance to evaluate the DC. For multiple comparisons, cytotoxicity statistical analyses were submitted to Student-Newman-Keuls and DC analysis to Tukey's HSD post-hoc test (=0.05). No significant differences were found between the DC of DMPOH (49.9%) and EDAB (50.7%). 1 h outcomes showed no significant difference of the cell viability between EDAB (99.26%), DMPOH (94.85%) and the control group (100%). After 24 h no significant difference were found between EDAB (48.44%) and DMPOH (38.06%), but significant difference was found compared with the control group (p>0.05). DMPOH presented similar DC and cytotoxicity compared with EDAB when associated with CQ.The aim of this study was to evaluate the degree of conversion (DC) and the cytotoxicity of photo-cured experimental resin composites containing 4-(N,N-dimethylamino)phenethyl alcohol (DMPOH) combined to the camphorquinone (CQ) compared with ethylamine benzoate (EDAB). The resin composites were mechanically blended using 35 wt% of an organic matrix and 65 wt% of filler loading. To this matrix was added 0.2 wt% of CQ and 0.2 wt% of one of the reducing agents tested. 5x1 mm samples (n=5) were previously submitted to DC measurement and then pre-immersed in complete culture medium without 10% (v/v) bovine serum for 1 h or 24 h at 37 °C in a humidifier incubator with 5% CO2 and 95% humidity to evaluate the cytotoxic effects of experimental resin composites using the MTT assay on immortalized human keratinocytes cells. As a result of absence of normal distribution, the statistical analysis was performed using the nonparametric Kruskal-Wallis to evaluate the cytotoxicity and one-way analysis of variance to evaluate the DC. For multiple comparisons, cytotoxicity statistical analyses were submitted to Student-Newman-Keuls and DC analysis to Tukey's HSD post-hoc test (=0.05). No significant differences were found between the DC of DMPOH (49.9%) and EDAB (50.7%). 1 h outcomes showed no significant difference of the cell viability between EDAB (99.26%), DMPOH (94.85%) and the control group (100%). After 24 h no significant difference were found between EDAB (48.44%) and DMPOH (38.06%), but significant difference was found compared with the control group (p>0.05). DMPOH presented similar DC and cytotoxicity compared with EDAB when associated with CQ256538542FAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOFAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO2013/04241-

Validação de instrumento para o screening de adolescentes com excesso de peso na escola

The aim was to validate the content of a nursing instrument for the screening of overweight adolescents in schools. Methodological study conducted in three phases: construction of the “Overweight Screening Form for Adolescents in Schools”; psychometric evaluation by specialists; modification of instrument according to specialists’ suggestions.   The Kappa and Chi Square tests were used in the analysis. The frequency of positive responses for the psychometric criteria was ≥80%, with some agreement differences among the specialists (p<0,001). Some criteria presented low agreement (k=0,20-0,39) or no agreement (k=0). The physical evaluation item obtained a Kappa 1 in all criteria. After Form alterations were made, all criteria reported high agreement (k=1), except for “Sociodemographic profile and general data”.  The instrument was considered to have content validity and useful for the identification of nursing diagnosis of overweight adolescents. It can serve as a tool in the implementation of the nursing process with this populationEl objetivo fue validar el contenido del instrumento de enfermería para la detección de adolescentes con sobrepeso en la escuela. Estudio metodológico llevado a cabo en tres etapas: creación del “Formulario para la detección de adolescentes con sobrepeso en la escuela”; evaluación psicométrica por especialistas; modificación del instrumento de acuerdo con las recomendaciones. Las pruebas estadísticas de Kappa y Chi-Quadrado fueron utilizadas en el análisis. Las frecuencias positivas de los criterios psicométricos fueron ≥80%, con algunas diferencias de concordancia entre los especialistas (p<0,001). Algunos criterios presentaron pobre concordancia (k=0,20-0,39) y otros sin concordancia (k=0). El ítem de evaluación física tuvo valor Kappa 1 em todos los criterios. Después de hacer las alteraciones, todos los criterios tuvieron alta concordancia (K=1), con exención del ítem “Perfil sociodemográfico y datos generales”. El instrumento fue considerado de contenido válido y útil para la identificación del diagnóstico de enfermería de sobrepeso y para la implementación del proceso de enfermería en esa poblaciónObjetivou-se validar o conteúdo de instrumento para uso do enfermeiro no screening de adolescentes com excesso de peso na escola. Estudo metodológico realizado em três etapas: construção do “Formulário para o screening de adolescentes com excesso de peso na escola”; avaliação do Formulário por especialistas, segundo critérios psicométricos; modificação do Formulário conforme os pareceres. Utilizaram-se os testes Kappa e Qui-Quadrado. Os critérios psicométricos atingiram frequências ≥80% de respostas positivas, com diferença entre os especialistas (p<0,001). Alguns critérios apresentaram fraca concordância (k=0,20-0,39) ou nenhuma concordância (k<0). O item sobre avaliação física obteve valor Kappa 1 em todos os critérios. Após as alterações no Formulário, todos os critérios atingiram alta concordância (k=1), exceto o item “Perfil sociodemográfico e dados gerais”. O instrumento foi considerado válido quanto ao conteúdo e útil para a identificação do diagnóstico de enfermagem excesso de peso, podendo impactar na implementação do processo de enfermagem nessa populaçã

The inflammatory status of soluble microenvironment influences the capacity of melanoma cells to control t-cell responses

The development of immunotherapeutic approaches for the treatment of melanoma requires a better understanding of immunoescape mechanisms of tumor cells and how they interact with other tumor-resident cell types. Here, we evaluated how the conditioned media of resting (rCM) and immune-activated PBMCs (iCM) influence the ability of a metastatic melanoma cell line (MeWo) to control T-cells function. MeWo cells were expanded in RPMI, rCM, or iCM and the secretome generated after cell expansion was identified as MeSec (RPMI), niSec (non-inflammatory), or iSec (inflammatory secretome), respectively. Then, the immunomodulatory potential of such secretomes was tested in PHA-activated PBMCs. iCM induced higher levels of IFN-γ and IL-10 in treated melanoma cells compared to rCM, as well as higher IDO and PD-L1 expression. The iSec was able to inhibit T-cell activation and proliferation. Interestingly, PBMCs treated with iSec presented a reduced expression of the regulators of Th1 and Th2 responses T-BET and GATA-3, as well as low expression of IFN-γ, and co-stimulatory molecules TIM-3 and LAG-3. Importantly, our findings show that melanoma may benefit from an inflammatory microenvironment to enhance its ability to control the T-cell response. Interestingly, such an immunomodulatory effect involves the inhibition of the checkpoint molecules LAG-3 and TIM-3, which are currently investigated as important therapeutic targets for melanoma treatment. Further studies are needed to better understand how checkpoint molecules are modulated by paracrine and cell contact-dependent interaction between melanoma and immune cells. Such advances are fundamental for the development of new therapeutic approaches focused on melanoma immunotherapy