Tracking ancient beach-lines inland: 2600-year-old dentate-stamped ceramics at Hopo, Vailala River region, Papua New Guinea

The Lapita expansion took Austronesian seafaring peoples with distinctive pottery eastward from the Bismarck Archipelago to western Polynesia during the late second millennium BC, marking the first stage in the settlement of Oceania. Here it is shown that a parallel process also carried Lapita pottery and people many hundreds of kilometres westward along the southern shore of Papua New Guinea. The key site is Hopo, now 4.5km inland owing to the progradation of coastal sand dunes, but originally on the sea edge. Pottery and radiocarbon dates indicate Lapita settlement in this location c.600 BC, and suggest that the long-distance maritime networks linking the entire southern coast of Papua New Guinea in historical times may trace their origin to this period

NEW TOOLS FOR ENHANCED DIAGNOSTICS OF DGA DATA

In the last decade there has been a significant change in the way transformers are viewed. Their importance together with their obvious value to the network has been enhanced and recognized, especially in light of the ageing fleet worldwide. At the other end of the spectrum, new transformers are now being designed and built to tighter tolerances as a result of competitive market conditions, with the knock-on effect that these “modern” devices do not appear to provide the same stability and longevity as those that were entering service in the 1970s and 1980s. Against this backdrop, the advent of transformer monitoring has emerged and continues to develop at a rapid pace. Although still considered an emerging component of asset management practice, online DGA is rapidly gaining acceptance and recognition as one of the most powerful tools in protection against asset failures. While other transformer monitoring technologies abound, many of them now online, such as partial discharge, these products collectively combine to enable the move to condition based monitoring of transformer assets. As online DGA monitors have evolved new products and technologies are reaching the market at an ever increasing rate. However, the quiet revolution is in the analysis of the data. As more and more monitors are installed, so the burden of data analysis becomes increasingly large. New ways of extracting value from this data required. One important approach is the use of Artificial Neural Networks (ANN) for DGA data analysis. Additionally, with the recognition that data from monitors must be easily transferred into meaningful information for the end-user, diagnostic tools, such as the Duval Triangle, have evolved where the addition of Triangles 4 and 5 brings significantly more value to previously mined data. The mute question in this paper relates to whether or not existing online monitoring hardware has sufficient accuracy and repeatability of measurement to be of use with these more advanced diagnostic tools

Sex-linked markers in the North American green frog (Rana clamitans) developed using DArTseq provide early insight into sex chromosome evolution

BACKGROUND: The extent to which sex reversal is associated with transitions in sex determining systems (XX-XY, ZZ-ZW, etc.) or abnormal sexual differentiation is predominantly unexplored in amphibians. This is in large part because most amphibian taxa have homomorphic sex chromosomes, which has traditionally made it challenging to identify discordance between phenotypic and genetic sex in amphibians, despite all amphibians having a genetic component to sex determination. Recent advances in molecular techniques such as genome complexity reduction and high throughput sequencing present a valuable avenue for furthering our understanding of sex determination in amphibians and other taxa with homomorphic sex chromosomes like many fish and reptiles. RESULTS: We use DArTseq as a novel approach to identify sex-linked markers in the North American green frog (Rana clamitans melanota) using lab-reared tadpoles as well as wild-caught adults from seven ponds either in undeveloped, forested habitats or suburban ponds known to be subject to contamination by anthropogenic chemicals. The DArTseq methodology identified 13 sex-linked SNP loci and eight presence-absence loci associated with males, indicating an XX-XY system. Both alleles from a single locus show partial high sequence homology to Dmrt1, a gene linked to sex determination and differentiation throughout Metazoa. Two other loci have sequence similarities to regions of the chimpanzee and human X-chromosome as well as the chicken Z-chromosome. Several loci also show geographic variation in sex-linkage, possibly indicating sex chromosome recombination. While all loci are statistically sex-linked, they show varying degrees of female heterozygosity and male homozygosity, providing further evidence that some markers are on regions of the sex chromosomes undergoing higher rates of recombination and therefore further apart from the putative sex determining locus. CONCLUSION: The ease of the DArTseq platform provides a useful avenue for future research on sex reversal and sex chromosome evolution in vertebrates, particularly for non-model species with homomorphic or cryptic or nascent sex chromosomes. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12864-016-3209-x) contains supplementary material, which is available to authorized users

A climate for contemporary evolution

A new study of divergence in freshwater fish provides strong evidence of rapid, temperature-mediated adaptation. This study is particularly important in the ongoing debate over the extent and significance of evolutionary response to climate change because divergence has occurred in relatively few generations in spite of ongoing gene flow and in the aftermath of a significant genetic bottleneck, factors that have previously been considered obstacles to evolution. Climate change may thus be more likely to foster contemporary evolutionary responses than has been anticipated, and I argue here for the importance of investigating their possible occurrence

