08.25.14 - Dryad submission

08.25.14 - Dryad submissio

qRT-PCR primers and associated genes.

<p>qRT-PCR primers and associated genes.</p

Volume plot distribution of transcripts.

<p>Volume plot distribution of transcripts.</p

Significantly differentially expressed transcripts classified by ontology.

<p>Transcripts with significant upregulation or downregulation during diapause.</p

Expression abundance of diapausing vs. non-diapausing females of <i>Cx</i>. <i>pipiens</i> at 7 days after adult eclosion via quantitative real-time PCR.

<p>Ribosomal protein large subunit 19 (RpL19) as a loading control. Error bars represent standard error, n = 3.</p

Total genes upregulated and downregulated in diapausing females of <i>Cx</i>. <i>pipiens</i>.

<p>Total genes upregulated and downregulated in diapausing females of <i>Cx</i>. <i>pipiens</i>.</p

Volcano plot of log₂ fold change vs statistical significance.

<p>Volcano plot of log₂ fold change vs statistical significance.</p

Hypothetical proteins and their positions on the <i>Culex quinquefasciatus</i> (Johannesburg strain) reference genome.

<p>Hypothetical proteins and their positions on the <i>Culex quinquefasciatus</i> (Johannesburg strain) reference genome.</p

A transcriptomic survey of the impact of environmental stress on response to dengue virus in the mosquito, <i>Aedes aegypti</i>

<div><p>Populations of <i>Aedes aegypti</i> naturally exhibit variable susceptibility to dengue viruses. This natural variation can be impacted by nutritional stress resulting from larval-stage crowding, indicating the influence of environment components on the adult mosquito immune response. In particular, larval crowding was previously shown to reduce the susceptibility of adult females of a Trinidad field isolate of <i>A</i>. <i>aegypti</i> to the dengue serotype 2 (JAM1409) virus. Here, we present the first whole transcriptome study to address the impact of environmental stress on <i>A</i>. <i>aegypti</i> response to dengue virus. We examined expression profiles of adult females resulting from crowded and optimum reared larvae from the same Trinidad isolate at two critical early time points—3 and 18 hours post dengue virus infected blood meal. We exposed specimens to either a dengue or naïve blood meal, and then characterized the response in ten gene co-expression modules based on their transcriptional associations with environmental stress and time. We further analyzed the top 30 hub or master regulatory genes in each of the modules, and validated our results via qRT-PCR. These hub genes reveal which functions are critical to the mechanisms that confer dengue virus refractoriness or susceptibility to stress conditioned <i>A</i>. <i>aegypti</i>, as well as the time points at which they are most important.</p></div

Up to top 4 GO annotation clusters for general response modules.

<p>P-values adjusted by false discovery rate.</p