Daphniid zooplankton assemblage shifts in response to eutrophication and metal contamination during the Anthropocene

Additional results from the principal component analyses conducted on eutrophication and metals data, as well as daphniid assemblages, and species richness rarefaction

Kumukumu 1, a hilltop site in the Aird Hills: Implications for occupational trends and dynamics in the Kikori River delta, south coast of Papua New Guinea

We report on archaeological excavations undertaken at Kumukumu 1 atop the dense rainforest-clad Aird Hills of the Kikori river delta islands, south coast of Papua New Guinea. Results indicate exploitation of the nearby environment, including the gathering of some 200 million shellfish from riverine habitats at the base of the hill some 600 years ago, and deposition of shell remains onto hilltop middens. We ask what the implications of such a site in a defensive location on the upper, steep hillslope of Kumukumu hill are for regional occupation and dynamics. We conclude that the hinterland-marine fringe islands of the river deltas that include the site of Kumukumu 1 were especially sensitive to heightened cross-cultural influences and inter-group raids and competition, leading to accelerated processes of centralisation and aggrandisement among some groups, and the subjugation, fragmentation and dispersal of less powerful neighbouring groups

Age-dependent Changes in the Articular Cartilage and Subchondral Bone of C57BL/6 Mice after Surgical Destabilization of Medial Meniscus

Age is the primary risk factor for osteoarthritis (OA), yet surgical OA mouse models such as destabilization of the medial meniscus (DMM) used for evaluating disease-modifying OA targets are frequently performed on young adult mice only. This study investigates how age affects cartilage and subchondral bone changes in mouse joints following DMM. DMM was performed on male C57BL/6 mice at 4 months (4 M), 12 months (12 M) and 19+ months (19 M+) and on females at 12 M and 18 M+. Two months after surgery, operated and unoperated contralateral knees were harvested and evaluated using cartilage histology scores and muCT quantification of subchondral bone plate thickness and osteophyte formation. The 12 M and 19 M+ male mice developed more cartilage erosions and thicker subchondral bone plates after DMM than 4 M males. The size of osteophytes trended up with age, while the bone volume fraction was significantly higher in the 19 M+ group. Furthermore, 12 M females developed milder OA than males as indicated by less cartilage degradation, less subchondral bone plate sclerosis and smaller osteophytes. Our results reveal distinct age/gender-dependent structural changes in joint cartilage and subchondral bone post-DMM, facilitating more thoughtful selection of murine age/gender when using this surgical technique for translational OA research

NEW TOOLS FOR ENHANCED DIAGNOSTICS OF DGA DATA

In the last decade there has been a significant change in the way transformers are viewed. Their importance together with their obvious value to the network has been enhanced and recognized, especially in light of the ageing fleet worldwide. At the other end of the spectrum, new transformers are now being designed and built to tighter tolerances as a result of competitive market conditions, with the knock-on effect that these “modern” devices do not appear to provide the same stability and longevity as those that were entering service in the 1970s and 1980s. Against this backdrop, the advent of transformer monitoring has emerged and continues to develop at a rapid pace. Although still considered an emerging component of asset management practice, online DGA is rapidly gaining acceptance and recognition as one of the most powerful tools in protection against asset failures. While other transformer monitoring technologies abound, many of them now online, such as partial discharge, these products collectively combine to enable the move to condition based monitoring of transformer assets. As online DGA monitors have evolved new products and technologies are reaching the market at an ever increasing rate. However, the quiet revolution is in the analysis of the data. As more and more monitors are installed, so the burden of data analysis becomes increasingly large. New ways of extracting value from this data required. One important approach is the use of Artificial Neural Networks (ANN) for DGA data analysis. Additionally, with the recognition that data from monitors must be easily transferred into meaningful information for the end-user, diagnostic tools, such as the Duval Triangle, have evolved where the addition of Triangles 4 and 5 brings significantly more value to previously mined data. The mute question in this paper relates to whether or not existing online monitoring hardware has sufficient accuracy and repeatability of measurement to be of use with these more advanced diagnostic tools

Conformational effects of nucleotide exchange in ras p21 proteins as studied by fluorescence spectroscopy

AbstractThe intrinsic fluorescence properties of the oncogene protein p21N-ras, p21H-ras and one of its transforming mutants, p21N-ras (Va1112), have been investigated. A mutant containing a single tryptophan at position 28 in p21H-ras (Trp28) has been specifically engineered to provide a probe of protein conformation on nucleotide binding. The proteins produced essentially similar circular dichroism spectra typical of alpha/beta proteins. A decrease in the intensity of the fluorescence emission spectrum due to tyrosine occurred on GDP/GTP nucleotide exchange in the native and mutant proteins. Selective excitation of the single tryptophan in p21 produced a decrease in fluorescence intensity which was accompanied by a blue shift in the wavelength of maximum emission on nucleotide exchange. A reduction in the residual Mg2+ ion concentration enhanced this effect